CD140b / PDGFRB antibody

Principal name

CD140b / PDGFRB antibody

Alternative names for CD140b / PDGFRB antibody

PDGF-R-beta, PDGF Receptor beta, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B

SwissProt ID

P05622 (Mouse), P09619 (Human), Q05030 (Rat), Q6QNF3 (Canfa)

Gene ID

5159 (PDGFRB), 18596 (Pdgfrb), 24629

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of CD140b / PDGFRB antibody

Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kises, PDGF receptor a (PDGFRa) and PDGF receptor ß (PDGFRß).PDGFRa and PDGFRß share 75% to 85% sequence homology between their two intracellular kise domains, while the kise insert and carboxy-termil tail regions display a lower level (27% to 28%) of homology. PDGFRa homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRß homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor a/ß binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer. PDGFRa and PDGFRß can each form heterodimers with EGFR, which is also activated by PDGF. Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding. Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing sigl transduction molecules, such as GRB2, Src, GAP, PI3 kise, PLC?, and NCK. A number of different sigling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation. Tyr751 in the kise-insert region of PDGFRß is the docking site for PI3 kise. Phosphorylated pentapeptides derived from Tyr751 of PDGFRß (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-termil SH2 domain of the p85 subunit of PI3 kise with PDGFRß. Tyr740 is also required for PDGFRß-mediated PI3 kise activation.

General readings

1. Andrae J, Gallini R, Betsholtz C. Role of platelet-derived growth factors in physiology and medicine. Genes Dev. 2008 May 15;22(10):1276-312. doi: 10.1101/gad.1653708. PubMed PMID: 18483217.
2. French WJ, Creemers EE, Tallquist MD. Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol. 2008 Sep;28(18):5646-57. doi: 10.1128/MCB.00441-08. Epub 2008 Jul 7. PubMed PMID: 18606782.
3. Schmahl J, Rizzolo K, Soriano P. The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev. 2008 Dec 1;22(23):3255-67. doi: 10.1101/gad.1723908. PubMed PMID: 19056881.
4. Faraone D, Aguzzi MS, Toietta G, Facchiano AM, Facchiano F, Magenta A, et al. Platelet-derived growth factor-receptor alpha strongly inhibits melanoma growth in vitro and in vivo. Neoplasia. 2009 Aug;11(8):732-42. PubMed PMID: 19649203.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD140b / PDGFRB antibody

Surface staining of CD140b / PDGF-RB in human CD140b / PDGF-RB stable transfectants with anti-CD140b (18A2) APC. Mouse IgG1 18A2 Purified Hu C, F, ICC/IF
0.1 mg / €230.00
  Acris Antibodies GmbH

CD140b / PDGFRB antibody

  PDGFRB mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDGFRB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506230) Mouse IgG1 OTI1E8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140b / PDGFRB antibody

  PDGFRB mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)  
Anti-PDGFRB mouse monoclonal antibody (TA506233) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDGFRB(RC206377). Mouse IgG2a OTI1D2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD140b / PDGFRB antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PDGFRB and PLCG1. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CD140b / PDGFRB antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-PDGFRB mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 4C12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD140b / PDGFRB antibody

  Rabbit polyclonal PDGFR beta (Ab-1021) antibody  
Western blot analysis of extracts from LOVO cells, treated with H2O2 (100uM, 30mins), using PDGFR ß (Ab-1021) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Rabbit polyclonal PDGFRb (Tyr771) antibody(Phospho-specific)  
Western blot analysis of extracts from HepG2 cells, using PDGFRb (Phospho-Tyr771) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Rabbit polyclonal PDGFRb (Ab-771) antibody  
Western blot analysis of extracts from HepG2 cells, using PDGFRb (Ab-771) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Rat Anti-Mouse CD140b (PDGF Receptor b) Purified (100 ug)  
Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE .  Total viable cells were used for analysis. Rat APB5 Purified Ms F, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Anti-PDGFRB Rabbit Polyclonal Antibody  
Predicted band size: 124 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB (N-term) antibody

  Rabbit polyclonal PDGFRB Antibody (N-term)  
Western blot analysis of anti-PDGFRB Antibody (N-term) (Cat. #TA324967) in cell line lysates (35ug/lane). PDGFRB(arrow) was detected using the purified Pab). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Rabbit Polyclonal PDGFR beta Antibody  
Western blot analysis of PDGFR beta expression in TNF-a treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Rabbit Polyclonal PDGFR beta Antibody  
Western blot analysis of PDGFR beta expression in H2O2 treated LOVO whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Rabbit Polyclonal PDGFRb Antibody  
Western blot analysis of PDGFRb expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Rabbit anti-PDGFRB Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PDGFRB antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Rabbit Polyclonal Phospho-PDGFR beta (Tyr1021) Antibody (Phospho-specific)  
CD140b / PDGFRB Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Rabbit Polyclonal Phospho-PDGFRb (Tyr771) Antibody (Phospho-specific)  
Western blot analysis of PDGFRb phosphorylation expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD140b / PDGFRB (Extracell. Dom.) antibody

  Rabbit Polyclonal Anti-PDGFRB Antibody (Extracellular Domain)  
Anti-PDGFRB antibody IHC of human kidney, renal tubular epithelium in cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

CD140b / PDGFRB (Extracell. Dom.) antibody

  Rabbit Polyclonal Anti-PDGFRB Antibody (Extracellular Domain)  
Anti-PDGFRB antibody IHC of human kidney, glomerulus and renal tubular epithelium in cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

CD140b / PDGFRB antibody

  Rabbit Polyclonal anti-PDGFR beta Antibody  
Human Ovarian Carcinoma stained with anti-PDGFR beta antibody. Rabbit Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

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