CD11a / ITGAL antibody

Principal name

CD11a / ITGAL antibody

Alternative names for CD11a / ITGAL antibody

Integrin alpha-L, LFA1, LFA-1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain, CD11 antigen-like family member A

SwissProt ID

B5AZS0 (Horse), F1P8D8, G1T7A0, P20701 (Human), P24063 (Mouse), P61625 (Bovin), Q004B3 (Sheep), Q3T1L6 (Rat)

Gene ID

3683 (ITGAL), 281874, 73683 (Atg16l2), 308995, 16408

Available reactivities

Sh (Sheep), Eq (Equine), Hu (Human), Bov (Bovine), Gt (Goat), Por (Porcine), Ms (Mouse), Can (Canine), Rt (Rat), Mky (Monkey), Rb (Rabbit), Bab (Baboon)

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Flow Cytometry (F), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Functional assay (FN), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Neutralisation (NEUT)

Background of CD11a / ITGAL antibody

Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling.
ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. 
LFA-1 is part of the family of leukocyte integrins that are recognised by their common β-chains (CD18). LFA-1 also has a distinct α-chain (CD11a). 
LFA-1 is expressed on lymphocytes, monocytes and granulocytes; it has been shown that LFA-1 contributes to the adhesion reaction of these cells. Blocking experiments with anti-LFA monoclonal antibodies demonstrated that LFA-1 inhibits the adhesion step effector and target cells in cytotoxic T lymphocyte, natural killer and lectin dependent cytotoxicity. 
LFA-1 is involved in recruitment to the site of infection. It binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. LFA-1 is the first to bind T-cells to antigen-presenting cells and initially binds weakly. A signal from the T-cell receptor and/or the cytokine receptor changes the conformation and prolongs the cell contact, allowing the T-cell to proliferate.

General readings

1. Sarantos MR, Raychaudhuri S, Lum AF, Staunton DE, Simon SI: Leukocyte function-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1. J Biol Chem. 2005 Aug 5;280(31):28290-8.
2. Giblin PA, Lemieux RM: LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics. Curr Pharm Des. 2006;12(22):2771-95.
3. Kellersch B, Kolanus W: Membrane-proximal signaling events in beta-2 integrin activation. Results Probl Cell Differ. 2006;43:245-57.
4. Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity. Life Sci. 2007 May
30;80(24-25):2320-4.
5. Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
6. Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha). 1990;36(1):41-50.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD11a + CD18 complex antibody

Staining of equine peripheral blood lymphocytes with MOUSE ANTI EQUINE CD11a/CD18, visualised with RABBIT ANTI MOUSE IgG:FITC (STAR9B). Mouse IgG1 CVS9 Supernatant Eq C, F, P
1 ml / €360.00
  Novus Biologicals Inc.

CD11a / ITGAL antibody

CD11a / ITGAL Mouse IgG1 MEM-83 Azide Free Hu F, FN, IP
0.1 mg / €210.00
  Acris Antibodies GmbH

CD11a / ITGAL antibody

CD11a / ITGAL Mouse IgG1 MEM-83 Purified Hu F, FN, IP
0.1 mg / €210.00
  Acris Antibodies GmbH

CD11a / ITGAL antibody

TA305639 (0.5ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD11a / ITGAL antibody

Staining of normal human peripheral blood cells with Anti-Human CD11a FITC. Mouse HI111 Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD11a / ITGAL antibody

Staining of mouse splenocytes with Anti-Mouse CD11a FITC (left) and PE (right).  Appropriate isotype controls were used  (open histogram). Total viable cells were used for analysis. Rat M17/4 Purified Ms F, IP, P
50 µg / €120.00
  OriGene Technologies, Inc.

CD11a / ITGAL antibody

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human esophagus and liver cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus and liver cancer tissue using ITGAL antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD11a / ITGAL antibody

Western blot analysis of extracts of various cell lines, using ITGAL antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD11a / ITGAL (+ CD18) antibody

Staining of equine peripheral blood lymphocytes with CD11a / ITGAL antibody clone CVS9 Cat.-No. AM01043FC-N. Mouse IgG1 CVS9 FITC Eq F
0.1 mg / €450.00
  Acris Antibodies GmbH

CD11a / ITGAL (+ CD18) antibody

Staining of equine peripheral blood lymphocytes with mouse anti horse CD11a/ITGAL antibody clone CVS9 Cat.-No. AM1043PU-S, followed by secondary FITC conjugated antibody. Mouse IgG1 CVS9 Purified Eq C, F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD11a / ITGAL (+ CD18) antibody

CD11a / ITGAL Mouse IgG1 CVS9 PE Eq F
100 Tests / €510.00
  Acris Antibodies GmbH

CD11a / ITGAL (+ CD18) antibody

CD11a / ITGAL Mouse IgG1 CVS9 Supernatant Eq C, ICC/IF
2 ml / €440.00
  Acris Antibodies GmbH

CD11a / ITGAL antibody

CD11a / ITGAL Mouse IgG2a OFYS Biotin Hu F
100 Tests / €270.00
  Acris Antibodies GmbH

CD11a / ITGAL antibody

CD11a / ITGAL Mouse IgG2a OFYS FITC Hu F
100 Tests / €270.00
  Acris Antibodies GmbH

CD11a / ITGAL antibody

CD11a / ITGAL Mouse IgG2a OFYS Purified Hu F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD11a / ITGAL antibody

Figure 1. Peripheral Blood Lymphocytes stained with PE labelled anti-CD11a antibody (AM05207RP-N). Mouse IgG2a OFYS PE Hu F
100 Tests / €300.00
  Acris Antibodies GmbH

CD11a / ITGAL antibody

CD11a / ITGAL Mouse IgG2b BL1H8 FITC Bov, Por, Sh F
0.1 mg / €530.00
  Acris Antibodies GmbH

CD11a / ITGAL antibody

Surface staining of mouse splenocytes using anti-CD11a monoclonal antibody (clone M17/4). Rat IgG2a M17/4 FITC Ms F
0.1 mg / €230.00
  Acris Antibodies GmbH

CD11a / ITGAL antibody

Surface staining of mouse splenocytes using anti-CD11a monoclonal antibody (clone M17/4). Rat IgG2a M17/4 Low Endotoxin Ms C, F, FN, IP
0.1 mg / €200.00
  Acris Antibodies GmbH

CD11a / ITGAL antibody

Surface staining of mouse splenocytes using anti-CD11a monoclonal antibody (clone M17/4). Rat IgG2a M17/4 Aff - Purified Ms C, F, FN, IP
0.1 mg / €160.00
  Acris Antibodies GmbH

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