CD146 antibody

Principal name

CD146 antibody

Alternative names for CD146 antibody

MCAM, MUC18, CD146, Melanoma-associated antigen MUC18, A32, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Cell surface glycoprotein P1H12

SwissProt ID

P43121 (Human), Q8R2Y2 (Mouse), Q9EPF2 (Rat)

Gene ID

4162 (MCAM)

Available reactivities

Hu (Human), Por (Porcine), Can (Canine), Ms (Mouse), Rb (Rabbit), Chk (Chicken), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of CD146 antibody

CD146 (also known as A32, MCAM, Mel-CAM, MUC18, and S-Endo-1) belongs to the immunoglobulin supergene family with five immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell-cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N-linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate-3-glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Overexpression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

General readings

1. Vainio, O., D. Dunon, F. Aissi, J.-P. Dangy, K.M. McNagny, and B.A. Imhof. 1996. HEMCAM, an adhesion molecules
expressed by c-kit+ hemopoietic progenitors. J. Cell. Biol. 135:1655-1668.
2. Vainio, O. 1998. Personal communication.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD146 antibody

CD146 Mouse IgG1 OJ79c FITC Hu, Por C, E, F
0.1 mg / €510.00
  Acris Antibodies GmbH

CD146 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min) Mouse IgG1 UMAB154 Purified Hu P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD146 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min) Mouse IgG2b UMAB155 Purified Hu P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD146 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min) Mouse IgG1 UMAB154 Purified Hu P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD146 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MCAM mouse monoclonal antibody. (UM800052; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min) Mouse IgG2b UMAB155 Purified Hu P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD146 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MCAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803398) Mouse IgG2b OTI2G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD146 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MCAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803468) Mouse IgG1 OTI2B2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD146 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MCAM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803548) Mouse IgG1 OTI5C4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD146 antibody

Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. Mouse IgG1 P1H12 Aff - Purified Hu C, F, IP, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

CD146 antibody

Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.125 ug of Mouse IgG1 K Isotype Control Purified (blue histogram) or 0.125 ug of Anti-CD146 Purified (purple histogram) followed by F(ab')2 Anti-Mouse IgG PE .  Total viable cells were used for analysis. Mouse P1H12 Purified Can, Hu, Ms, Rb F, IP, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD146 antibody

Predicted band size: 72 kDa . Positive control: Human leiomyosarcoma tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/850 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD146 antibody

Predicted band size: 72 kDa . Positive control: Human fetal intestines tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD146 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCAM (RC208937, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCAM. Mouse IgG1 OTI3D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD146 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCAM (RC208937, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCAM. Mouse IgG1 OTI1F10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD146 antibody

CD146 Mouse IgG2b c264 Biotin Chk F
0.5 mg / €410.00
  Acris Antibodies GmbH

CD146 antibody

CD146 Mouse IgG2b c264 FITC Chk F
0.5 mg / €450.00
  Acris Antibodies GmbH

CD146 antibody

Immunofluorescent Staining: Thymocytes from a young chicken were isolated and incubated with purified Mouse anti-Chicken MCAM. The
cells were then stained with Goat anti-Mouse IgG2b-FITC and analyzed on a FACScan(TM) flow cytometer (BDB, San Jose, CA). Amount Used: 1 µg/10e6 cells. Mouse IgG2b c264 Purified Chk C, F, IP
0.5 mg / €340.00
  Acris Antibodies GmbH

CD146 antibody

CD146 Mouse IgG2b c264 PE Chk F
0.1 mg / €320.00
  Acris Antibodies GmbH

CD146 antibody

Western blot analysis of MCAM Antibody (Center) (AP18112PU-N) in A2058 cell line lysates (35ug/lane). MCAM (arrow) was detected using the purified Pab.(2ug/ml) Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD146 antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with MCAM antibody AP22800PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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