CD120a / TNFR1 antibody

Principal name

CD120a / TNFR1 antibody

Alternative names for CD120a / TNFR1 antibody

Tumor necrosis factor receptor 1, TNF-R1, TNF-RI, TNFR-I, p55, p60, Tnfrsf1a, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I

SwissProt ID

O19131 (Bovin), P19438 (Human), P22934 (Rat), P25118 (Mouse), P50555 (Pig)

Gene ID

7132 (TNFRSF1A), 21937 (Tnfrsf1a)

Available reactivities

Bov (Bovine), Can (Canine), Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Sh (Sheep), Gt (Goat), Dros (Drosophila), Xen (Xenopus), Ye (Yeast)

Available hosts

Rabbit, Mouse, Rat, Goat, Hamster

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), Functional assay (FN), Enzyme Immunoassay (E), Immunoprecipitation (IP), Radioimmunoassay (R), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Neutralisation (NEUT), Luminex (LMNX)

Background of CD120a / TNFR1 antibody

TNF RI (CD120a) is a 55 kDa transmembrane glycoprotein that is expressed by virtually all nucleated mammalian cells. Among the numerous cells known to express TNF RI are hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34+ hematopoietic progenitors. Both TNF-alpha and TNF-beta bind to TNF RI. Soluble TNF-alpha binds with a Kda in the range of 20-60 pM, while TNF-beta binds with a Kda equal to 650 pM. TNF RI relative to TNF RII seems to be the more physiologically-relevant receptor, whereas TNF-R2 appears to play a direct role in only a limited number of TNF responses. Soluble TNF RI, which blocks TNF-alpha activity, has been identified in both urine and blood (1-3 ng/mL). Serum levels of sTNF receptors increase dramatically in certain pathological situations. Two soluble forms have been identified and are believed to be generated by proteolytic cleavage. Human TNF RI has 64% amino acid sequence identity (70% in the extracellular region) with mouseTNF-R1, and human TNF RI binds human and mouse TNF-alpha with equal affinity. The extracellular region has four cysteine-rich motifs, the first of which is suggested to be required for binding. The intracellular portion of TNF R1 contains a 'death domain' of about 70 amino acids that is required for the signaling of apoptosis and NF-kappaB activation. TNF binds to the extracellular domain of TNF R1 and induces receptor trimerization. Then, the aggregated death domain of TNF R1 recruits the adapter protein TRADD. TRADD, in turn, recruits FADD, TRAF2 and RIP to form the TNF R1 signaling complex and activate signaling cascades leading to apoptosis, JNK/SAPK activation, and NF-kappaB activation respectively. However, TNF R1 self-associates and signals independently of ligand when overexpressed. This apparent paradox may be explained by silencer of death domains (SODD), a widely expressed approximately 60 kDa protein that was found to be associated with the death domain of TNF-RI.

General readings

1. Thoma, B. et al. (1990) Identification of a 60kDa tumour necrosis factor (TNF) receptor as the major signal transducing component in TNF responses. J. Exp. Med. 172: 1019-1023.
2. Menegazzi, R. et al. (1994) Evidence that tumour necrosis factor alpha (TNF)-induced activation of neutrophil respiratory burst on biologic surfaces is mediated by the p55 TNF receptor. Blood 84: 287-293.
3. Dri, P. et al. (1999) Role of the 75-kDa TNF receptor in TNF-induced activation of neutrophil respiratory burst. J. Immunol. 162: 460-466.
4. Kohrgruber, N. et al. (1999) Survival, maturation, and function of CD11c- and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines.
J Immunol. 163:3250-3259.
5. Weigart, N. et al. (1996) Gastrin secretion from primary cultures of rabbit antral G cells; stimulation by inflammatory cytokines. Gasteroenterology. 110: 147 - 154.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD120a / TNFR1 antibody

(0.3ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD120a / TNFR1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TNF receptor-1 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD120a / TNFR1 antibody

CD120a / TNFR1 Rabbit Purified Bov, Can, Hu, Mky, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD120a / TNFR1 antibody

Western blot analysis of extracts of HeLas cell and HL60 cell lines, using TNFR1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD120a / TNFR1 antibody

To detect hsTNF-Receptor I by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTNF-Receptor I. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD120a / TNFR1 antibody

To detect hsTNF-receptor by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTNF-receptor (TA328457) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD120a / TNFR1 antibody

WB Suggested Anti-TNFRSF1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: DU145 cell lysateTNFRSF1A is supported by BioGPS gene expression data to be expressed in DU145 Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD120a / TNFR1 antibody

TNFRSF1A ELISA with TBP-1Capture (TA600021) and H398 Detection (TA700021) Antibodies. Substrate used: Recombinant Human TNFRSF1A (TP301284) Mouse IgG1 OTITBP-1 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD120a / TNFR1 antibody

TNFRSF1A ELISA with TBP-1Capture (TA600021) and H398 Detection (TA700021) Antibodies. Substrate used: Recombinant Human TNFRSF1A (TP301284) Mouse IgG1 OTIH398 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

CD120a / TNFR1 antibody

CD120a / TNFR1 Mouse IgG1 MR1-2 Biotin Hu, Mky C, E
50 µg / €360.00
  Acris Antibodies GmbH

CD120a / TNFR1 antibody

CD120a / TNFR1 Mouse IgG1 MR1-2 Purified Hu, Mky C, E, FN
0.1 mg / €440.00
  Acris Antibodies GmbH

CD120a / TNFR1 antibody

CD120a / TNFR1 Hamster IgG 55R-170 FITC Ms E, FN, IP
0.1 mg / €390.00
  Acris Antibodies GmbH

CD120a / TNFR1 antibody

CD120a / TNFR1 Hamster IgG 55R-170 Purified Ms E, FN, IP
0.1 mg / €340.00
  Acris Antibodies GmbH

CD120a / TNFR1 antibody

CD120a / TNFR1 Mouse MR1-2 Biotin Hu C, E, IP, WB
0.5 ml / €370.00
  Acris Antibodies GmbH

CD120a / TNFR1 antibody

CD120a / TNFR1 Mouse IgG1 MR1-2 Purified Hu C, E, IP, WB
1 ml / €370.00
  Acris Antibodies GmbH

CD120a / TNFR1 Control Peptide

CD120a / TNFR1 Purified
50 µg / €190.00
  Acris Antibodies GmbH

CD120a / TNFR1 antibody

Western blot analysis of TNF-R1 expression in rat brain tissue lysate. Rabbit Aff - Purified Bov, Can, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

CD120a / TNFR1 antibody

Western blot analysis of TNF-R1 antibody (Cat.-No AP06465PU-N) at 1/500 dilution. 
Lane 1: Hela whole cell lysate treated with PMA.
Lane 2: Mouse liver tissue lysate.
Lane 3: Rat liver tissue lysate. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD120a / TNFR1 antibody

Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bov, Hst, Hu, Mky, Ms, Por, Rb, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD120a / TNFR1 pSer274 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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