CD113 / Nectin 3 antibody

Principal name

CD113 / Nectin 3 antibody

Alternative names for CD113 / Nectin 3 antibody

Nectin-3, CDw113, PVRL3, PRR3, Poliovirus receptor-related protein 3

SwissProt ID

Q9JLB9 (Mouse), Q9NQS3 (Human)

Gene ID

25945 (PVRL3), 58998 (Pvrl3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Functional assay (FN), Paraffin Sections (P)

Background of CD113 / Nectin 3 antibody

Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calcium-independent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3α, -3β and -3g of which nectin-3α is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253 (2010).
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642 (2009).
Yu, X., et al. Nat. Immunol. 10(1):48-57 (2009).
Fujito, T., et al. J. Cell Biol. 171(1):165-173 (2005).

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD113 / Nectin 3 antibody

  Rabbit Polyclonal Anti-PVRL3 Antibody  
WB Suggested Anti-PVRL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD113 / Nectin 3 antibody

  Rabbit Polyclonal Anti-PVRL3 Antibody  
WB Suggested Anti-PVRL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD113 / Nectin 3 antibody

  Rabbit Polyclonal Anti-PVRL3 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human placenta tissue, NIH/3T3 cells, Primary antibody: (PVRL3 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD113 / Nectin 3 (extracell. dom.) (50-400) antibody

CD113 / Nectin 3 Rat IgG2a 103-A1 FITC Ms C, E, FN, ICC/IF, IP
0.1 mg / €500.00
  Acris Antibodies GmbH

CD113 / Nectin 3 (extracell. dom.) (50-400) antibody

CD113 / Nectin 3 Rat IgG2a 103-A1 Purified Ms C, E, FN, ICC/IF, IP
0.1 mg / €460.00
  Acris Antibodies GmbH

CD113 / Nectin 3 (C-term) antibody

Western blot analysis of CD113 / Nectin 3 Antibody (C-term) Cat.-No AP53524PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the PVRL3 antibody detected the PVRL3 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD113 / Nectin 3 antibody

Western blot analysis of extracts from A549 cells, using PVRL3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €230.00
  Assay Biotech

CD113 / Nectin 3 antibody

Western blot analysis of extracts from A549 cells, using PVRL3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

PVRL3 - human gene knockout kit via CRISPR

PVRL3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PVRL3 - human gene knockout kit via CRISPR

PVRL3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PVRL3 - human gene knockout kit via CRISPR

PVRL3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PVRL3 - human gene knockout kit via CRISPR

PVRL3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pvrl3 - mouse gene knockout kit via CRISPR

Pvrl3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pvrl3 - mouse gene knockout kit via CRISPR

Pvrl3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pvrl3 - mouse gene knockout kit via CRISPR

Pvrl3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pvrl3 - mouse gene knockout kit via CRISPR

Pvrl3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD113 / Nectin 3

  Recombinant protein of human poliovirus receptor-related 3 (PVRL3)  
CD113 / Nectin 3 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €399.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PVRL3 overexpression lysate

PVRL3 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PVRL3 (mGFP-tagged) - Human poliovirus receptor-related 3 (PVRL3), 200 uL, >10^7 TU/mL

Lenti ORF particles, PVRL3 (mGFP-tagged) - Human poliovirus receptor-related 3 (PVRL3), 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

Lenti ORF particles, PVRL3 (Myc-DDK tagged) - Human poliovirus receptor-related 3 (PVRL3) , 200 uL, >10^7 TU/mL

Lenti ORF particles, PVRL3 (Myc-DDK tagged) - Human poliovirus receptor-related 3 (PVRL3) , 200 uL, >10^7 TU/mL  
200 µl / €1,060.00
  OriGene Technologies, Inc.

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn