CD152 / CTLA4 antibody

Principal name

CD152 / CTLA4 antibody

Alternative names for CD152 / CTLA4 antibody

CTLA-4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4

SwissProt ID

P09793 (Mouse), P16410 (Human), Q62859 (Rat), Q9MYX7 (Pig), Q9XSI1 (Canfa)

Gene ID

1493 (CTLA4), 63835 (Ctla4), 12477

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Goat, Hamster, Rabbit, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Functional assay (FN), Flow Cytometry (F), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Neutralisation (NEUT), Immunoprecipitation (IP), Dot blot (Dot), Paraffin Sections (P)

Background of CD152 / CTLA4 antibody

The vertebrate immune system has an important self check mechanism that prevents it from destroying the host own immune system and regulatory T cells are part of this regulatory suppressive control. The cytotoxic T lymphocyte antigen 4 (CTLA4) is a protein that is expressed on regulatory T lymphocytes and is involved in restraining the immune response when needed and in tachyphylaxis and tolerance. The Fox3p-CD4+ (CD25+CD4+) regulatory T cells (Tregs) express the transcription factor Foxp3 suppress aberrant immune responses as seen in autoimmune diseases and allergy (1). The two important features of Foxp3+ tregs are, first they constitutively express CTLA4 and Fox3p control the expression of CTLA4 in Tregs (1). CTLA4 is a potent negative regulator of T cell responses as illustrated in CTLA4 knockout mice which die prematurely from multiorgan inflammation (2). Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
The polymorphism in the CTLA4 gene contributes to the genetic susceptibility to autoimmune diseases such as diabetes type I (3). Various other auto immune conditions such as inflammatory bowel disease tumor immunity can be suppressed by blocking CTLA4 by CTLA4 antibody (4). By deleting the CTLA4 gene in Tregs the role of CTLA4 in maintenance of self tolerance and immune homeostasis can be studied. The Treg specific CTLA4 deficiency impairs in vivo and in vitro suppressive functions of Treg by down regulating CD80 and CD86 expression in dentritic cells (4). Thus the naturally occurring Tregs may require CTLA4 to suppress immune responses by affecting the potency of antigen-presenting cells to activate other T cells (4). The CTLA4 expressed in Fox3p positive tregs is critically required for their in vivo and in vitro suppression which is mediated in at least in part by CTLA4 dependent down regulation of CD80 and CD86 in antigen presenting cells. The CTLA4 is a key molecular target for controlling Treg-suppressive function in both physiological and pathological immune responses including autoimmunity, allergy and tumor immunity. The CTLA4 is a 223 amino acid protein with an apparent MW of 28kD. This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. CTLA4 also expressed as a larger transcript variant isoform also known as CTLA4-TM. The tyrosine at position 201 is also a substrate for phosphorylation.

General readings

1. Krummel, M.F., and J.P. Allison. 1995. J. Exp. Med. 182:59-465.
2. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1994. Immunol. Today 15:321-331.
3. Perkins, D., Z. Wang, C. Donovan, H. He, et al. 1996. J. Immunol. 156:4154-4159.
4. Alegre, M.-L., P.J. Noel, B.J. Eisfelder, E. Chuang, M.R. Clark, S.L. Reiner, and C.B. Thompson. 1996. J. Immunol. 157:4762-4770.
5. Waterhouse, P., J.M. Penninger, E. Timms, A. Wakeham, A. Shahinian, K.P. Lee, C.B. Thompson,, H. Griesser, and T.W. Mak. 1995.
Science 270:985-988.
6. Marengere, L.E.M., P. Waterhouse, G.S. Duncan, H.-W. Mittrucker, G.-S. Fneg, and T.W. Mak. 1996. Science 272:1170-1173.

  1. Quick-Filters:
  2. Highly Validated (1)

Items 1 to 20 of 75 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD152 / CTLA4 (N-term) antibody

Formalin fixed and paraffin embedded human prostate carcinoma reacted with CD152 / CTLA4 Antibody (N-term) Cat.-No AP52146PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

Western Blot: CTLA4 Antibody (37B3D8.2F2) [TA337194] - WB analysis of CTLA-4 protein on (A) mouse Kidney lysate (B) human Kidney lysate and on © partial recombinant protein using CTLA-4 monoclonal antibody at a concentration of 2 ug/ml. Mouse 37B3D8.2F2 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD152 / CTLA4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTLA4 (RC213631, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTLA4.(1:2000) Mouse IgG1 OTI8D12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD152 / CTLA4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTLA4 (RC213631, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTLA4.(1:2000) Mouse IgG1 OTI1E2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD152 / CTLA4 antibody

CD152 / CTLA4 Mouse IgG1 AS32 Purified Hu FN
0.1 mg / €400.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

CD152 / CTLA4 Hamster IgG 1B8 Biotin Ms F
0.5 mg / €330.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

Immunofluorescent Staining: CHO cells transfected with CD152/CTLA-4 were stained with either Hamster IgG-FITC or Hamster anti-Mouse CD152-FITC, following which large cells were gated and analyzed by flow cytometry. Amount Used: < / = 3 µg/10e6 cells. Hamster IgG 1B8 FITC Ms F
0.5 mg / €400.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

CD152 / CTLA4 Hamster IgG 1B8 Low Endotoxin Ms F, FN, IP
0.5 mg / €350.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

CD152 / CTLA4 Hamster IgG 1B8 Purified Ms F, FN, IP
0.5 mg / €290.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

CD152 / CTLA4 Hamster IgG 1B8 PE Ms F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

CD152 / CTLA4 Hamster IgG 9H10 Low Endotoxin Ms F, FN, IP
0.5 mg / €350.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

CD152 / CTLA4 Hamster IgG 9H10 Purified Ms F, FN, IP
0.5 mg / €290.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

CD152 / CTLA4 Mouse IgG3 MIH8 Azide Free Hu F
0.1 mg / €300.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

CD152 / CTLA4 Mouse IgG3 MIH8 Biotin Hu F
1 ml / €380.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

Cell Source: Human IL2 Activated T-Blasts 
Percentage of cells stained above control: 72.0%
Cells were fixed and permeabilized prior to AM31866FC-N staining Mouse IgG2a A3.4H2.H12 FITC Hu F
0.1 mg / €330.00
  Acris Antibodies GmbH

CD152 / CTLA4 antibody

CD152 / CTLA4 Mouse IgG2a A3.4H2.H12 Purified Hu F
0.25 mg / €400.00
  Acris Antibodies GmbH

CD152 / CTLA4 (36-161) antibody

ICC/IF analysis of CTLA4 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human CTLA4 antibody (1:100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). Mouse IgG1 AT4F2 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

CD152 / CTLA4 (36-161) antibody

ICC/IF analysis of CTLA4 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human CTLA4 antibody (1:100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). Mouse IgG1 AT4F2 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

CD152 / CTLA4 Control Peptide

CD152 / CTLA4
0.25 mg / €250.00
  Acris Antibodies GmbH

CD152 / CTLA4 (158-162) antibody

CD152 / CTLA4 Rabbit Aff - Purified Hu, Ms, Rt Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Items 1 to 20 of 75 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  • LinkedIn