CD123 / IL3RA antibody

Principal name

CD123 / IL3RA antibody

Alternative names for CD123 / IL3RA antibody

Interleukin-3 receptor subunit alpha, IL-3R-alpha, IL3 receptor alpha

SwissProt ID

P26951 (Human), P26952 (Mouse)

Gene ID

3563 (IL3RA), 16188 (Il3ra)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine)

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Neutralisation (NEUT)

Background of CD123 / IL3RA antibody

IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The α subunit (CD123) specifically binds IL3, whereas the b subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment. (Bendriss-Vermare N thesis, 2001;Treilleux I et al, 2004 ; Clin. Canc. Res., 10: 7466-7474).

General readings

Clone 6H6 is used in these references:
1. Herbeuval, J-P., et al., PNAS 103, 7000-7005 (2006)
2. Lan, P., et al., Blood 108, 487-492 (2006)
3. Gregory, B., et al., J. Immunol. 170, 5359-5366 (2003)
4. Herling, M., et al., Blood 101, 5007-5009 (2003)
5. Kempuraj, D., et al., Blood 93, 3338-3346 (1999)
6. Jenkins, B. J., et al., J. Biol. Chem. 271, 29707-29714 (1996)
7. Rapoport, A. P., et al., Blood 87, 112-122 (1996)
8. Sun, Q., et al. Blood 87, 83-92 (1996)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD123 / IL3RA antibody

CD123 / IL3RA Mouse IgG1 6H6 Purified Hu C, F, IP, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD123 / IL3RA antibody

Staining of TF-1 cell line with Mouse IgG1 kappa Isotype Control Purified (open histogram) or Anti-Human CD123 Purified (filled histogram) followed by F(ab')2 Anti-Mouse IgG PE .  Total viable cells were used for analysis. Mouse 6H6 Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD123 / IL3RA antibody

IL3RA Antibody (C-term) (Cat. #TA325019) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the IL3RA antibody detected the IL3RA protein (arrow). Rabbit IgG Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CD123 / IL3RA antibody

Host: Rabbit; Target Name: IL3RA; Sample Tissue: THP-1 whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD123 / IL3RA antibody

TA348953 (2 ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD123 / IL3RA antibody

CD123 / IL3RA Rat IgG2a 5B11 Purified Ms F
25 µg / €170.00
  Acris Antibodies GmbH

CD123 / IL3RA (Extracell. Dom.) antibody

CD123 / IL3RA Rat IgG2 9G52 Purified Ms WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CD123 / IL3RA antibody

CD123 / IL3RA Mouse IgG1 6H6 APC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD123 / IL3RA antibody

CD123 / IL3RA Mouse IgG1 6H6 Biotin Hu F
0.1 mg / €260.00
  Acris Antibodies GmbH

CD123 / IL3RA antibody

Figure 1. Flow Cytometric analysis of CD123/IL-3R expression on IL-3R transfected CHO cells (A) and the parental CHO cells (B). 
Open Histogram indicates the reaction of isotypic
control to the cells. 
Shaded Histogram indicates the reaction of CD123 antibody to the cells. Mouse IgG1 6H6 FITC Hu F
50 µg / €350.00
  Acris Antibodies GmbH

CD123 / IL3RA antibody

Figure 1. Flow Cytometric analysis of CD123/IL-3R alpha/beta/gamma expression on IL-3R transfected CHO cells (Right) and CHO cells (Left). Open Histogram indicates the reaction of isotypic Control to the cells. Shaded Histogram indicates the reaction of CD123 antibody to the cells. Mouse IgG1 6H6 PE Hu F
1 ml (50 Tests) / €350.00
  Acris Antibodies GmbH

CD123 / IL3RA antibody

CD123 / IL3RA Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CD123 / IL3RA antibody

Western Blot analysis of IL3RA expression in transfected 293T cell line (H00003563-T01) by IL3RA MaxPab polyclonal antibody.

Lane 1: IL3RA transfected lysate(43.30 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD123 / IL3RA antibody

CD123 / IL3RA Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

CD123 / IL3RA antibody

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD123 Antibody-FITC labled. Mouse IgG1 FITC Hu F, ICC/IF
50 Assays / €250.00
  Cohesion Biosciences Ltd.

CD123 / IL3RA antibody

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD123 Antibody-FITC labled. Mouse IgG1 FITC Hu F, ICC/IF
100 Assays / €330.00
  Cohesion Biosciences Ltd.

CD123 / IL3RA antibody

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD123 Antibody-PE labled. Mouse IgG1 PE Hu F, ICC/IF
50 Assays / €280.00
  Cohesion Biosciences Ltd.

CD123 / IL3RA antibody

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD123 Antibody-PE labled. Mouse IgG1 PE Hu F, ICC/IF
100 Assays / €420.00
  Cohesion Biosciences Ltd.

CD123 / IL3RA antibody

Flow cytometric analysis of human peripheral blood cells using Anti-CD123 Antibody; followed by anti-mouse IgG PE. Mouse IgG1 Aff - Purified Hu F, ICC/IF
50 µl / €230.00
  Cohesion Biosciences Ltd.

CD123 / IL3RA antibody

Flow cytometric analysis of human peripheral blood cells using Anti-CD123 Antibody; followed by anti-mouse IgG PE. Mouse IgG1 Aff - Purified Hu F, ICC/IF
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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