CD122 / IL2RB antibody

Principal name

CD122 / IL2RB antibody

Alternative names for CD122 / IL2RB antibody

Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL2 Receptor beta, P70-75

SwissProt ID

P14784 (Human), P16297 (Mouse), P26896 (Rat)

Gene ID

3560 (IL2RB), 16185 (Il2rb), 25746

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Neutralisation (NEUT), Functional assay (FN), Frozen Sections (C)

Background of CD122 / IL2RB antibody

Interleukin-2 (IL-2) is a pleiotropic glycoprotein that mediates the production of regulatory T cells and promotes T cell dependent tolerance. Additionally, IL-2 promotes the proliferation of antigen-activated T lymphocytes, facilitates the cytolytic activity of natural killer (NK) cells and stimulates anti-tumor activity in monocytes by inducing synthesis of GM-CSF, IL-1b and IL-6. IL-2 also promotes the proliferation of large granular lymphocytes and the growth and differentiation of mitogen activated B lymphocytes in vitro. The biological effects of IL-2 are mediated through the IL-2 receptor, which is predominantly expressed on activated T cells, B cells and monocytes. There are three subunits that may associate in different combinations to form the IL-2 receptor. These subunits include IL-2Ra, IL-2 R beta (IL-2Rb), which is shared with IL-15 receptor and IL-2Rg, which is also known as the common cytokine receptor gamma chain (gc) as it is shared with receptors for IL-4, IL-7, IL-9, IL-15 and IL-21. Different associations of these subunits form low affinity, intermediate affinity or high affinity IL-2 receptors. The low affinity receptor comprises the IL-2Ra glycopeptide, the intermediate affinity receptor is formed by the association of IL-2R beta and the gc while the high affinity receptor comprises IL-2 Ra, IL-2 Rb and gc chains. IL-2 R beta is expressed on resting T cells and is up regulated by T cell activation, particularly by treatment with IL2, anti-CD3 and anti CD28. The beta subunit is involved in both binding to IL2 and mediating signal activation. IL-2 R beta is a glycoprotein that contains 4 potential N-linked glycosylation sites. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD122 / IL2RB antibody

Western blot analysis of extracts from COS7 cells, using IL-2Rß/CD122 (Ab-364) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD122 / IL2RB antibody

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1.1 APC and 0.25 ug of Rat IgG2b K Isotype Control Purified (left) or 0.5 ug of Anti-Mouse CD122 Purified (right) followed by Anti-Rat IgG Biotin and Streptavidin PE .  Cells in the lymphocyte gate were used for analysis. Rat TM-b1 (TM-beta1) Purified Ms F
0.1 mg / €130.00
  OriGene Technologies, Inc.

CD122 / IL2RB antibody

Western blot analysis on HeLa cell lysate using IL-2Rbeta/CD122 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD122 / IL2RB antibody

CD122 / IL2RB Rat IgG2a 5H4 APC Ms F
0.1 mg / €350.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

CD122 / IL2RB Rat IgG2a 5H4 Biotin Ms F
0.5 mg / €330.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

Immunofluorescent Staining: BALB/c lymph node cells were double stained with Rat anti-Mouse CD3e-PE (Cat#AM08026RP-N or AM08026RP-S) and Rat anti-Mouse CD122-FITC. Small lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells. Rat IgG2a 5H4 FITC Ms F
0.5 mg / €350.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

CD122 / IL2RB Rat IgG2a 5H4 Low Endotoxin Ms F, IP
0.5 mg / €350.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

CD122 / IL2RB Rat IgG2a 5H4 Purified Ms F, IP
0.5 mg / €290.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

CD122 / IL2RB Rat IgG2a 5H4 PE Ms F
0.1 mg / €270.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

Immunohistochemistry analyzes of IL-2Rβ antibody (AP20249PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

Western blot analysis of IL-2Rβ antibody (Cat.-No.: AP20310PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

Western blot analysis of IL-2Rβ antibody (Cat.-No.: AP20615PU-N) in extracts from RAW264.7 cells. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD122 / IL2RB pTyr364 antibody

Western blot analysis of extracts from COS7 cells using IL-2Rβ/CD122 (Phospho-Tyr364) Antibody AP55726PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

CD122 / IL2RB pTyr364 antibody

Western blot analysis of extracts from COS7 cells using IL-2Rβ/CD122 (Phospho-Tyr364) Antibody AP55726PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

CD122 / IL2RB (Extracell. Dom.) antibody

CD122 / IL2RB Rat IgG2 9E33 Purified Ms WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

CD122 / IL2RB Mouse IgG1 L316 FITC Rt F
0.1 mg / €430.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

CD122 / IL2RB Mouse IgG1 L316 PE Rt F
100 Tests / €430.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

CD122 / IL2RB Rat IgG2b TM-BETA1 FITC Ms F
0.1 mg / €330.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

CD122 / IL2RB Rat IgG2b TM-BETA1 Low Endotoxin Ms F, FN, IP
0.5 mg / €560.00
  Acris Antibodies GmbH

CD122 / IL2RB antibody

CD122 / IL2RB Rat IgG2b TM-BETA1 Purified Ms F, IP
0.25 mg / €400.00
  Acris Antibodies GmbH

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