CD135 / FLT3 antibody

Principal name

CD135 / FLT3 antibody

Alternative names for CD135 / FLT3 antibody

FL cytokine receptor, STK1, Tyrosine-protein kinase receptor FLT3, Stem cell tyrosine kinase 1

SwissProt ID

P36888 (Human), Q00342 (Mouse)

Gene ID

2322 (FLT3), 14255 (Flt3)

Available reactivities

Hst (Hamster), Hu (Human), Mky (Monkey), Prim (Primate), Rb (Rabbit), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP)

Background of CD135 / FLT3 antibody

CD135 is a tyrosine kinase receptor expressed on normal cells including CD34+ hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. CD135 is also expressed on malignant hematopoietic cells including AML, ALL and CML BC. CD135, also known as FMS-like tyrosine kinase 3, FLT3, STK1, and Flk2, is a growth factor receptor that binds the FLT3 ligand to promote the growth and differentiation of primitive hematopoietic cells. The intracytoplasmic domain of CD135 is modified by phosphorylation and has been shown to interact with Grb2, SOCS1, VAV1, and Shc. In humans, expression of Flt3 is restricted to subsets of CD34 positive as well as CD34 negative normal bone marrow cells. In these cells, the level of expression of Flt3 is rather low. Most of the CD34 bright Flt3+ cells co-express CD117 at high levels. They may represent early cycling, but not quiescent stem cells. Flt3+ cells in the CD34lo and CD34- populations do not co-express CD117 molecule and may represent B lymphoid precursors.

General readings

Rosnet O, Schiff C, Pebusque MJ, et al. Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells. Blood 1993; 82:1110-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD135 / FLT3 antibody

  Rabbit anti-FLT3 polyclonal antibody  
Immunofluorescence staining of methanol-fixed HeLa cells using FLT3 antibody (red). Rabbit IgG Purified Hu ICC/IF
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD135 / FLT3 (N-term) antibody

  Rabbit Polyclonal FLT3 Antibody  
Western blot analysis of FLT3 in 3T3 cell lysate with FLT3 antibody at (A) 1 and (B)2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD135 / FLT3 antibody

  Rat Anti-Mouse CD135 (Flt3) Purified (100 ug)  
CD135 / FLT3 Rat A2F10 Purified Ms F, IP
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD135 / FLT3 antibody

  Rat Anti-Mouse VEGF Receptor 3 Purified (50 ug)  
CD135 / FLT3 Rat AFL4 Purified Ms F, IP, P, WB
50 µg / €120.00
  OriGene Technologies, Inc.

CD135 / FLT3 antibody

  Mouse Anti-Human CD135 (Flt3) Purified (25 ug)  
CD135 / FLT3 Mouse BV10A4H2 Purified Hu F
25 µg / €120.00
  OriGene Technologies, Inc.

CD135 / FLT3 (N-term) antibody

  Rabbit polyclonal FLT3 (CD135) Antibody (N-term)  
Western blot analysis of anti-FLT3 Pab (Cat. #TA324962) in HL-60 cell lysate. FLT3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CD135 / FLT3 antibody

  Rabbit Polyclonal Anti-FLT3 Antibody (N-Terminus)  
Anti-FLT3 / CD135 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu, Mky
50 µg / €400.00
  OriGene Technologies, Inc.

CD135 / FLT3 antibody

  Rabbit Polyclonal Anti-FLT3 Antibody  
CD135 / FLT3 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD135 / FLT3 antibody

  FLT3 mouse monoclonal antibody, clone OTI7D6 (formerly 7D6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FLT3 (RC211459, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FLT3.(1:500) Mouse IgG1 OTI7D6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD135 / FLT3 antibody

CD135 / FLT3 Mouse IgG1 BV10A4 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD135 / FLT3 antibody

CD135 / FLT3 Mouse IgG1 BV10A4 Aff - Purified Hu F, IP

Regular Price: 0.1 mg / €210.00

Special Price: 0.1 mg / €133.00

  Acris Antibodies GmbH

CD135 / FLT3 antibody

CD135 / FLT3 Mouse IgG1 BV10A4 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD135 / FLT3 antibody

Western blot (WB) analysis of Flt3 antibody (Cat.-No.: AP01277PU-N) in extracts from MCF-7 cells. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD135 / FLT3 (Internal) antibody

Interfollicular Zone: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

CD135 / FLT3 antibody

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using AP09468PU FLT3 Antibody (Red). Rabbit Aff - Purified Hu ICC/IF, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

CD135 / FLT3 antibody

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using AP09468PU FLT3 Antibody (Red). Rabbit Aff - Purified Hu ICC/IF
50 µl / €240.00
  Acris Antibodies GmbH

CD135 / FLT3 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD135 / FLT3 pTyr591 antibody

Immunohistochemistry (IHC) analyzes of p-FLT3 antibody (Cat.-No.: AP20941PU-N) in paraffin-embedded human lung adenocarcinoma tissue. Rabbit Aff - Purified Hu P
0.1 mg / €350.00
  Acris Antibodies GmbH

CD135 / FLT3 antibody

Immunohistochemistry (IHC) analyzes of FLT3 antibody (Cat.-No.: AP21030PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu P
0.1 mg / €350.00
  Acris Antibodies GmbH

CD135 / FLT3 pTyr842 antibody

Western blot analysis of extracts from HepG2 cells treated with EGF using FLT3 (Phospho-Tyr842)) Antibody AP55755PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

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