CD115 antibody

Principal name

CD115 antibody

Alternative names for CD115 antibody

CSF-1-R, Fms proto-oncogene, c-fms, CSF1R, FMS, Macrophage colony-stimulating factor 1 receptor

SwissProt ID

P07333 (Human), P09581 (Mouse), Q00495 (Rat)

Gene ID

1436 (CSF1R), 12978 (Csf1r)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Sh (Sheep), Chk (Chicken), Ze (Zebrafish), Mky (Monkey)

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Neutralisation (NEUT)

Background of CD115 antibody

CSF1R(Colony-Stimulating Factor 1 Receptor) also known as MCSFR, FMS, c-FMS, CD115, ONCOGENE FMS or CD115 ANTIGEN, encodes a tyrosine kinase growth factor receptor for colony-stimulating factor-1, the macrophage-and monocyte-specific growth factor. The gene is located on long arm of chromosome 5 (5q32) on the Crick (minus) strand. The as-yet-unidentified CSF1 Rpromoter/enhancer sequences may be confined to the nucleotides separating the 2 genes or could potentially lie within the PDGFR gene itself. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. The encoded protein is atyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Kondo et al. (2000) showed that the endogenous myelomonocytic cytokine receptors for GM-CSF and macrophage colony-stimulating factor (CSF1R) are expressed at low to moderate levels on the more primitive hematopoietic stem cells, are absent on common lymphoid progenitors, and are upregulated after myeloid lineage induction by IL2.

General readings

Gynecol Oncol. 2001 Feb;80(2):194-200.
The activated macrophage colony-stimulating factor (CSF-1) receptor as a predictor of poor outcome in advanced epithelial ovarian carcinoma.
Toy EP, Chambers JT, Kacinski BM, Flick MB, Chambers SK.
Department of Obstetrics and Gynecology (Gynecologic Oncology), Yale University, New Haven, Connecticut 06520, USA. eugene.toy@yale.edu

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD115 antibody

Western blot analysis of extracts from HT-29 cells, using CSFR (Ab-809) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD115 (734-744) antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with AP22414PU-N (1 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP22414PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CD115 (C-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CSF1R Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD115 (700-710) antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with TA311324 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1:1000) in Lane C. Mock-transfected HEK293 probed with TA311324 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD115 antibody

Western blot analysis of extracts from MCF-7 cells, using M-CSF Receptor (Ab-723) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD115 antibody

Western blot analysis of extracts from HepG2 cells, using CSFR (Ab-699)antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD115 antibody

Staining of normal human peripheral blood cells with 0.5 ug of Rat IgG2a K Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD115 (c-fms) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE .  Cells in the monocyte gate were used for analysis. Rat 12-3A3-1B10 Purified Hu F
25 µg / €120.00
  OriGene Technologies, Inc.

CD115 antibody

Staining of thioglycolate-induced peritoneal exudate cells (PECs) with 0.25 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD115 (c-fms) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE .  Total viable cells were used for analysis. Rat AFS98 Purified Ms F
50 µg / €120.00
  OriGene Technologies, Inc.

CD115 antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSF1R antibody at dilution 1/45, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD115 antibody

Predicted band size: 108 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD115 antibody

Western blot analysis of lysate from U-937 cell line,  using CSF1R Antibody(Cat.  #TA324948).  TA324948 was diluted at 1:1000.  A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.  Lysate at 35ug. Rabbit IgG Purified Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CD115 antibody

Western blot analysis of M-CSF Receptor expression in LPS treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD115 antibody

Western blot analysis on 293 cell lysate using M-CSF Receptor Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD115 antibody

CD115 Rat IgG1 604B5 2E11 Azide Free Ms, Rt F, FN
1 mg / €1,150.00
  Acris Antibodies GmbH

CD115 antibody

CD115 Rat IgG1 604B5 2E11 Azide Free Ms, Rt F, FN

Regular Price: 0.2 mg / €340.00

Special Price: 0.2 mg / €238.00

  Acris Antibodies GmbH

CD115 antibody

CD115 Rat IgG1 604B5 2E11 Purified Ms, Rt F
0.25 mg / €500.00
  Acris Antibodies GmbH

CD115 antibody

CD115 Rat IgG1 604B5 2E11 Purified Ms, Rt F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD115 antibody

CD115 Rat IgG1 604B5 2E11 Purified Ms, Rt F
25 µg / €190.00
  Acris Antibodies GmbH

CD115 antibody

Flow Cytometry: Staining of the mouse J774 cell line with Rat anti Mouse CD115 antibody RPE conjugated (AM05871RP-N) Rat IgG1 604B5 2E11 PE Ms, Rt F
100 Tests / €370.00
  Acris Antibodies GmbH

CD115 antibody

CD115 Rat IgG1 604B5 2E11 PE Ms, Rt F
25 Tests / €230.00
  Acris Antibodies GmbH

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