CD114 / CSF3R antibody

Principal name

CD114 / CSF3R antibody

Alternative names for CD114 / CSF3R antibody

G-CSF-R, GCSFR, GCSF Receptor, G-CSF Receptor, Granulocyte colony-stimulating factor receptor

SwissProt ID

P40223 (Mouse), Q99062 (Human)

Gene ID

1441 (CSF3R), 100171441 (ACE2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Flow Cytometry (F), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of CD114 / CSF3R antibody

G-CSF R, also known as granulocyte colony-stimulating factor receptor and CD114 antigen, is a member of the cytokine receptor superfamily. Members of this family, which also includes the receptors for IL-2 to IL-7, GM-CSF, EPO, TPO and GH, are characterised by the presence of four conserved cysteine residues in their extracellular domains. The G CSF R extracellular domain has a composite structure containing an Ig-like domain, a cytokine receptor homologous region and fibronectin type III domains. The human G-CSF R mediates the biological activity of G-CSF which is unique in its ability to not only stimulate the proliferation but also potently induces the terminal maturation of myeloid progenitor cells to neutrophilic granulocytes. G-CSF R is produced as an ECD-Fc fusion protein with the aim of enhancing its activity. ECD-Fc fusion proteins have an advantage over soluble receptors because many receptors are only functional in dimeric form. Fusion to the Fc domain of IgG1 induces dimerization due to the ability of the Fc domain to form disulfide bonds. The resulting dimeric receptor ECD-Fc mimics the activated form of the receptor and possess enhanced affinity for its cognate ligand relative to its monomeric form. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Avalos BR (1996) Blood 88(3): 761-777

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD114 / CSF3R antibody

Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSF3R antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD114 / CSF3R antibody

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human lung cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSF3R antibody at dilution 1/12, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD114 / CSF3R antibody

Immunohistochemistry: GCSF Receptor Antibody - Most normal tissues were negative. Trophoblastic cells in placenta expressed strong cytoplasmic immunoreactivity. Myocardium, neuronal cells of cerebral cortex and Leydig cells showed moderate positivity. Among malignancies, most cancers were negative except for few adenocarcinomas of endometrium and one case of urothelial cancer that displayed moderate membranous staining in a fraction of cells.  Image and statement courtesy of the Human Protein Atlas (HPA). Rabbit IgG Purified Hu E, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD114 / CSF3R (551-563) antibody

AP33491PU-N  (1µg/ml) staining of Peripheral Blood Lymphocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CD114 / CSF3R (Center) antibody

Formalin-fixed, paraffin-embedded human colon carcinoma reacted with CD114 / CSF3R Antibody (Center) Cat.-No AP51098PU-N which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD114 / CSF3R antibody

CD114 / CSF3R Mouse IgG2b S-1390 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

CD114 / CSF3R antibody

CD114 / CSF3R Mouse IgG1 S-1268 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

CD114 / CSF3R antibody

CD114 / CSF3R Mouse IgG1 S-1252 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

CD114 / CSF3R antibody

CD114 / CSF3R Mouse IgG2a S-1284 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

CD114 / CSF3R antibody

CD114 / CSF3R Mouse IgG2a S-1374 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

CD114 / CSF3R antibody

CD114 / CSF3R Mouse IgG2a S-425 Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

CD114 / CSF3R antibody

CD114 / CSF3R Mouse IgG1 LMM775 PE Hu F
100 Tests / €480.00
  Acris Antibodies GmbH

CD114 / CSF3R antibody

CD114 / CSF3R Mouse IgG1 LMM775 PE Hu F
25 Tests / €220.00
  Acris Antibodies GmbH

CD114 / CSF3R antibody

CD114 / CSF3R Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CD114 / CSF3R antibody

Western Blot analysis of CSF3R expression in transfected 293T cell line (H00001441-T01) by CSF3R MaxPab polyclonal antibody.

Lane 1: CSF3R transfected lysate(91.96 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

CD114 / CSF3R antibody

Western Blot analysis of CSF3R expression in transfected 293T cell line (H00001441-T01) by CSF3R MaxPab polyclonal antibody.

Lane 1: CSF3R transfected lysate(91.96 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD114 / CSF3R antibody

CD114 / CSF3R Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

CD114 / CSF3R antibody

Western Blot analysis of CSF3R expression in transfected 293T cell line (H00001441-T02) by CSF3R MaxPab polyclonal antibody.

Lane 1: CSF3R transfected lysate(92.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CD114 / CSF3R antibody

CD114 / CSF3R Mouse IgG1 BDI542 Hu E, IP, WB
0.5 mg / €820.00
  Abnova Taiwan Corp.

CD114 / CSF3R antibody

CD114 / CSF3R Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

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