CD11b antibody

Principal name

CD11b antibody

Alternative names for CD11b antibody

ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, Cell surface glycoprotein MAC-1 subunit alpha, CD11 antigen-like family member B

SwissProt ID

A2TJJ2, P05555 (Mouse), P11215 (Human), P11578 (Cavpo), Q63001 (Rat), Q9TRH2 (Rabit)

Gene ID

3684 (ITGAM), 443217, 16409

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Rb (Rabbit), Mky (Monkey), Bab (Baboon), Bov (Bovine), Can (Canine), Fe (Feline), Gt (Goat), Por (Porcine), Md (Mustelid), Sh (Sheep), Chimp (Chimpanzee)

Available hosts

Mouse, Rat, Rabbit, Goat, Chicken

Available applications

Frozen Sections (C), Flow Cytometry (F), Functional assay (FN), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Neutralisation (NEUT)

Background of CD11b antibody

The CBRM1/5 monoclol antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the α chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.

General readings

1. Springer, T. et al. (1978) Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8:539-551.
2. Springer, T. et al. (1979) Mac-1: a macrophage differentiation antigen identified by a monoclonal antibody. Eur. J. Immunol. 9:301-306.
3. Beller, D.I. et al. (1982) Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor J. Exp. Med. 156:1000-1009.
4. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T cell subsets and other leukocytes in paraffinembedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43:313-320.
5. Klingel, K. et al. (2003) Beta2-microglobulin-associated regulation of interferon-gamma and virus-specific immunoglobulin G confer resistance against the development of chronic coxsackievirus myocarditis. Am. J. Pathol.
162(5):1709-1720.
6. Welt, F.G. et al. (2000) Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries. Arterioscler Thromb Vasc Biol. 20: 2553-2558.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD11b antibody

Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD11b:AZIDE FREE Mouse IgG1 ICRF44 Azide Free Hu, Mky C, F, IP
1 mg / €790.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD11b:FITC Mouse IgG1 ICRF44 FITC Hu, Mky F
0.1 mg / €400.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD11b:FITC Mouse IgG1 ICRF44 FITC Hu, Mky F
25 µg / €190.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD11b (SM1068P). Mouse IgG1 ICRF44 Purified Hu, Mky C, F, FN, IP
0.2 mg / €430.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with Mouse Anti Human CD11b Mouse IgG1 ICRF44 Purified Hu, Mky C, F, FN, IP
0.1 mg / €270.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with Mouse Anti Human CD11b Mouse IgG1 ICRF44 Purified Hu, Mky C, F
20 µg / €180.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with Mouse Anti Human CD11b-RPE Mouse IgG1 ICRF44 PE Hu, Mky F
100 Tests / €470.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with Mouse Anti Human CD11b-RPE Mouse IgG1 ICRF44 PE Hu, Mky F
25 Tests / €220.00
  Acris Antibodies GmbH

CD11b antibody

Western Blot: CD11b Antibody [TA336565] - Detection of CD11b in RAW 264.7 whole cell lysates using TA336565. Rabbit Purified Hu, Ms, Rt C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD11b antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ITGAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807952)(1:150) Mouse IgG1 OTI12C10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD11b antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ITGAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807953)(1:150) Mouse IgG1 OTI2D11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD11b antibody

TA303242 (0.3µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

CD11b antibody

TA303242 (0.3µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD11b antibody

CD11b Mouse CBRM1/5 Purified Hu F, IP
50 µg / €120.00
  OriGene Technologies, Inc.

CD11b antibody

Staining of normal human peripheral blood cells with Anti-Human CD11b PE.  Autofluorescence is indicated by open histogram.  Cells in the monocyte population were used for analysis. Mouse ICRF44 Purified Bab, Chimp, Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD11b antibody

Staining of mouse bone marrow with Anti-Mouse CD11b FITC (left) and PE (right).  Appropriate isotype controls were used  (open histogram).  Cells in the myeloid population were used for anaysis. Rat M1/70 Purified Ms F, IP, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD11b antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human esophagus and cervical cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus and cervical cancer tissue using ITGAM antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD11b antibody

Predicted cell location: Cytoplasm. Positive control: Human gastric and liver cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human gastric and liver cancer tissue using ITGAM antibody at dilution 1/10, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD11b antibody

Western Blot: CD11b/c Antibody [TA336516] - Staining of human bone marrow, myeloid precursors. Rabbit Purified Hu, Ms C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD11b antibody

CD11b Mouse IgG1 BEAR-1 Purified Hu C, F, ICC/IF
0.2 mg / €460.00
  Acris Antibodies GmbH

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