CD125 / IL5RA antibody

Principal name

CD125 / IL5RA antibody

Alternative names for CD125 / IL5RA antibody

Interleukin-5 receptor subunit alpha, IL-5R-alpha, IL5 Receptor alpha

SwissProt ID

P21183 (Mouse), Q01344 (Human)

Gene ID

3568 (IL5RA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of CD125 / IL5RA antibody

Interleukin 5 (IL5), produced primarily by activated T lymphocytes and mast cells, has diverse biological effects on a variety of cell types. IL5 is a potent eosinophil differentiation and activation factor in vivo and in vitro. It also stimulates the proliferation and/or differentiation of basophils and B cells. The effects of IL5 are mediated by binding of the cytokine to specific cell surface receptors expressed on target cells. The high-affinity receptor complex for IL5 consists of a cytokine specific ligand binding alpha chain and a signaling beta chain that can modulate the ligand binding affinity of the receptor complex. The beta chain is shared with the high affinity receptor complexes for IL3 and GMCSF. Alone, the beta chain does not bind cytokines but is needed for cytokine-mediated signaling. Human and mouse cDNA clones for the alpha chain of the high affinity IL5 receptor (IL5RA) complexes have been isolated. The 315 amino acid residue recombinant human IL5RA has a predicted molecular mass of approximately 38 kDa. Due to glycosylation, the recombinant protein migrates as a 43 kDa band in SDS-PAGE. Human and mouse IL5RA are members of the hematopoietin receptor superfamily characterized by the presence of the WSXWS, and a four cysteine residue motif in the extracellular domain of the transmembrane protein. In addition to the cDNA clone encoding the full-length transmembrane protein, cDNA clones (arising from alternative splicing and encoding soluble secreted forms of IL5RA) have been isolated from mouse as well as human cells. A naturally occurring soluble form of the IL5RA has been detected in biological fluids of autoimmune-prone mice and mice bearing chronic B cell leukemia. Recombinant human IL5RA binds human IL5 in a 1:1 ratio and acts as a human IL5 antagonist. The molecule inhibits the proliferation of IL5 dependent cell lines and blocks human umbilical cord blood eosinophil differentiation.

General readings

Bhattacharya M., et al. (2007) Biopolymers. 88:83-93.Emslie D., et al. (2008) J Exp Med. 205:409-421.

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD125 / IL5RA antibody

Immunohistochemistry analysis of  CD125 / IL5RA Antibody Cat.-No. AP20672PU-N in Paraffin-Embedded Human pancreas tissue. Rabbit Aff - Purified Hu, Ms P
0.1 mg / €350.00
  Acris Antibodies GmbH

CD125 / IL5RA antibody

Western Blot analysis of IL5RA expression in transfected 293T cell line (H00003568-T01) by IL5RA MaxPab polyclonal antibody.

Lane 1: IL5RA transfected lysate(38.00 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD125 / IL5RA antibody

CD125 / IL5RA Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

CD125 / IL5RA antibody

CD125 / IL5RA Rabbit Hu E, WB
50 µg / €480.00
  Novus Biologicals Inc.

CD125 / IL5RA antibody

Immunohistochemistry-Paraffin: IL5RA Antibody [NBP1-51248] - analyzes of IL-5Ra (K68) pAb in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms P
0.1 mg / €420.00
  Novus Biologicals Inc.

CD125 / IL5RA antibody

Immunohistochemistry-Paraffin: IL5RA Antibody [NBP1-86548] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD125 / IL5RA (transcript variant 3)

CD125 / IL5RA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD125 / IL5RA (transcript variant 5)

CD125 / IL5RA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD125 / IL5RA (18-339, His-tag)

CD125 / IL5RA Mouse Purified > 90 % by SDS - PAGE
0.25 mg / €940.00
  Acris Antibodies GmbH

CD125 / IL5RA (18-339, His-tag)

CD125 / IL5RA Mouse Purified > 90 % by SDS - PAGE
50 µg / €300.00
  Acris Antibodies GmbH

CD125 / IL5RA

CD125 / IL5RA Human
10 µg / €540.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

IL5RA overexpression lysate

IL5RA overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

IL5RA overexpression lysate

IL5RA overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

IL5RA overexpression lysate

IL5RA overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

per page
  • LinkedIn