CD146 antibody

Principal name

CD146 antibody

Alternative names for CD146 antibody

MCAM, MUC18, CD146, Melanoma-associated antigen MUC18, A32, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Cell surface glycoprotein P1H12

SwissProt ID

P43121 (Human), Q8R2Y2 (Mouse), Q9EPF2 (Rat)

Gene ID

4162 (MCAM), 84004 (Mcam), 78967

Available reactivities

Hu (Human), Por (Porcine), Chk (Chicken), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD146 antibody

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety.
Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Overexpression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

General readings

1. Vainio, O., D. Dunon, F. Aissi, J.-P. Dangy, K.M. McNagny, and B.A. Imhof. 1996. HEMCAM, an adhesion molecules
expressed by c-kit+ hemopoietic progenitors. J. Cell. Biol. 135:1655-1668.
2. Vainio, O. 1998. Personal communication.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD146 antibody

Western blot analysis of MCAM Antibody (Center) (AP18112PU-N) in A2058 cell line lysates (35ug/lane). MCAM (arrow) was detected using the purified Pab.(2ug/ml) Rabbit Hu WB
0.4 ml / €370.00
  OriGene Technologies GmbH

CD146 antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with MCAM antibody AP22800PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu P, WB
50 µg / €380.00
  OriGene Technologies GmbH

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