CD142 / Tissue factor antibody

Principal name

CD142 / Tissue factor antibody

Alternative names for CD142 / Tissue factor antibody

TF, F3, Coagulation factor III, Thromboplastin

SwissProt ID

P13726 (Human), P20352 (Mouse), P30931 (Bovin), P42533 (Rat)

Gene ID

2152 (F3)

Available reactivities

Hu (Human), Prim (Primate), Ms (Mouse), Mky (Monkey), Rt (Rat)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of CD142 / Tissue factor antibody

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.

General readings

1. Morrissey, J. H. et al. (1988) Monoclonal antibody analysis of purified and cell-associated tissue factor. Thromb. Res. 52: 247-261.
2. Contrino, J. et al. (1994) In situ characterization of antigenic and functional tissue factor expression in human tumors utilizing monoclonal antibodies and recombinant factor VIIa as probes. Am. J. Path. 145: 1315-1322.
3. Drake, T. A. et al. (1989) Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis. Am. J. Pathol. 134: 1087-1097.
4. Stearns-Kurosawa, D. J. et al. (2006) Sepsis and pathophysiology of anthrax in a nonhuman primate model. Am. J. Pathol. 169: 433-444.
5. Mueller, B. M. et al. (1992) Expression of tissue factor by melanoma cells promotes efficient hematogenous metastasis. Proc. Natl. Acad. Sci. U. S. A. 89: 11832-11836.
6. Agmon-Levin, N. et al. (2011) Vitamin D: an instrumental factor in the anti-phospholipid syndrome by inhibition of tissue factor expression. Ann Rheum Dis. 70: 145-50.
7. Ruf, W. et al. (1991) Rehemtulla A, Edgington TS. Antibody mapping of tissue factor implicates two different exon-encoded regions in function. Biochem. J. 278: 729-33.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD142 / Tissue factor antibody

CD142 / Tissue factor Mouse IgG1 CLB/TF-5 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD142 / Tissue factor antibody

CD142 / Tissue factor Mouse IgG1 CLB/TF-5 FITC Hu F
25 µg / €190.00
  Acris Antibodies GmbH

CD142 / Tissue factor antibody

F3 MaxPab rabbit polyclonal antibody. Western Blot analysis of F3 expression in mouse liver. Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

CD142 / Tissue factor antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between F7 and F3. HeLa cells were stained with anti-F7 rabbit purified polyclonal 1:1200 and anti-F3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 4G4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD142 / Tissue factor antibody

Predicted band size: 33 kDa . Positive control: Human fetal lung tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD142 / Tissue factor antibody

Predicted cell location: Membrane,Cytoplasm. Positive control: Human ovarian and thyroid cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using F3 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD142 / Tissue factor antibody

CD142 / Tissue factor Mouse IgG1 CLB/TF-1 Purified Hu E, F
0.2 mg / €430.00
  Acris Antibodies GmbH

CD142 / Tissue factor antibody

CD142 / Tissue factor Mouse IgG1 CLB/TF-1 Purified Hu E, F
25 µg / €180.00
  Acris Antibodies GmbH

CD142 / Tissue factor antibody

CD142 / Tissue factor Mouse IgG1 TF9-10H10 Purified Hu, Prim C, E, F, ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD142 / Tissue factor antibody

F3 MaxPab rabbit polyclonal antibody. Western Blot analysis of F3 expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CD142 / Tissue factor antibody

CD142 / Tissue factor Mouse IgG2a 4G4 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

CD142 / Tissue factor pSer290 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Coagulation Factor III (Phospho-Ser290) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
50 µg / €205.00
  Assay Biotech

CD142 / Tissue factor pSer290 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Coagulation Factor III (Phospho-Ser290) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €310.00
  Assay Biotech

CD142 / Tissue factor antibody

CD142 / Tissue factor Mouse IgG1 CLB/TF-1 Aff - Purified Hu E, F
0.1 mg / €360.00
  Novus Biologicals Inc.

CD142 / Tissue factor antibody

CD142 / Tissue factor Mouse IgG1 TF9-10H10 Purified Hu, Mky C, E, F, ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD142 / Tissue factor

CD142 / Tissue factor Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CD142 / Tissue factor

CD142 / Tissue factor Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €399.00
  OriGene Technologies, Inc.

CD142 / Tissue factor (33-251, His-tag)

CD142 / Tissue factor Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

CD142 / Tissue factor (33-251, His-tag)

CD142 / Tissue factor Human Purified > 90 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

CD142 / Tissue factor (29-251, His-tag)

CD142 / Tissue factor Mouse Purified > 95 % by SDS - PAGE
0.25 mg / €940.00
  Acris Antibodies GmbH

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