CD144 / Cadherin-5 antibody

Principal name

CD144 / Cadherin-5 antibody

Alternative names for CD144 / Cadherin-5 antibody

VE-Cadherin, Vascular endothelial cadherin, CDH5, 7B4 antigen

SwissProt ID

O02840 (Pig), P33151 (Human), P55284 (Mouse), Q6URK6 (Bovin)

Gene ID

1003 (CDH5), 414735, 999 (CDH1), 414737, 12562 (Cdh5)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Por (Porcine), Mky (Monkey)

Available hosts

Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Enzyme Immunoassay (E), Functional assay (FN), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoelectron Microscopy (EM)

Background of CD144 / Cadherin-5 antibody

The monoclonal antibody BV9 binds to the extracellular domain (EC3-EC4) of human VE-cadherin (vascular endothelial cadherin). Endothelial cells control the passage of plasma constituents and circulating cells from blood to the underlying tissues. VE-cadherin is of vital importance for the maintenance and control of endothelial cell contacts. Mechanisms that regulate VE-cadherin- mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. VE-cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Therefore, VE-cadherin is also essential during embryonic angiogenesis. The specialized function of VE-cadherin is lost or impaired in several pathological conditions - including inflammation, sepsis, ischemia and diabetes - which leads to severe, and sometimes fatal, organ dysfunction. Furthermore, abnormal increase in vascular permeability is often observed in pathological conditions, such as tumor-induced angiogenesis, macular degeneration, allergy, and brain stroke. Endothelial permeability is regulated in part by the dynamic opening and closure of cell-cell adherent junctions. In vascular endothelium, adherent junctions are mainly composed of VE-cadherin, an adhesive receptor that is able to self-associate at endothelial cell -cell contacts. VE-cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. VE-cadherin belongs to the cadherin super-family of cell - cell adhesion molecules, which are encoded by more than 200 genes in the human genome. Classical cadherins are Ca2+-dependent, homophilic, cell to cell adhesion molecules expressed in nearly all cells within solid tissues. Cadherins form a core adhesion complex that consists of a cadherin dimer, binding through its extracellular region to another dimer of cadherins expressed in adjacent cells, while its intracellular region is anchored to the plasma membrane and linked to the cytoskeleton. The VE-cadherin extracellular domain consists of five cadherin-type repeats, called EC (extracellular cadherin) domains that are bound together by calcium ions in a rod-like structure.

General readings

General / background references:Navarro P et al. Catenin-dependent and -independent functions of vascular endothelial cadherin. J Biol Chem 270:30965-72 (1995). PubMed PMID: 8537353
Lampugnani MG et al. The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, beta-catenin, and alpha-catenin with vascular endothelial cadherin (VE-cadherin). J Cell Biol 129:203-17 (1995). PubMed PMID: 7698986
Breviario F et al. Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin. Arterioscler Thromb Vasc Biol 15:1229-39 (1995). PubMed PMID: 7627717
Martin-Padura I et al. Expression of VE (vascular endothelial)-cadherin and other endothelial-specific markers in haemangiomas. J Pathol 175:51-7 (1995). PubMed PMID: 7891227

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD144 / Cadherin-5 antibody

CDH5 polyclonal antibody (A01), Lot # 051003JCO1. Western Blot analysis of CDH5 expression in NIH/3T3. Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CD144 / Cadherin-5 antibody

Western blot analysis of extracts from Jurkat cells, using VE-Cadherin (Ab-731) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were resolved by SDS -PAGE and immunoblotted with 2 ug/mL of Anti-Human CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP. Mouse 16B1 Purified Hu F, ICC/IF, WB
25 µg / €120.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

Staining of bEnd.3 cell line with 0.5 ug of Rat IgG1 K Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD144 (VE-Cadherin) Purified (filled histogram) followed by F(ab')2 Anti-Rat IgG PE .  Total viable cells were used for analysis. Rat eBioBV13 (BV13) Purified Ms F
50 µg / €120.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

Non-reduced (left) and reduced (right) bEnd.3 cell line lysates were loaded at 1x10e5 cells/lane, probed with 2 ug/mL of Anti-Mouse CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP. Rat eBioBV14 (BV14) Purified Ms IP, P, WB
50 µg / €120.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid and ovarian cancer tissue tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue tissue using CDH5) at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

Predicted cell location: Cytoplasm. Positive control: Human tonsil and liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using CHD5 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

Western blot analysis of VE-Cadherin expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

ELISA: VE Cadherin Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

CD144 / Cadherin-5 Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

ELISA: VE Cadherin Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

ELISA: VE Cadherin Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD144 / Cadherin-5 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDH5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804746) Mouse IgG1 OTI1F4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD144 / Cadherin-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH5 (RC223907, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH5. Mouse IgG1 OTI1E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD144 / Cadherin-5 (Extracell. Dom) antibody

Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI. Mouse IgG2a BV9 FITC Hu C, E, FN, ICC/IF, IP, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

CD144 / Cadherin-5 (Extracell. Dom) antibody

Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI. Mouse IgG2a BV9 Purified Hu C, E, FN, ICC/IF, IP, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

CD144 / Cadherin-5 antibody

CD144 / Cadherin-5 Mouse IgG1 55-7H1 Purified Hu ICC/IF
0.1 mg / €210.00
  Acris Antibodies GmbH

CD144 / Cadherin-5 (N-term) antibody

Western blot analysis of anti-CDH5 (N-term) Pab (Cat.#AP12345PU-N) in 293 cell line lysates (35ug/lane). CDH5 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD144 / Cadherin-5 pTyr731 antibody

Western blot analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) antibody AP55902PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

CD144 / Cadherin-5 pTyr731 antibody

Western blot analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) antibody AP55902PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

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