CD106 / VCAM1 antibody

Principal name

CD106 / VCAM1 antibody

Alternative names for CD106 / VCAM1 antibody

V-CAM 1, INCAM-100, L1CAM, VCAM-1, Vascular cell adhesion protein 1

SwissProt ID

P19320 (Human), P29533 (Mouse), P29534 (Rat), Q28260 (Canfa)

Gene ID

22329 (Vcam1), 7412 (VCAM1)

Available reactivities

Ms (Mouse), Hu (Human), Mky (Monkey), Por (Porcine), Rt (Rat), Can (Canine)

Available hosts

Rat, Mouse, Rabbit, Sheep, Goat

Available applications

Flow Cytometry (F), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Functional assay (FN), ELISA (capture) (E(capture)), Immunocytochemistry/Immunofluorescence (ICC/IF), Luminex (LMNX)

Background of CD106 / VCAM1 antibody

Human Vascular Cell Adhesion Molecule-1 (VCAM-1) is a 100 - 110 kDa, 715 amino acid (aa) type I transmembrane glycoprotein typically characterized by the presence of seven C2-type immunoglobulin (Ig) domains . Its extracellular region is 674 aa in length, followed by a22 aa transmembrane segment and a 19 aa cytoplasmic tail . In the extracellular region, there are multiple N-linked glycosylation sites (the predicted molecular weight is 80 kDa), and each C2 domain is closed by a disulfide bridge. There is considerable interspecies VCAM-1 homology, with mouse and rat VCAM-1 showing approximately 75% aa identity to humanVCAM-1 . Notably, the short 19 aa cytoplasmic tail is absolutely conserved, mouse to human to rat . Cells expressing mouse VCAM-1 bind both mouse and human leukocytes, and this reflects their high degree of aa identity . A number of variants of VCAM-1 are known to occur, all of which are likely the result of alternate gene splicing. In particular, a human six Ig domain molecule is known , and in rabbits, an eight Ig domain form has been identified . There is also a three-C2 domain, 43 kDa GPI-linked form of VCAM-1 . Although it binds known VCAM-1 ligands (or co-receptors), its function is unclear. Cells known to express VCAM-1 include neurons , endothelial cells , smooth muscle cells , fibroblasts and macrophages . Soluble VCAM-1 has been identified in culture supernates , blood , and cerebrospinal fluid . In vitro, basal levels of VCAM-1 shedding by unstimulated NIH3T3 cells appear to partially require metalloproteinase activity, while PMA-induced shedding is dependent upon the proteolytic activity of TACE/ADAM17 .

Functionally, VCAM-1 binds to both a4b1 (VLA-4) and a4b7 (LPAM-1) integrins . These integrins (or VCAM-1 ligands) are expressed on a variety of cells, with VLA-4 found on all leukocytes with the exception of neutrophils . Because of this, VCAM-1/VCAM-1 ligand interactions are undoubtedly key events in the rate and timing of leukocyte extravasation. Other roles proposed for VCAM-1 include the regulation of osteoclastogenesis via a cell-to-cell contact mechanism and the induction of sickle cell adherence to vascular endothelial cells during hypoxemia .

General readings

Hogg, N. et al. (1991) Chem. Immunol. 50:98.

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Reilly, P.L. et al. (1995) J. Immunol. 155:529.

Robledo, O. et al. (2003) Eur. J. Immunol. 33:1351.

Sithu, S.D. et al. (2007) J. Biol. Chem. 282:25010.

Fiore, E. et al. (2002) Oncogene 21:5213.

Tsakadze, N.L. et al. (2006) J. Biol. Chem. 281:3157.

Jun, C.D. et al. (2001) Proc. Natl. Acad. Sci. 98:6830.

Miller, J. et al. (1995) J. Exp. Med. 182:1231.

Diamond, M.S. et al. (1990) J. Cell Biol. 111:3129.

Rosenstein, Y. et al. (1991) Nature 354:233.

Pluskota, E. and S.E. D’Souza (2000) Eur. J. Biochem. 267:4693.

McCourt, P.A.G. et al. (1994) J. Biol. Chem. 269:30081.

Kirchberger, S. et al. (2006) Immunobiology 211:537.

Chakravorty S.J. and A. Craig (2005) Eur J. Cell Biol. 84:15.

Dietrich, J.B. (2002) J. Neuroimmunol. 128:58.

Humlicek, A.L. et al. (2004) Am. J. Pysiol. Lung Cell Mol. Physiol. 287:L598.

Steeber, D.A. et al. (1999) J. Immunol. 163:2176.

Forbes, E. et al. (2006) J. Leukoc. Biol. 80:330.

Lebedeva, T. et al. (2005) Curr. Opin. Immunol. 17:251.

Chirathaworn, C. et al. (2002) J. Immunol. 168:5530.

Camacho, S.A. et al. (2001) Nat. Immunol. 2:523.

Langston, W. et al. (2007) Free Rad. Biol. Med. 42:720.

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Rothlein, R. et al. (1991) J. Immunol. 147:3788.

Jander, S. et al. (1993) Neurology 43:1809.

Shijubo, N. et al. (1994) Clin. Exp. Immunol. 95:156.

Teppo, A.M. et al. (2001) Transplantation 71:1113.

Melis, M. et al. (2003) Am. J. Respir. Crit. Care Med. 167:1131.

Benson, V. et al. (2007) Curr. Mol. Med. 7:219.

Hoogeveen, R.C. et al. (2007) Diabetologia 50:36.

Covarrubias, M. et al. (2007) Am. J. Transplant. 7:2

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD106 / VCAM1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502391) Mouse IgG1 OTI3H10 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

Western Blot: CD106 / VCAM1 Antibody (6G9) [TA336775] - using VCAM1 mouse mAb against HUVEC (1) and EC (2) cell lysate. Mouse IgG1 6G9 Purified Hu C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502360) Mouse IgG1 OTI4E8 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 Antibody Cat.-No AM33117PU (Clone 1.4C3) at 1/100 using peroxidase-conjugate and DAB chromogen. Note membrane staining. Mouse IgG1 1.4C3 Purified Hu P
0.2 mg / €390.00
  Acris Antibodies GmbH

CD106 / VCAM1 antibody

Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 Antibody Cat.-No AM33117PU (Clone 1.4C3) at 1/100 using peroxidase-conjugate and DAB chromogen. Note membrane staining. Mouse IgG1 1.4C3 Purified Hu P
0.1 mg / €320.00
  Acris Antibodies GmbH

CD106 / VCAM1 antibody

Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 Antibody Cat.-No AM33117PU (Clone 1.4C3) at 1/100 using peroxidase-conjugate and DAB chromogen. Note membrane staining. Mouse IgG1 1.4C3 Purified Hu P
20 µg / €160.00
  Acris Antibodies GmbH

CD106 / VCAM1 antibody

AP32710PU-N VCAM1 Antibody staining of Human Uterus lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD106 / VCAM1 antibody

Staining of rat bone marrow cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Rat CD106 (VCAM-1) Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE .  Cells in the large scatter population were used for analysis. Mouse eBioMR106 (MR106) Purified Rt F, IP, P, WB
50 µg / €120.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

CD106 / VCAM1 Mouse STA Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

Western blot analysis of extracts of various cell lines , using VCAM1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

To detect hVCAM-1 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hVCAM-1. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

To detect hVCAM-1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human VCAM-1 (TA328468) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVCAM-1. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

CD106 / VCAM1 Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

TNF-alpha; stimulated HUVEC cells stained with purified STA, followed by anti-mouse IgGs FITC Mouse STA Purified Hu E, F, IP, P
0.1 mg / €248.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

C57BL/6 mouse bone marrow myeloid cells stained with 429 purified, followed by anti-rat IgG FITC. Rat 429 (MVCAM.A) Purified Ms F, IP, P
50 µg / €248.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (RC209761, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1. Mouse IgG1 OTI4F1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (RC209761, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1. Mouse IgG1 OTI4A10 Purified Can, Hu, Mky F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502392) Mouse IgG1 OTI5B12 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

Anti-VCAM1 mouse monoclonal antibody (TA502411) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VCAM1(RC209761). Mouse IgG1 OTI2G5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502426) Mouse IgG1 OTI3G7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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