CD140b / PDGFRB antibody

Principal name

CD140b / PDGFRB antibody

Alternative names for CD140b / PDGFRB antibody

PDGF-R-beta, PDGF Receptor beta, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B

SwissProt ID

P05622 (Mouse), P09619 (Human), Q05030 (Rat), Q6QNF3 (Canfa)

Gene ID

18596 (Pdgfrb), 5159 (PDGFRB)

Available reactivities

Ms (Mouse), Hu (Human), Mky (Monkey), Rt (Rat), Bab (Baboon), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit), Eq (Equine)

Available hosts

Goat, Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Neutralisation (NEUT)

Background of CD140b / PDGFRB antibody

Platelet derived growth factor (PDGF), the major mitogen in serum for cultured connective tissue cells, exerts it actions via specific receptors on the cell surface. Two distinct human PDGF receptor transmembrane binding proteins have been identified, a 170 kDa, 1066 amino acid residue alpha-receptor (PDGF Ralpha) and a 190 kDa, 1074 amino acid residue beta-receptor (PDGF Rbeta). The two receptor proteins are structurally related and consist of an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic SH2-domain containing signal transduction molecules. Thereby, a number of different signaling pathways are initiated leading to cell growth, actin reorganization, migration and differentiation. Recent observations suggest that extensive cross-talk occurs between different signaling pathways, and that stimulatory signals are modulated by inhibitory signals arising in parallel. Between the two PDGF receptors, there is 44% overall sequence identity. Within the extracellular domain, 30% of the amino acid residues are identical. The different isoforms of PDGF (PDGF-AA, PDGF-AB and PDGF-BB) bind with different affinities to two distinct receptors. Ligand-binding induces receptor dimerization; the A-subunit of PDGF binds to alpha-receptors, whereas the B-subunit binds to both alpha- and beta-receptors. Binding of PDGF to its receptor activates the tyrosine kinase domain and leads to enhanced phosphorylation of intracellular substrates as well as to autophosphorylation of the receptor itself. In addition, several other cellular responses are induced. Studies have indicated that PDGF beta-receptors are not present on most cells of normal tissues, but are upregulated, in conjunction with inflammation, excess cell proliferation, malignancytic, and fibrotic conditions.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD140b / PDGFRB antibody

Surface staining of CD140b / PDGF-RB in human CD140b / PDGF-RB stable transfectants with anti-CD140b (18A2) APC. Mouse IgG1 18A2 Purified Hu C, F, ICC/IF
0.1 mg / €230.00
  Acris Antibodies GmbH

CD140b / PDGFRB antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDGFRB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506230) Mouse IgG1 OTI1E8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140b / PDGFRB antibody

Anti-PDGFRB mouse monoclonal antibody (TA506233) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDGFRB(RC206377). Mouse IgG2a OTI1D2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140b / PDGFRB antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PDGFRB and PLCG1. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CD140b / PDGFRB antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-PDGFRB mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 4C12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD140b / PDGFRB antibody

Immunohistochemistry: PDGF Receptor beta Antibody [NBP1-32601] - analysis of paraffin-embedded U87 xenograft, using PDGF Receptor beta(CD140b) antibody at 1:500 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

CD140b / PDGFRB antibody

Human ovarian carcinoma stained with anti-PDGFR-beta antibody Rabbit Purified Hu, Ms P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD140b / PDGFRB antibody

Human ovarian carcinoma stained with anti-PDGFR-beta antibody Rabbit Purified Hu, Ms P
1 ml / €440.00
  Acris Antibodies GmbH

CD140b / PDGFRB antibody

Human ovarian carcinoma stained with anti-PDGFR-beta antibody Rabbit Purified Hu, Ms P
0.1 ml / €180.00
  Acris Antibodies GmbH

CD140b / PDGFRB antibody

Western blot analysis of extracts from LOVO cells, treated with H2O2 (100uM, 30mins), using PDGFR ß (Ab-1021) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

Western blot analysis of extracts from HepG2 cells, using PDGFRb (Ab-771) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE .  Total viable cells were used for analysis. Rat APB5 Purified Ms F, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

Predicted band size: 124 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 90 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

Predicted band size: 124 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB (N-term) antibody

Western blot analysis of anti-PDGFRB Antibody (N-term) (Cat. #TA324967) in cell line lysates (35ug/lane). PDGFRB(arrow) was detected using the purified Pab). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

Western blot analysis of PDGFR beta expression in TNF-a treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

Western blot analysis of PDGFR beta expression in H2O2 treated LOVO whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

Western blot analysis of PDGFRb expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

Western blot analysis of extracts of various cell lines, using PDGFRB antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB (Extracell. Dom.) antibody

Anti-PDGFRB antibody IHC of human kidney, renal tubular epithelium in cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

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