CD131 / CSF2RB antibody
Principal name
CD131 / CSF2RB antibody
Alternative names for CD131 / CSF2RB antibody
IL3RB, IL5RB, GM-CSF-RB, Cytokine receptor common subunit beta, GM-CSF/IL-3/IL-5 receptor common beta-chain
Gene ID
1439 (CSF2RB)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunoprecipitation (IP)
Background of CD131 / CSF2RB antibody
Interleukin 3 (IL3), originally called Colony Forming Unit-Stimulating Activity (CFU-SA) is a multifunctional cytokine produced by activated T lymphocytes or mast cells. IL3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. It induces colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar suspended bone marrow cells. Interleukin 3 binds specifically and with high affinity to cell-surface receptors. Interleukin 3 receptors are members of the cytokine receptor superfamily. The IL3 receptor complex contains two distinct cytokine receptor subunits, ligand binding IL3 Ralpha and IL3 Rbeta. Recombinant human IL3 sRalpha (286 amino acid residue) has a predicted molecular mass of approximately 33 kDa. The alpha subunit alone binds IL3 with low affinity. The beta subunit does not bind IL3 by itself but is required for the high-affinity binding of IL3 to the heterodimeric receptor complex. The alpha subunit is also a component of the high-affinity receptor complex for IL5 and GMCSF.
General readings
Hansen G., et al. (2008) Cell. 134:496-507.Mulhern TD., et al. (2000) J Mol Biol. 297:989-1001.
Western blot analysis of IL-3R Antibody (Cat.-No AP06180PU-N) in extracts from RAW264.7 cells treated with G-CSF 25 ng/ml 15'.
Immunohistochemistry analysis of IL-3R Antibody (Cat.-No AP06180PU-N) in paraffin-embedded human brain tissue.
Western blot analysis of CSF2RB polyclonal antibody ( Cat # PAB4461 ) in HL-60 cell line lysates ( 35 µg/lane ) . CSF2RB ( arrow ) was detected using the purified CSF2RB polyclonal antibody ( Cat # PAB4461 ) ( 1 : 60 dilution ) .
(LEFT) The anti-Phospho-IL3R-Y766 Pab (Cat.#AP12630PU-N) is used in Western blot to detect Phospho-IL3R-Y766 in mouse heart tissue lysate. (RIGHT) Dot blot analysis of anti-hIL3R-pY766 Phospho-specific Pab (Cat.#AP12630PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Western blot analysis of anti-IL3R Pab in HL60 cell line lysates (35ug/lane). IL3R (arrow) was detected using the purified Pab (1:60 dilution).
Western Blot analysis of CSF2RB expression in transfected 293T cell line (H00001439-T01) by CSF2RB MaxPab polyclonal antibody.Lane 1: CSF2RB transfected lysate(96.20 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of CD131 expression in HL60 (A); TF1 (B); SP2/0 (C); H9C2 (D) whole cell lysates.
Western blot analysis of CD131 (pY593) expression in MCF7 IL1b-treated (A); HepG2 LPS-treated (B) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2RB (RC218581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2RB.(1:2000)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CSF2RB mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807873)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2RB (RC218581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2RB.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2RB (RC218581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2RB.(1:2000)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-CSF2RB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807875)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2RB (RC218581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2RB.(1:2000)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CSF2RB mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807896)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CSF2RB mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807896)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2RB (RC218581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2RB.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CSF2RB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807897)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CSF2RB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807897)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CSF2RB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807897)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2RB (RC218581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2RB.(1:2000)
CD131 transfected cell stained with purified 1C1, followed by anti-mouse IgG FITC
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA807897 | CD131 / CSF2RB antibody |
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| CSF2RB mouse monoclonal antibody, clone OTI4H3 (formerly 4H3) | |||||||
 |
Mouse | IgG1 | OTI4H3 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| AP06180PU-N | CD131 / CSF2RB antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€350.00
|
||
| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA320316 | CD131 / CSF2RB antibody |
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| Mouse Anti-Human CD131 Purified (100 ug) | |||||||
 |
Mouse | 1C1 | Purified | Hu | F, IP, WB |
0.1 mg /
€120.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA352570 | CD131 / CSF2RB antibody |
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| Mouse Monoclonal anti-CDw131 Antibody | |||||||
 |
Mouse | 1C1 | Purified | Hu | F, IP, WB |
0.1 mg /
€248.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA807872 | CD131 / CSF2RB antibody |
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| CSF2RB mouse monoclonal antibody, clone OTI2B10 (formerly 2B10) | |||||||
 |
Mouse | IgG1 | OTI2B10 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807873 | CD131 / CSF2RB antibody |
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| CSF2RB mouse monoclonal antibody, clone OTI7G6 (formerly 7G6) | |||||||
 |
Mouse | IgG2b | OTI7G6 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA807874 | CD131 / CSF2RB antibody |
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| CSF2RB mouse monoclonal antibody, clone OTI1A6 (formerly 1A6) | |||||||
 |
Mouse | IgG2b | OTI1A6 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA807875 | CD131 / CSF2RB antibody |
||||||
| CSF2RB mouse monoclonal antibody, clone OTI1G5 (formerly 1G5) | |||||||
 |
Mouse | IgG1 | OTI1G5 | Purified | Hu | P |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807876 | CD131 / CSF2RB antibody |
||||||
| CSF2RB mouse monoclonal antibody, clone OTI2G6 (formerly 2G6) | |||||||
 |
Mouse | IgG2a | OTI2G6 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807896 | CD131 / CSF2RB antibody |
||||||
| CSF2RB mouse monoclonal antibody, clone OTI7A4 (formerly 7A4) | |||||||
 |
Mouse | IgG2a | OTI7A4 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP12630PU-N | CD131 / CSF2RB pTyr766 antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| AP13446PU-N | CD131 / CSF2RB (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| DM3566P | CD131 / CSF2RB (Extracell. Dom.) antibody |
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|
Rat | IgG2 | 42A7 | Purified | Ms | WB |
0.1 mg /
€440.00
|
| Acris Antibodies GmbH | |||||||
| A0444-1 | CD131 / CSF2RB pTyr593 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µg /
€230.00
|
||
| Assay Biotech | |||||||
| +1 additional image |
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| A0444-2 | CD131 / CSF2RB pTyr593 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€330.00
|
||
| Assay Biotech | |||||||
| +1 additional image |
|||||||
| B0444-1 | CD131 / CSF2RB antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µg /
€230.00
|
||
| Assay Biotech | |||||||
| +1 additional image |
|||||||
| B0444-2 | CD131 / CSF2RB antibody |
||||||
|
Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€330.00
|
||
| Assay Biotech | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN218581 | CSF2RB - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN218581BN | CSF2RB - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN218581LP | CSF2RB - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
