CD106 / VCAM1 antibody

Principal name

CD106 / VCAM1 antibody

Alternative names for CD106 / VCAM1 antibody

V-CAM 1, INCAM-100, L1CAM, VCAM-1, Vascular cell adhesion protein 1

SwissProt ID

P19320 (Human), P29533 (Mouse), P29534 (Rat), Q28260 (Canfa)

Gene ID

7412 (VCAM1), 22329 (Vcam1), 25361

Available reactivities

Hu (Human), Ms (Mouse), Mky (Monkey), Por (Porcine), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rat, Rabbit, Sheep, Goat

Available applications

Frozen Sections (C), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Neutralisation (NEUT), ELISA (capture) (E(capture)), Luminex (LMNX)

Background of CD106 / VCAM1 antibody

The monoclonal antibody 6C7.1 recognizes mouse vascular cell adhesion molecule (VCAM-1) (~ 81 kDa), a member of a subclass of the immunoglobulin superfamily (IgSF). IgSF members are ligands for integrins. Cell adhesion molecules (CAMs) have important roles in the immune response, immune surveillance and cell-cell recognition, especially in leukocyte-endothelial cell adhesion. CAMs on the surface of leukocytes and endothelial cells are actively involved in the recruitment of specific leukocyte subsets into different tissues. VCAM-1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Cell adhesion molecules, like VCAM-1, are upregulated on cerebral vessels during inflammatory conditions of the central nervous system such as experimental autoimmune encephalomyelitis (EAE), a model system for multiple sclerosis. Administration of monoclonal antibody 6C7.1 has been shown to inhibit or diminish clinical or pathological signs of EAE. VCAM-1 is a receptor for encephalomyocarditis virus on murine vascular endothelial cells. Expression of VCAM-1 on vascular endothelial cells is induced by TNF-alpha, IL-1, IFN-gamma or endotoxin. VCAM-1 is a ligand for the integrins alpha4beta1 (VLA-4) and alpha4beta7 (LPAM-1). These integrins are constitutively expressed by thymocytes, lymphocytes and monocytes. VCAM-1/VLA-4 interaction may play a pathophysiological role in immune responses and as well as in leukocyte emigration to sites of inflammation.

General readings

Huber SA., et al. (1994) J Virol. 68:3453-3458.Preiss DJ., et al. (2007) Int J Clin Pract. 61(4): 697-701.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD106 / VCAM1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502391) Mouse IgG1 OTI3H10 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

Western Blot: CD106 / VCAM1 Antibody (6G9) [TA336775] - using VCAM1 mouse mAb against HUVEC (1) and EC (2) cell lysate. Mouse IgG1 6G9 Purified Hu C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502360) Mouse IgG1 OTI4E8 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 Antibody Cat.-No AM33117PU (Clone 1.4C3) at 1/100 using peroxidase-conjugate and DAB chromogen. Note membrane staining. Mouse IgG1 1.4C3 Purified Hu P
0.2 mg / €390.00
  Acris Antibodies GmbH

CD106 / VCAM1 antibody

Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 Antibody Cat.-No AM33117PU (Clone 1.4C3) at 1/100 using peroxidase-conjugate and DAB chromogen. Note membrane staining. Mouse IgG1 1.4C3 Purified Hu P
0.1 mg / €320.00
  Acris Antibodies GmbH

CD106 / VCAM1 antibody

Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 Antibody Cat.-No AM33117PU (Clone 1.4C3) at 1/100 using peroxidase-conjugate and DAB chromogen. Note membrane staining. Mouse IgG1 1.4C3 Purified Hu P
20 µg / €160.00
  Acris Antibodies GmbH

CD106 / VCAM1 antibody

AP32710PU-N VCAM1 Antibody staining of Human Uterus lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CD106 / VCAM1 antibody

Staining of rat bone marrow cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Rat CD106 (VCAM-1) Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE .  Cells in the large scatter population were used for analysis. Mouse eBioMR106 (MR106) Purified Rt F, IP, P, WB
50 µg / €120.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

CD106 / VCAM1 Mouse STA Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

Western blot analysis of extracts of various cell lines , using VCAM1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

To detect hVCAM-1 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hVCAM-1. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

To detect hVCAM-1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human VCAM-1 (TA328468) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVCAM-1. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (RC209761, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1. Mouse IgG1 OTI4F1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (RC209761, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1. Mouse IgG1 OTI4A10 Purified Can, Hu, Mky F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502392) Mouse IgG1 OTI5B12 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

Anti-VCAM1 mouse monoclonal antibody (TA502411) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VCAM1(RC209761). Mouse IgG1 OTI2G5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502426) Mouse IgG1 OTI3G7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD106 / VCAM1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (RC213353, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1. Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (RC213353, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD106 / VCAM1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (RC213353, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

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