CD107a / LAMP1 antibody

Principal name

CD107a / LAMP1 antibody

Alternative names for CD107a / LAMP1 antibody

LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1, CD107 Antigen-like Family Member A

SwissProt ID

P05300 (Chick), P11279 (Human), P11438 (Mouse), P14562 (Rat), Q05204 (Bovin), Q5K4G2 (Pig)

Gene ID

3916 (LAMP1), 16783 (Lamp1), 25328, 494565

Available reactivities

Hst (Hamster), Hu (Human), Rt (Rat), Ms (Mouse), Por (Porcine), Bov (Bovine), Mky (Monkey)

Available hosts

Mouse, Rabbit, Rat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunoelectron Microscopy (EM), Enzyme Immunoassay (E)

Background of CD107a / LAMP1 antibody

LAMP1 is a protein primarily associated with the lysosomal membrane. Antibodies to LAMP1 are therefore excellent markers of lysosomes in mammalian cells, though some LAMP1 may also be seen on late endosomes and on the plasma membrane. In a typical cell LAMP1 is associated with spherical vesicles located next to the nucleus and the microtubule organizing center. The protein is also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA, as the protein was independently discovered and med by several different labs. CD is an abbreviation for "Cluster of Differentiation" and refers to a protocol for the ming of proteins and other surface markers of membranes of human leucocytes defined by binding of specific monoclol antibodies. LAMP1 is found on the cell surface of lymphocytes undergoing degranulation, a process in which cytoplasmic vesicles fuse with the plasma membrane, and this phenome resulted in discovery of LAMP1 as a CD protein. The LAMP1 protein has a large N-termil region which is inside the lysosome, hence topologically exterl to the cell, which is often referred to as the lumel domain. The lumel domain consists of two homologous globular segments separated by a proline rich sequence. Next there is a single membrane spanning domain and a short 11 amino acid C-termil cytoplasmic tail. This tail region contains, at the extreme C-terminus, a so-called YXXI motif which is responsible for the sorting of the intact molecule to the endosome and lysozome, where Y = tyrosine, I = isoleucine and X = almost any amino acid. This motif is found in several other lysosomal proteins, where it functions in the same way. There are 417 amino acids in the human LAMP1 molecule, giving a tive molecular weight of 44.8kDa. However the N-termil lumel segment of LAMP1 is very heavily and variably glycosylated due to the presence of 18 N-linked glycosylation sites, so that on SDS-PAGE and on Western blots the protein runs as a diffuse band at 90-120kDa.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD107a / LAMP1 antibody

Anti-LAMP1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

CD107a / LAMP1 antibody

Western blot analysis of extracts of various cell lines, using LAMP1 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD107a / LAMP1 antibody

Immunohistochemistry-Paraffin: LAMP1 Antibody [NBP1-46395] - Rat spinal cord (paraffin section) using Rabbit antibody to second lumenal domain of Lamp1 (CD107a): whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. Rabbit Purified Ms, Rt P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

CD107a / LAMP1 antibody

Western blot analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD107a / LAMP1 antibody

Western blot analysis of extracts from K562, Jurkat, HepG2, HuvEc, HeLa and COLO205 cells, using LAMP1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD107a / LAMP1 (N-term) antibody

Western blot analysis of lysates from Hela,HT1080 cell line and human placenta tissue (from left to right),using LAMP1 Antibody (N-term)(Cat. #TA324642).TA324642 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CD107a / LAMP1 antibody

Western blot analysis of LAMP1 in rat liver microsomes, using a 1:1000 dilution of the antibody Mouse IgG1 Ly1C6 Purified Hst, Rt ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD107a / LAMP1 antibody

Western Blot: LAMP1 Antibody (5H6) [TA337107] - Western strip blots of HeLa cell crude extracts stained with LAMP1 antibody TA337107 in lane 9. Lane 10 shows staining with our other LAMP1 antibody NBP2-25155. Both antibodies bind to a diffuse band running Mouse IgG1 5H6 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD107a / LAMP1 antibody

Western Blot: LAMP1 Antibody (6E2) [TA337108] - Western strip blots of HeLa cell crude extracts with two different preparations of TA337108 in lanes 17 and 18. Both preparations bind to a diffuse band running at between ~90kDa and ~120kDa as expected, and Mouse IgG1 6E2 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD107a / LAMP1 antibody

CD107a / LAMP1 Mouse IgG1 B-T47 Azide Free Hu C, F
0.1 mg / €250.00
  Acris Antibodies GmbH

CD107a / LAMP1 antibody

CD107a / LAMP1 Mouse IgG1 B-T47 FITC Hu F
1ml (100T) / €270.00
  Acris Antibodies GmbH

CD107a / LAMP1 antibody

Western Blot analysis using CD107a / LAMP1 Antibody Cat.-No AM03025PU-N (Clone B-T47) at 2 µg/ml in 1 mg/ml lysate, HeLa 10 µg (Lane 1+2), Raw 20µg (Lane 3+4) lysate, blocking buffer (Blotto; 5% dry milk powder in TBST). R1253HRP anti-Mouse IgG (H+L)-HRP as secondary antibody. Mouse IgG1 B-T47 Purified Hu F, ICC/IF, WB
2ml (200T) / €250.00
  Acris Antibodies GmbH

CD107a / LAMP1 antibody

Western blot analysis of LAMP1 in rat liver microsomes, using a 1:1000 dilution of the antibody Mouse IgG1 Ly1C6 Purified Hst, Rt ICC/IF, IP, WB
25 µg / €250.00
  Acris Antibodies GmbH

CD107a / LAMP1 antibody

CD107a / LAMP1 Mouse IgG1 4E9/11 FITC Por F
0.1 mg / €530.00
  Acris Antibodies GmbH

CD107a / LAMP1 antibody

CD107a / LAMP1 Rat IgG2a 1D4B APC Ms F
0.1 mg / €290.00
  Acris Antibodies GmbH

CD107a / LAMP1 antibody

CD107a / LAMP1 Rat IgG2a 1D4B FITC Ms F
0.5 mg / €380.00
  Acris Antibodies GmbH

CD107a / LAMP1 antibody

CD107a / LAMP1 Rat IgG2a 1D4B Purified Ms C, EM, F, IP, WB
0.5 mg / €330.00
  Acris Antibodies GmbH

CD107a / LAMP1 antibody

CD107a / LAMP1 Mouse IgG1 H4A3 Biotin Hu F
100 Tests / €280.00
  Acris Antibodies GmbH

CD107a / LAMP1 antibody

CD107a / LAMP1 Mouse IgG1 H4A3 FITC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD107a / LAMP1 antibody

Western Blot analysis using CD107a / LAMP1 Antibody Cat.-No AM08169PU-N (Clone H4A3) at 2 µg/ml in 1 mg/ml lysate, HeLa 10 µg (Lane 1+2), Raw 20 µg (Lane 3+4) lysate, blocking buffer (Blotto; 5% dry milk powder in TBST). R1253HRP anti-Mouse IgG (H+L)-HRP as secondary antibody. Mouse IgG1 H4A3 Purified Hu C, F, WB
0.1 mg / €190.00
  Acris Antibodies GmbH

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