CD105 / Endoglin antibody

Principal name

CD105 / Endoglin antibody

Alternative names for CD105 / Endoglin antibody

ENG, END, HHT1, ORW, ORW1

SwissProt ID

P17813 (Human), P37176 (Pig), Q63961 (Mouse)

Gene ID

2022 (ENG), 13805 (Eng), 1202231 (EFA0061)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Eq (Equine), Mky (Monkey), Por (Porcine), Can (Canine)

Available hosts

Mouse, Rabbit, Rat, Chicken

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Functional assay (FN), Luminex (LMNX)

Background of CD105 / Endoglin antibody

A DNA sequence encoding the extracellular domain of mouse Endoglin (Met 1 - Gly 581) was expressed in insect cells. Mouse Endoglin is a disulfide-linked homodimeric protein. Based on N-terminal sequence analysis, the primary structure of recombinant mature Endoglin starts at Glu 26. Endoglin has a calculated monomeric molecular mass of 61 kDa but as a result of glycosylation, migrates at approximately 75 - 85 kDa under reducing conditions in SDS-PAGE. Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human endoglin, the S-endoglin and L-endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70% and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. In common with betaglycan (also named TÿRIII), a proteoglycan that shares regions of sequence similarity, endoglin is an accessory receptor for the TGF-β superfamily ligands. Endoglin does not bind ligands by itself, but does so by associating with a ligand-binding serine/threonine kinase receptor. Endoglin binds TGF-β 1 and TGF-β 3 but not TGF-β 2 efficiently by associating with TGF-β type II receptor (TβRII). It interacts with activin-A and BMP-7 using either the activin type II or type IIB receptors. In the case of BMP-2 which binds directly to the type I but not the type II BMP receptor, endoglin binds via either BMPR-IA (ALK-3) or BMPR-1B (ALK-6). Although the consequence of endoglin interactions on the functions of TGF-β family ligands is poorly understood, endoglin has clearly been shown to be required for angiogenesis and to play a key role in heart development. Mutations in human endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.

General readings

1. Haruta, Y. & Seon, B.K. (1986) Distinct human leukemia-associated cell surface glycoprotein
GP160 defined by monoclonal antibody SN6. Proc Natl Acad Sci U S A. 83 (20): 7898-902.
2. Jin, H.J. et al. (2010) GD2 expression is closely associated with neuronal differentiation of
human umbilical cord blood-derived mesenchymal stem cells. Cell Mol Life Sci. 67 (11):1845-58.
3. Nagano, M. et al. (2007) Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 110 (1): 151-60.
4. Braun, J. et al. (2010) Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells. Am J Vet Res. 71 (10):1228-36.
5. Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells. J Cell Physiol. 214: 75-83.
6. Agha-Hosseini, F. et al. (2010) In vitro isolation of stem cells derived from human dental pulp. Clin Transplant. 24: E23-8.
7. Arufe, M.C. et al. (2010) Chondrogenic potential of subpopulations of cells expressing mesenchymal stem cell markers derived from human synovial membranes. J Cell Biochem.111: 834-45.
8. Balmayor, E.R. et al. (2011) Synthesis and functionalization of superparamagnetic polyεcaprolactone
microparticles for the selective isolation of subpopulations of human adiposederived stem cells. J R Soc Interface. 8: 896-908.
9. Benetti, A. et al. (2008) Transforming growth factor-beta1 and CD105 promote the migration
of hepatocellular carcinoma-derived endothelium. Cancer Res. 68: 8626-34.
10. Ciccocioppo, R. et al. (2011) Autologous bone marrow-derived mesenchymal stromal cells in the treatment of fistulising Crohn's disease. Gut. 60: 788-98.
11. Cox, G. et al. (2011) The use of the reamer-irrigator-aspirator to harvest mesenchymal stem cells. J
Bone Joint Surg Br. 93: 517-24.
12. Ferro, F. et al. (2010) Biochemical and biophysical analyses of tissue-engineered bone obtained from three-dimensional culture of a subset of bone marrow mesenchymal stem cells. Page 2 of 4 Tissue Eng Part A. 16: 3657-67.
13. Lozanoska-Ochser, B. et al. (2008) Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration. J Immunol. 181: 6109-16.
14. Sallustio, F. et al. (2010) TLR2 plays a role in the activation of human resident renal stem/progenitor cells. FASEB J. 24: 514-25.
15. Tso, C. et al. (2012) Phenotypic and functional changes in blood monocytes following adherence to endothelium. PLoS One. 7: e37091.
16. Hu, N. et al. (2013) Long-term outcome of the repair of 50 mm long median nerve defects in rhesus monkeys with marrow mesenchymal stem cells-containing, chitosan-based tissue engineered nerve grafts Biomaterials. 34: 100-11.
17. Cho, H.J. et al. (2013) Generation of human secondary cardiospheres as a potent cell processing strategy for cell-based cardiac repair. Biomaterials. 34: 651-61.
18. Kang, S.D. et al. (2013) Isolation of Functional Human Endothelial Cells from Small Volumes of Umbilical Cord Blood. Ann Biomed Eng. 41: 2181-92.
19. Mehrkens, A. et al. (2013) Non-adherent mesenchymal progenitors from adipose tissue stromal vascular fraction. Tissue Eng Part A. 20: 1081-8.
20. De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry. Cytometry A. 81: 312-23.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD105 / Endoglin antibody

Immunofluorescent staining of HeLa cells using anti-ENG mouse monoclonal antibody (TA506170). Mouse IgG2b OTI8A1 Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD105 / Endoglin antibody

Immunofluorescence staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue). Mouse IgG2a MEM-229 Purified Hu, Por C, F, ICC/IF, WB
0.1 mg / €220.00
  Acris Antibodies GmbH

CD105 / Endoglin antibody

ENG ELISA with 6G8 Capture (TA600221) and 8A1 Detection (TA700220) Antibodies. Substrate used: Recombinant Human ENG (TP321669) Mouse IgG1 OTI6G8 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD105 / Endoglin antibody

ENG ELISA with 6G8 Capture (TA600221) and 3D5 Detection (TA700218) Antibodies. Substrate used: Recombinant Human ENG (TP321669) Mouse IgG1 OTI3D5 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

CD105 / Endoglin antibody

Immunohistochemical analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining. Mouse IgG1 3A9 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

CD105 / Endoglin antibody

Immunofluorescence staining of CD105 in mouse primary endothelial cells (SnoMec) with monoclonal rat anti-mouse CD105 Cat.-No DM3612P (Clone MJ7/18) (upper panel).Control with goat anti-rat ALEXA Flour 488 (lower panel). Rat IgG2a MJ7/18 Purified Ms C, F, IP, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD105 / Endoglin antibody

Double staining of human colon and colon carcinoma tissue with Endoglin (Cat.-No DP3517) / CD31. The experiments were perfomed by Dr. Ulrike Fiedler and Stefanie Koidel, Dept. of Vascular Biology and Angiogenesis Research, Tumor Biology Center, Breisacher Str. 117, D-79106 Freiburg, Germany Rabbit Purified Hu C, E, F, WB
0.2 mg / €290.00
  Acris Antibodies GmbH

CD105 / Endoglin antibody

Immunofluorescence staining (green) of CD105 in primary human dermal lymphatic endothelial cells (HDLEC) with anti-Human CD105 (0.5μg/ml) (Cat.-No DP3517P) and counter staining of nuclei with Dapi. As secondary antibody goat anti-rabbit ALEXA Flour 488 was used 1:400. Rabbit Aff - Purified Hu E, F, ICC/IF, WB
50 µg / €270.00
  Acris Antibodies GmbH

CD105 / Endoglin antibody

Double staining of human colon and colon carcinoma tissue with Endoglin (Cat.-No DP3517) / CD31. The experiments were perfomed by Dr. Ulrike Fiedler and Stefanie Koidel, Dept. of Vascular Biology and Angiogenesis Research, Tumor Biology Center, Breisacher Str. 117, D-79106 Freiburg, Germany Rabbit Purified Hu C, E, F, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

CD105 / Endoglin (26-176) antibody

AP15414PU CD105 antibody staining of Human Tonsil. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD105 / Endoglin (26-176) antibody

AP15414PU CD105 antibody staining of Human Tonsil. Rabbit Purified Hu P
1 ml / €440.00
  Acris Antibodies GmbH

CD105 / Endoglin (26-176) antibody

AP15414PU CD105 antibody staining of Human Tonsil. Rabbit Purified Hu P
0.1 ml / €180.00
  Acris Antibodies GmbH

CD105 / Endoglin antibody

Staining of bEnd.3 cell line with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD105 (Endoglin) Purified (filled histogram) followed by Anti-Rat IgG FITC .  Total viable cells were used for analysis. Rat MJ7/18 Purified Ms F, IP, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD105 / Endoglin antibody

Staining of U937 cell line with 0.5ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or Anti-Human CD105 (Endoglin) Purified (filled histogram) followed by F(ab')2 Anti-Mouse IgG PE .  Total viable cells were used for analysis. Mouse SN6 Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD105 / Endoglin antibody

Western blot analysis of extracts of various cell lines, using ENG antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD105 / Endoglin antibody

Western Blot: CD105 Antibody (3A9) [TA336920] - Western blot analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 3A9 Purified Hu E, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD105 / Endoglin antibody

Immunofluorescent staining of HeLa cells using anti-ENG mouse monoclonal antibody (TA506144). Mouse IgG1 OTI6G8 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD105 / Endoglin antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ENG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506145) Mouse IgG1 OTI3H5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD105 / Endoglin antibody

Immunofluorescent staining of HeLa cells using anti-ENG mouse monoclonal antibody (TA506167). Mouse IgG2b OTI3D5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD105 / Endoglin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENG (RC221699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENG. Mouse IgG1 OTI8B8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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