CD14 antibody

Principal name

CD14 antibody

Alternative names for CD14 antibody

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

SwissProt ID

P08571 (Human), P10810 (Mouse), Q28680 (Rabit), Q63691 (Rat), Q95122 (Bovin)

Gene ID

929 (CD14), 12475 (Cd14), 509290 (PPP2R2B), 604 (BCL6), 281048

Available reactivities

Hu (Human), Prim (Primate), Bov (Bovine), Can (Canine), Fe (Feline), Gt (Goat), Mink, Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ll (Llama), Bab (Baboon), Eq (Equine), Md (Mustelid), Ms (Mouse), Fi (Fish), Trout, Rt (Rat)

Available hosts

Mouse, Chicken, Goat, Rabbit, Rat

Available applications

Frozen Sections (C), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Functional assay (FN), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Neutralisation (NEUT)

Background of CD14 antibody

The CD14 receptor is a pattern recognition molecule in the innate immune response against microorganisms and other exogenous and endogenous stress factors. CD14 was characterized as a receptor for LPS. The CD14 gene consists of two exons which code for a single mRNA that is translated into a protein of 375 amino acids. The CD14 protein is composed of eleven leucin-rich repeats, which are also found in TLR and which are important in PAMP binding. In contrast to TLR, however, CD14 lacks a transmembrane domain, and thus cannot initiate intracellular signal transduction by itself. The most important CD14 signaling co-receptor is toll-like receptor 4 (TLR4), which activates, among others, the nuclear factor κB (NF-κB) inflammatory pathway. The CD14 protein is processed in the endoplasmatic reticulum and expressed as a 55 kDa glycoprotein on the cell surface via a glycosylphosphatidyl (GPI) anchor. Like other GPI-anchored proteins, CD14 accumulates on the cell surface in microdomains known as lipid rafts. CD14 is expressed pre dominantly on the surface of 'myeloid' cells, such as monocytes, macrophages and neutrophils, but at lower levels also on epithelial cells, endothelial cells and fibroblasts.
CD14 is also expressed in a soluble form (sCD14). sCD14 may result from secretion of the protein before coupling to the GPI anchor or from shedding or cleavage from the surface of monocytes. sCD14 is present in the circulation and other body fluids and levels of sCD14 in plasma increase during inflammation and infection. CD14 is a molecule with a broad range of functions. In addition to functioning as a pattern recognition receptor for a variety of microbial ligands, CD14 also acts as a receptor for endogenous molecules like intercellular adhesion molecule (ICAM)-3 on the surface of apoptotic cells, amyloid peptid, ceramide, and urate crystals. Ligation of CD14 by these ligands, except for apoptotic cells, mediates activation of inflammatory responses.

General readings

1. Von Wolff M. et al., Mol. Hum. Reprod., 2002; 8(12) : 1096-1102.
2. Juan TS, Hailman E, Kelley MJ, Wright SD, Lichenstein HS: Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding. J Biol Chem. 1995 Jul 21;270(29):17237-42.
3. Lodrup Carlsen KC, Granum B: Soluble CD14: role in atopic disease and recurrent infections, including otitis media. Curr Allergy Asthma Rep. 2007 Nov;7(6):436-43.
4. Asai Y, Makimura Y, Kawabata A, Ogawa T: Soluble CD14 Discriminates Slight Structural Differences between Lipid As That Lead to Distinct Host Cell Activation. J Immunol. 2007 Dec 1;179(11):7674-83.
5. Fernandez-Real JM, Broch M, Richart C, Vendrell J, Lopez-Bermejo A, Ricart W: CD14 monocyte receptor, involved in the inflammatory cascade, and insulin sensitivity. J Clin
Endocrinol Metab. 2003 Apr;88(4):1780-4.
6. Goyert SM, Ferrero E, Rettig WJ, Yenamandra AK, Obata F, Le Beau MM: The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors.
Science. 1988 Jan 29;239(4839):497-500.
7. Kuusniemi AM, Lapatto R, Holmberg C, Karikoski R, Rapola J, Jalanko H.: Kidneys with heavy proteinuria show fibrosis, inflammation, and oxidative stress, but no tubular
phenotypic change. Kidney Int. 2005 Jul;68(1):121-32.
8. Wiendl H, Mitsdoerffer M, Schneider D, Melms A, Lochmuller H, Hohlfeld R, Weller M: Muscle fibres and cultured muscle cells express the B7.1/2-related inducible co-stimulatory molecule, ICOSL: implications for the pathogenesis of inflammatory myopathies. Brain. 2003 May;126(Pt 5):1026-35.
9. Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD14 antibody

CD14 Mouse IgG2b FWKW Purified Hu F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD14 antibody

CD14 Mouse IgG2b FWKW PE Hu F
100 Tests / €300.00
  Acris Antibodies GmbH

CD14 antibody

Staining of human peripheral blood monocytes probed with Mouse anti Human CD14 - RPE Mouse IgG2a Tük4 PE Bov, Can, Gt, Hu, Por, Rb, Sh F
100 Tests / €460.00
  Acris Antibodies GmbH

CD14 antibody

Staining of human peripheral blood monocytes probed with Mouse anti Human CD14 - RPE Mouse IgG2a Tük4 PE Bov, Can, Fe, Gt, Hu, Mink, Mky, Por, Rb, Sh F
25 Tests / €210.00
  Acris Antibodies GmbH

CD14 antibody

Fig. 1. Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18). 
Lane 1: whole cell lysate HEK 293 transfected with empty vector; 
Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA; 
Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA Mouse IgG1 MEM-18 Biotin Hu, Mky F
0.1 mg / €260.00
  Acris Antibodies GmbH

CD14 (N-term) antibody

Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (N-term) (Cat.#TA302271). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD14 antibody

Fig. 1. Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18). 
Lane 1: whole cell lysate HEK 293 transfected with empty vector; 
Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA; 
Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA Mouse IgG1 MEM-18 APC Hu, Mky F
100 Tests / €290.00
  Acris Antibodies GmbH

CD14 antibody

Western Blotting analysis (non-reducing conditions) of over-expressed Human CD14 using anti-CD14 antibody (Clone MEM-18). 
Lane 1: Whole cell lysate HEK 293 transfected with empty vector. 
Lane 2: Tissue culture supernatant collected after cultivation of HEK 293 transfected with Human CD14 cDNA. 
Lane 3: Whole cell lysate of HEK 293 transfected with Human CD14 cDNA. Mouse IgG1 MEM-18 Azide Free Hu, Prim E, F, FN, IP, WB
0.1 mg / €210.00
  Acris Antibodies GmbH

CD14 antibody

Surface staining of human peripheral blood leukocytes using anti-human CD14 (clone MEM-18) FITC (SM3009F). Mouse IgG1 MEM-18 FITC Hu, Prim F
100 Tests / €260.00
  Acris Antibodies GmbH

CD14 antibody

Fig. 1. Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18). 
Lane 1: whole cell lysate HEK 293 transfected with empty vector; 
Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA; 
Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA Mouse IgG1 MEM-18 PE Hu, Prim F
100 Tests / €290.00
  Acris Antibodies GmbH

CD14 antibody

Surface staining of human peripheral blood cells with anti-human CD14 (MEM-15) Alexa Fluor® 700. Mouse IgG1 MEM-15 APC Hu, Prim F
100 Tests / €290.00
  Acris Antibodies GmbH

CD14 antibody

Surface staining of human peripheral blood cells with anti-human CD14 (MEM-15) Alexa Fluor® 700. Mouse IgG1 MEM-15 Biotin Hu, Prim F
0.1 mg / €260.00
  Acris Antibodies GmbH

CD14 antibody

Surface staining of human peripheral blood cells with anti-human CD14 (MEM-15) Alexa Fluor® 700. Mouse IgG1 MEM-15 FITC Hu, Prim F
100 Tests / €260.00
  Acris Antibodies GmbH

CD14 antibody

Surface staining of human peripheral blood cells with anti-human CD14 (MEM-15) PE. Mouse IgG1 MEM-15 Purified Hu, Prim F, IP
0.1 mg / €210.00
  Acris Antibodies GmbH

CD14 antibody

Surface staining of human peripheral blood cells with anti-human CD14 PE (SM3163R). Mouse IgG1 MEM-15 PerCP Hu, Prim F
100 Tests / €290.00
  Acris Antibodies GmbH

CD14 antibody

Surface staining of human peripheral blood cells with anti-human CD14 (MEM-15) PE. Mouse IgG1 MEM-15 PE Hu, Prim F
100 Tests / €290.00
  Acris Antibodies GmbH

CD14 antibody

Immunohistochemistry-Paraffin: CD14 Antibody (5A3B11B5) [NB100-2191] -  Immunohistochemical analysis of paraffin-embedded human lymphnode, showing membrane localization using CD14 mouse mAb with DAB staining. Mouse IgG1 5A3B11B5 Purified Hu E, F, P
0.1 ml / €380.00
  Novus Biologicals Inc.

CD14 antibody

Flow cytometry / FACS analysis: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE-Alexa Fluor® 750 Mouse IgG2a Hu E, F
0.1 mg / €340.00
  Novus Biologicals Inc.

CD14 antibody

TA303082 (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

CD14 (C-term) antibody

CD14 Antibody (C-term) (Cat.#TA302272) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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