CD140a / PDGFRA antibody

Principal name

CD140a / PDGFRA antibody

Alternative names for CD140a / PDGFRA antibody

PDGF-R-alpha, PDGF Receptor alpha, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A

SwissProt ID

P16234 (Human), P20786 (Rat), P26618 (Mouse), Q9PUF6 (Chick)

Gene ID

5156 (PDGFRA), 18595 (Pdgfra), 515610 (ACTA2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), African clawed frog, Xen (Xenopus), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Functional assay (FN)

Background of CD140a / PDGFRA antibody

Platelet-Derived Growth Factor (PDGF) in serum is the principal mitogen present for cells of mesenchymal origin. PDGF is localized in alpha-granules of platelets and released during clot formation. PDGF from human platelets has been purified and described as a cationic glycoprotein (pI 9.5 to 10.4) having a molecular weight of approximately 30 kD and composed of two covalently linked subunits, designated as chains A (16 kD) and B (14 kD). In platelets, approximately 70% of the PDGF is present as the AB dimer, with most of the remainder as BB.Purified human PDGF shows substantial size heterogeneity, ranging from 27 to 31 kD, probably due to the presence of isoforms, glycosylation processing, aging of the platelets, and partial proteolysis during purification. The A and B chains are 40% homologous in sequence and are encoded by distinctly different genes. Each chain contains 8 cysteine residues, which are involved in intra- and inter-chain disulfide bonds. Cleavage of these bonds by reduction causes irreversible loss of biological activity. PDGF elicits multifunctional actions with a variety of cells.Two distinct human PDGF receptors have been identified, PDGF alpha and PDGF beta, which are structurally related, consist of an extracellular region, a single transmembrane region, and an intracellular region. The three different isoforms of PDGF (PDGF-AA, PDGF-AB, and PDGF-BB) bind with different affinities to two both receptors. Ligand binding induces receptor dimerization. The A-subunit of PDGF binds to alpha-receptors, whereas the B-subunit binds to both alpha- and beta- receptors. Binding of PDGF to its receptor activates the tyrosine kinase domain and leads to enhanced phosphorylation of intracellular substrates as well as autophosphorylation of the receptor itself. Autophosphorylation is induced by allowing binding and activation of the cytoplasmic SH2-domain, which contains signal transduction molecules. Thereby, a number of different signaling pathways are initiated leading to cell growth, actin reorganization, migration and differentiation.

General readings

General / background references:Rolny C et al. Heparin amplifies platelet-derived growth factor (PDGF)- BB-induced PDGF alpha -receptor but not PDGF beta -receptor tyrosine phosphorylation in heparan sulfate-deficient cells. Effects on signal transduction and biological responses. J Biol Chem 277:19315-21 (2002). PubMed PMID: 11912193
Carloni V et al. Cell adhesion regulates platelet-derived growth factor-induced MAP kinase and PI-3 kinase activation in stellate cells. Hepatology 36:582-91 (2002). PubMed PMID: 12198650
Heldin CH et al. Signal transduction via platelet-derived growth factor receptors. Biochim Biophys Acta 1378:F79-113 (1998). PubMed PMID: 9739761
Yu JC et al. Biological function of PDGF-induced PI-3 kinase activity: its role in alpha PDGF receptor-mediated mitogenic signaling. J Cell Biol 127:479-87 (1994). PubMed PMID: 7929590

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD140a / PDGFRA antibody

Western blot analysis of extracts from HepG2 cells, using PDGFR a antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140a / PDGFRA antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PDGFRA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804956) Mouse IgG2a OTI2E9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140a / PDGFRA antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PDGFRA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA804990)(1:150) Mouse IgG1 OTI6E2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140a / PDGFRA antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PDGFRA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807645)(1:150) Mouse IgG1 OTI1C10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140a / PDGFRA antibody

AP15723PU PDGFR alpha antibody staining of Human Ovarian Carcinoma. Rabbit Purified Hu, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD140a / PDGFRA antibody

AP15723PU PDGFR alpha antibody staining of Human Ovarian Carcinoma. Rabbit Purified Hu, Rt P
1 ml / €440.00
  Acris Antibodies GmbH

CD140a / PDGFRA antibody

AP15723PU PDGFR alpha antibody staining of Human Ovarian Carcinoma. Rabbit Purified Hu, Rt P
0.1 ml / €180.00
  Acris Antibodies GmbH

CD140a / PDGFRA antibody

Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE .  Total viable cells were used for analysis. Rat APA5 Purified Ms F, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD140a / PDGFRA antibody

Western blot analysis of PDGFRa expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140a / PDGFRA antibody

Western blot analysis on 293 cell lysate using PDGFRalpha Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD140a / PDGFRA antibody

Western blot analysis of PDGFR a expression in HepG2 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD140a / PDGFRA antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFRA (RC211740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDGFRA. Mouse IgG1 OTI2B12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140a / PDGFRA antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFRA (RC211740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDGFRA.(1:2000) Mouse IgG2a OTI1H10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140a / PDGFRA antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFRA (RC211740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDGFRA. Mouse IgG1 OTI4E7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140a / PDGFRA antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFRA (RC211740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDGFRA. Mouse IgG1 OTI1A11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140a / PDGFRA antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFRA (RC211740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDGFRA. Mouse IgG2b OTI3F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140a / PDGFRA antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFRA (RC211740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDGFRA.(1:2000) Mouse IgG2b OTI4G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140a / PDGFRA antibody

CD140a / PDGFRA Mouse IgG1 16A1 APC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD140a / PDGFRA antibody

CD140a / PDGFRA Mouse IgG1 16A1 Biotin Hu F
0.1 mg / €260.00
  Acris Antibodies GmbH

CD140a / PDGFRA antibody

CD140a / PDGFRA Mouse IgG1 16A1 PE Hu
100 Tests / €290.00
  Acris Antibodies GmbH

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