CD117 / c-kit antibody

Principal name

CD117 / c-kit antibody

Alternative names for CD117 / c-kit antibody

SCFR, KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase

SwissProt ID

O97799 (Canfa), P05532 (Mouse), P10721 (Human), P43481 (Bovin), Q08156 (Chick), Q2HWD6 (Pig)

Gene ID

16590 (Kit), 3815 (KIT), 378783

Available reactivities

Ms (Mouse), Can (Canine), Hu (Human), Mky (Monkey), Bov (Bovine), Rt (Rat), Por (Porcine), Chk (Chicken), Prim (Primate)

Available hosts

Rat, Rabbit, Mouse, Chicken, Goat

Available applications

Flow Cytometry (F), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Functional assay (FN), Neutralisation (NEUT), Dot blot (Dot), Luminex (LMNX)

Background of CD117 / c-kit antibody

Stem cell factor receptor (SCF R), also known as c-kit, has a molecular weight of 145 kDa as a mature protein and is a member of the type III receptor tyrosine kinase (RTK) family that includes platelet-derived growth factor (PDGF) receptors and the macrophage colony-stimulating factor 1 (CSF-1) (c-fms) receptor. SCF R is essential for the development of normal hematopoietic cells and plays an important role in the survival, proliferation, and differentiation of mast cells, melanocytes, and germ cells. It is expressed by hematopoietic cells in the embryonic liver throughout development, and by more committed progenitors, such as myeloid, erythroid, megakaryocytic, natural killer, and dendritic progenitor cells. SCF R contains five extracellular immunoglobulin-like (Ig) domains, a single juxtamembrane domain (JMD), a cytoplasmic tyrosine kinase domain interrupted by a large kinase insert (KI) in the ATP-binging region, and a carboxy tail. Binding of SCF R to its ligand (stem cell factor; SCF) induces receptor dimerization, trans autophosphorylation of the kinase domain, recruitment of signaling proteins via phosphotyrosine binding or Src homology 2 (SH2) domains, and subsequent signal transduction. A variety of human diseases are associated with the inappropriate expression or activation of SCF R, which can be classified as loss-of-function mutations or gain-of-function mutations. Activating mutations in SCF R have been primarily identified in the JMD and in the second part of the kinase domain, and are associated with gastrointestinal stromal cell tumors and mastocytosis, respectively. There are also reports of activating mutations in some forms of germ cell tumors. The overexpression of SCF R has previously been documented in myeloid leukemia, neuroblastoma, breast tumor, colon tumors, gynecological tumors, testicular germ cell tumors and small cell lung carcinoma (SCLC). Loss-of-function mutations in SCF R can result in diseases such as autosomal-dominant piebaldism, leading to deafness, megacolon, and abnormalities in pigmentation of skin and hair. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

This product has been used in:Arber DA et al. Paraffin section detection of the c-kit gene product (CD117) in human tissues: value in the diagnosis of mast cell disorders. Hum Pathol 29:498-504 (1998). PubMed PMID: 9596274
General / background references:Miettinen M et al. Gastrointestinal stromal tumors: recent advances in understanding of their biology. Hum Pathol 30:1213-20 (1999). PubMed PMID: 10534170
Escribano L et al. Sequential immunophenotypic analysis of mast cells in a case of systemic mast cell disease evolving to a mast cell leukemia. Cytometry 30:98-102 (1997). PubMed PMID: 9149916
Oertel J et al. Immunotyping of blasts in human bone marrow. Ann Hematol 72:125-9 (1996). PubMed PMID: 8766253

  1. Quick-Filters:
  2. Highly Validated (6)

Items 1 to 20 of 190 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD117 / c-kit antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KIT rabbit polyclonal antibody. (TA590744) Rabbit IgG Purified Hu E, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD117 / c-kit antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801036) Mouse IgG2a OTI2E3 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD117 / c-kit antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801037) Mouse IgG1 OTI1E2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD117 / c-kit antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808721)(1:150) Mouse IgG2b OTI1B6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD117 / c-kit antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-KIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808739)(1:150) Mouse IgG2b OTI2B12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD117 / c-kit antibody

Immunohistochemical analysis of paraffin-embedded gastrointestinal stromal tumor using KIT mouse mAb with DAB staining. Mouse IgG1 1C5 Ascites Hu E, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

CD117 / c-kit (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human gastrointestinal tissue (4 μm) stained with anti-CD117 antibody (DB 062) shows strong immunostaining of neoplastic cell in large bowel stromal tumor. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG R21-V Aff - Purified Hu P
0.1 ml / €270.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD117 / c-kit (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human gastrointestinal tissue (4 µm) stained with anti-CD117  antibody (DB 062) shows strong immunostaining of neoplastic cell in large bowel stromal tumor.  Kindly performed and provided by Katarina Poliakova, MD and .ubomir Straka, MD, PhD. from Clinical Pathology Pre.ov, Ltd., Pre.ov, Slovak republic. Rabbit IgG R21-V Aff - Purified Hu P
0.2 ml / €360.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD117 / c-kit (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human gastrointestinal tissue (4 μm) stained with anti-CD117 antibody (DB 062) shows strong immunostaining of neoplastic cell in large bowel stromal tumor. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG R21-V Aff - Purified Hu P
0.5 ml / €450.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD117 / c-kit (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human gastrointestinal tissue (4 μm) stained with anti-CD117 antibody (DB 062) shows strong immunostaining of neoplastic cell in large bowel stromal tumor. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG R21-V Aff - Purified Hu P
1 ml / €690.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD117 / c-kit antibody

Human gastrointestinal stromal tumor stained with anti-CD117 antibody Rabbit SP26 Supernatant Hu P
0.5 ml / €410.00
  Acris Antibodies GmbH

CD117 / c-kit antibody

Human gastrointestinal stromal tumor stained with anti-CD117 antibody Rabbit SP26 Supernatant Hu P
1 ml / €590.00
  Acris Antibodies GmbH

CD117 / c-kit antibody

Human gastrointestinal stromal tumor stained with anti-CD117 antibody Rabbit SP26 Supernatant Hu P
0.1 ml / €290.00
  Acris Antibodies GmbH

CD117 / c-kit antibody

Formalin-Fixed, Paraffin-Embedded Human gastro intestinal stromal tumor (GIST) stained with CD117 / C-Kit Antibody Cat.-No AM32824PU (Clone C117/370). Mouse IgG1 C117/370 Purified Hu P
0.2 mg / €390.00
  Acris Antibodies GmbH

CD117 / c-kit antibody

Formalin-Fixed, Paraffin-Embedded Human gastro intestinal stromal tumor (GIST) stained with CD117 / C-Kit Antibody Cat.-No AM32824PU (Clone C117/370). Mouse IgG1 C117/370 Purified Hu P
0.1 mg / €320.00
  Acris Antibodies GmbH

CD117 / c-kit antibody

Formalin-Fixed, Paraffin-Embedded Human gastro intestinal stromal tumor (GIST) stained with CD117 / C-Kit Antibody Cat.-No AM32824PU (Clone C117/370). Mouse IgG1 C117/370 Purified Hu E, P
20 µg / €160.00
  Acris Antibodies GmbH

CD117 / c-kit antibody

Human gastrointestinal stromal tumor stained with CD117/c-kit antibody (Cat# AP15416PU) Rabbit Purified Can, Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD117 / c-kit antibody

Human gastrointestinal stromal tumor stained with CD117/c-kit antibody (Cat# AP15416PU) Rabbit Purified Can, Hu P
1 ml / €490.00
  Acris Antibodies GmbH

CD117 / c-kit antibody

Human gastrointestinal stromal tumor stained with CD117/c-kit antibody (Cat# AP15416PU) Rabbit Purified Can, Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD117 / c-kit antibody

Anti-KIT (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Items 1 to 20 of 190 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  • LinkedIn