CD11b antibody

Principal name

CD11b antibody

Alternative names for CD11b antibody

ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, Cell surface glycoprotein MAC-1 subunit alpha, CD11 antigen-like family member B

SwissProt ID

A2TJJ2, P05555 (Mouse), P11215 (Human), P11578 (Cavpo), Q63001 (Rat), Q9TRH2 (Rabit)

Gene ID

3684 (ITGAM), 443217, 16409 (Itgam)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Rb (Rabbit), Mky (Monkey), Bov (Bovine), Can (Canine), Fe (Feline), Gt (Goat), Por (Porcine), Md (Mustelid), Sh (Sheep), Chimp (Chimpanzee), Bab (Baboon)

Available hosts

Mouse, Rat, Goat, Rabbit, Chicken

Available applications

Frozen Sections (C), Flow Cytometry (F), Functional assay (FN), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of CD11b antibody

The CBRM1/5 monoclol antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the α chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.

General readings

1. Beller, D.I. et al. (1982) Anti-Mac-1 selectively inhibits the mouse and human type three
complement receptor. J Exp Med. 156 (4): 1000-9.
2. FernándezSuárez, D. (2014) The monoacylglycerol lipase inhibitor JZL184 is neuroprotective
and alters glial cell phenotype in the chronic MPTP mouse model Neurobiol Aging. 35: 2603-16.
3. Welt, F.G. et al. (2000) Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries. Arterioscler Thromb Vasc Biol. 20 (12): 2553-8.
4. Terrando, N. et al. (2010) The impact of IL-1 modulation on the development of lipopolysaccharide-induced cognitive dysfunction. Crit Care. 14 (3): R88.
5. Redensek, A. et al. (2011) Expression and detrimental role of hematopoietic prostaglandin D synthase in spinal cord contusion injury. Glia. 59: 603-14.
6. Brochard, V. et al (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease. J Clin Invest. 119: 182-92.
7. Chinnery, H.R. et al. (2010) Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice.
J Neuropathol Exp Neurol. 69: 896-909.
8. Ferger, A.I. et al (2010) Effects of mitochondrial dysfunction on the immunological properties of microglia. J Neuroinflammation. 7: 45.
9. Gales, A. et al (2010) PPARgamma controls dectin-1 expression required for host antifungal defense against Candida albicans. PLoS Pathog. 6 : e1000714.
10. Geier, H. and Celli, J. (2011) Phagocytic Receptors Dictate Phagosomal Escape and Intracellular Proliferation of Francisella tularensis. Infect Immun. 79: 2204-14.
11. Ghasemlou, N. et al. (2010) Mitogen-activated protein kinase-activated protein kinase 2
(MK2) contributes to secondary damage after spinal cord injury. J Neurosci. 30: 13750-9.
12. Huang, Q.Q. et al (2008) Role of H2-calponin in regulating macrophage motility and phagocytosis.
J Biol Chem. 283: 25887-99.
13. Hudcovic, T. et al (2009) Monocolonization with Bacteroides ovatus protects
immunodeficient SCID mice from mortality in chronic intestinal inflammation caused by
long-lasting dextran sodium sulfate treatment. Physiol Res. 58: 101-10.
14. Kanu, N. et al. (2010) The ATM cofactor ATMIN protects against oxidative stress and accumulation of DNA damage in the aging brain. J Biol Chem. 285: 38534-42.
15. Kapturczak, M.H. et al (2004) Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse. Am J Pathol. 165: 1045-53.
16. Kroner, A. et al (2010) Ectopic T-cell specificity and absence of perforin and granzyme B alleviate neural damage in oligodendrocyte mutant mice. Am J Pathol. 176: 549-55.
17. L'Episcopo, F. et al. (2010) Combining nitric oxide release with anti-inflammatory activity preserves nigrostriatal dopaminergic innervation and prevents motor impairment in a 1-methyl- 4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease. J Neuroinflammation. 7: 83.
18. Samanta, J. et al. (2010) Noggin protects against ischemic brain injury in rodents.
Stroke.41: 357-62.
19. Yang, X. et al (2010) The role of the JAK2-STAT3 pathway in pro-inflammatory responses of
EMF-stimulated N9 microglial cells. J Neuroinflammation. 7: 54.
20. Kondo, Y. et al. (2011) Macrophages counteract demyelination in a mouse model of globoid cell leukodystrophy. J Neurosci. 31: 3610-24.
21. Macrez, R. et al. (2016) Neuroendothelial NMDA receptors as therapeutic targets in
experimental autoimmune encephalomyelitis. Brain. Jul 19. pii: aww172. [Epub ahead of print] 
22. Amantea, D. et al. (2016) Neuroprotective Properties of a Macrolide Antibiotic in a Mouse Model of Middle Cerebral Artery Occlusion: Characterization of the Immunomodulatory Effects and Validation of the Efficacy of Intravenous Administration. Assay Drug Dev Technol. Jul 8. [Epub ahead of print]
23. Werneburg, S. et al. (2016) Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages. Glia. 64 (8): 1314-30.
24. Certo, M. et al. (2015) Activation of RXR/PPARγ underlies neuroprotection by bexarotene in ischemic stroke. Pharmacol Res. 102: 298-307.
25. Chen, Z.Z. et al. (2016) Memantine mediates neuroprotection via regulating neurovascular
unit in a mouse model of focal cerebral ischemia. Life Sci. 150: 8-14.
26. Rich, M.C. et al. (2016) Site-targeted complement inhibition by a complement receptor
2-conjugated inhibitor (mTT30) ameliorates post-injury neuropathology in mouse brains.
Neurosci Lett. 617: 188-94.
27. McCarthy, R.C. et al. (2016) Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta. J Neuroinflammation. 13: 21.
28. Jones, R.S. et al. (2015) Inhibition of JAK2 attenuates the increase in inflammatory markers in microglia from APP/PS1 mice. Neurobiol Aging. 36 (10): 2716-24.
29. Amantea, D. et al. (2016) Azithromycin protects mice against ischemic stroke injury by promoting macrophage transition towards M2 phenotype. Exp Neurol. 275 Pt 1: 116-25.
30. Bains, M. & Roberts, J.L. (2016) Estrogen protects against dopamine neuron toxicity in primary mesencephalic cultures through an indirect P13K/Akt mediated astrocyte pathway.
Neurosci Lett. 610: 79-85.
31. Ji, J. et al. (2015) Iptakalim protects against ischemic injury by improving neurovascular unit function in the mouse brain. Clin Exp Pharmacol Physiol. 42 (7): 766-71.
32. Kim, B.W. et al. (2015) αAsarone attenuates microgliamediated neuroinflammation by inhibiting NF kappa B activation and mitigates MPTP-induced behavioral deficits in a mouse model of Parkinson's disease. Neuropharmacology. 97: 46-57.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD11b antibody

Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD11b:AZIDE FREE Mouse IgG1 ICRF44 Azide Free Hu, Mky C, F, IP
1 mg / €790.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD11b:FITC Mouse IgG1 ICRF44 FITC Hu, Mky F
0.1 mg / €400.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD11b:FITC Mouse IgG1 ICRF44 FITC Hu, Mky F
25 µg / €190.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD11b (SM1068P). Mouse IgG1 ICRF44 Purified Hu, Mky C, F, FN, IP
0.2 mg / €430.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with Mouse Anti Human CD11b Mouse IgG1 ICRF44 Purified Hu, Mky C, F, FN, IP
0.1 mg / €270.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with Mouse Anti Human CD11b Mouse IgG1 ICRF44 Purified Hu, Mky C, F
20 µg / €180.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with Mouse Anti Human CD11b-RPE Mouse IgG1 ICRF44 PE Hu, Mky F
100 Tests / €470.00
  Acris Antibodies GmbH

CD11b antibody

Staining of human peripheral blood granulocytes with Mouse Anti Human CD11b-RPE Mouse IgG1 ICRF44 PE Hu, Mky F
25 Tests / €220.00
  Acris Antibodies GmbH

CD11b antibody

  Rabbit Polyclonal Integrin alpha M/CD11b Antibody  
Western Blot: CD11b Antibody [TA336565] - Detection of CD11b in RAW 264.7 whole cell lysates using TA336565. Rabbit Purified Hu, Ms, Rt C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD11b antibody

  ITGAM mouse monoclonal antibody, clone OTI12C10 (formerly 12C10)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ITGAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807952)(1:150) Mouse IgG1 OTI12C10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD11b antibody

  ITGAM mouse monoclonal antibody, clone OTI2D11 (formerly 2D11)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ITGAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807953)(1:150) Mouse IgG1 OTI2D11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD11b antibody

  Goat Polyclonal Antibody against ITGAM  
TA303242 (0.3µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD11b antibody

  Mouse Anti-Human CD11b (activation epitope) Purified (50 ug)  
CD11b Mouse CBRM1/5 Purified Hu F, IP
50 µg / €120.00
  OriGene Technologies, Inc.

CD11b antibody

  Mouse Anti-Human CD11b Purified (100 ug)  
Staining of normal human peripheral blood cells with Anti-Human CD11b PE.  Autofluorescence is indicated by open histogram.  Cells in the monocyte population were used for analysis. Mouse ICRF44 Purified Bab, Chimp, Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD11b antibody

  Rat Anti-Mouse CD11b Purified (100 ug)  
Staining of mouse bone marrow with Anti-Mouse CD11b FITC (left) and PE (right).  Appropriate isotype controls were used  (open histogram).  Cells in the myeloid population were used for anaysis. Rat M1/70 Purified Ms F, IP, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD11b antibody

  Anti-ITGAM Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human esophagus and cervical cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus and cervical cancer tissue using ITGAM antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD11b antibody

  Anti-ITGAM Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human gastric and liver cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human gastric and liver cancer tissue using ITGAM antibody at dilution 1/10, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD11b antibody

  Rabbit Polyclonal CD11b/c Antibody  
Western Blot: CD11b/c Antibody [TA336516] - Staining of human bone marrow, myeloid precursors. Rabbit Purified Hu, Ms C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD11b antibody

CD11b Mouse IgG1 BEAR-1 Purified Hu C, F, ICC/IF
0.2 mg / €490.00
  Acris Antibodies GmbH

CD11b antibody

CD11b Mouse IgG1 MEM-170 Azide Free Hu F, FN
0.1 mg / €210.00
  Acris Antibodies GmbH

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