CD120b / TNFR2 antibody

Principal name

CD120b / TNFR2 antibody

Alternative names for CD120b / TNFR2 antibody

Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, TNFRSF1B, TNFBR, TNF-R2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II

SwissProt ID

P20333 (Human), P25119 (Mouse), Q80WY6 (Rat)

Gene ID

7133 (TNFRSF1B), 21938 (Tnfrsf1b), 7186 (TRAF2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit, Goat, Rat, Hamster, Chicken

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Functional assay (FN), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of CD120b / TNFR2 antibody

Tumor necrosis factor alpha (TNF-alpha ; TNF alpha) and the structurally related lymphotoxin-alpha (LT-a) are pro-inflammatory cytokines produced by a variety of immune and non-immune cells in response to multiple stimuli. These stimuli can include bacteria and bacterial products such as LPS, viral infections, T-cell receptor activation and cross-linking of surface immunoglobulin (Ig) on B cells. Expression of TNF-a has been detected in macrophages/monocytes, B-cells, T-cells, basophils, eosinophils, glial cells and mast cells. The effects of TNF-alpha and LT-a are mediated through the TNF receptors, TNF RI and TNF RII. These receptors are type I transmembrane glycoproteins belonging to the TNF receptor superfamily, which is defined by the presence of repeating units of cysteine clusters. TNF RI is present on virtually all cell types except for red blood cells. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Clone 80M2 is used in this references:
1. Grell, M., et al. Lymphokine Cytokine Res. 12, 143-148 (1993)
2. Grell, M., et al. Cell 83, 793-802 (1995).

  1. Quick-Filters:
  2. Overnight Delivery (1)

Items 1 to 20 of 86 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD120b / TNFR2 antibody

Human liver carcinoma stained with anti-TNF-R2 antibody Rabbit Purified Hu, Ms, Rt IP, P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

Human liver carcinoma stained with anti-TNF-R2 antibody Rabbit Purified Hu, Ms, Rt IP, P, WB
1 ml / €440.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

Human liver carcinoma stained with anti-TNF-R2 antibody Rabbit Purified Hu, Ms, Rt IP, P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

(0.2ug/ml) staining of Rat Spinal Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD120b / TNFR2 antibody

Western blot analysis of extracts from RAW264.7 cells, HT-29 cells and LOVO cells, using TNF Receptor II antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD120b / TNFR2 antibody

Predicted band size: 48 kDa . Positive control: Human colon cancer lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD120b / TNFR2 antibody

Predicted band size: 48 kDa . Positive control: Human colon cancer lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD120b / TNFR2 antibody

Western blot analysis of 293 cell and MCF7 cell lysate using TNFRSF1B antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD120b / TNFR2 antibody

This antibody stained PC-3 and CaCo-2 cells. The primary antibody was incubated at 3.0 mg/ml overnight at 4C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information is courtesy of the Cell Profiling group, SciLifeLab Stockholm. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD120b / TNFR2 antibody

Western blot analysis on LOVO cell lysate using TNF-R2 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD120b / TNFR2 antibody

Western blot analysis on LOVO cell lysate using TNF-R2 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD120b / TNFR2 antibody

CD120b / TNFR2 Mouse IgG2a 2/220 Purified Hu C, E, FN, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

CD120b / TNFR2 Mouse IgG1 80M2 Azide Free Hu F
0.1 mg / €340.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

CD120b / TNFR2 Mouse IgG1 80M2 FITC Hu F
50 Tests / €300.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

CD120b / TNFR2 Mouse IgG2a KT30 Aff - Purified Hu E
1 mg / €910.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

CD120b / TNFR2 Mouse IgG2a KT30 Aff - Purified Hu E
0.2 mg / €450.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

CD120b / TNFR2 Mouse IgG2a KT30 Aff - Purified Hu E
0.1 mg / €340.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

CD120b / TNFR2 Mouse IgG1 80M2 Biotin Hu, Rt E, FN, ICC/IF, IP
50 µg / €360.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

CD120b / TNFR2 Mouse IgG1 80M2 FITC Hu, Rt E, F, FN, ICC/IF, IP
0.1 mg / €540.00
  Acris Antibodies GmbH

CD120b / TNFR2 antibody

CD120b / TNFR2 Mouse IgG1 80M2 Purified Hu, Rt E, F, FN, ICC/IF, IP
0.1 mg / €440.00
  Acris Antibodies GmbH

Items 1 to 20 of 86 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  • LinkedIn