CD154 / CD40L antibody

Principal name

CD154 / CD40L antibody

Alternative names for CD154 / CD40L antibody

CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, T-cell antigen Gp39

SwissProt ID

O97605 (Felca), O97626 (Canfa), P27548 (Mouse), P29965 (Human), P51749 (Bovin), Q95MQ5 (Pig), Q9Z2V2 (Rat)

Gene ID

959 (CD40LG), 21947 (Cd40lg), 2749590 (Q73CV5), 970 (CD70)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), Rhesus Mky (Rhesus Monkey), Rb (Rabbit), Sh (Sheep), Prim (Primate)

Available hosts

Rabbit, Hamster, Mouse, Rat, Goat

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Flow Cytometry (F), Functional assay (FN), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Neutralisation (NEUT), Luminex (LMNX)

Background of CD154 / CD40L antibody

CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.
The protein encoded by this gene is a member of the TNF receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

General readings

1. Noelle, R.J., M. Roy, D.M. Shepherd, I Stamenkovic, J.A. Ledbetter, and A. Aruffo. 1992. Proc. Natl. Acad. Sci. U.S.A. 89:6550.
2. Aruffo, A., M. Farrington, D. Hollenbaugh, X. Li, A. Milatovich, S. Nonoyama, J. Bajorath, L.S. Grosmaire, R. Stenkamp, and M. Neubauer.
1993. Cell 72:291.
3. Hollenbaugh, D., L.S. Grosmaire, C.D. Kullas, N.J. Chalupny, S. Braesch-Andersen, R.J. Noelle, I. Stamenkovic, J.A. Ledbetter, and A. Aruffo.
1992. EMBO J. 11:4313.
4. Foy, TM., DM Shepherd, F.H. Durie, A. Aruffo, J.A. Ledbeter, and R.J. Noelle. 1993. J. Exp. Med. 178:1567.
5. Foy, T.M., D.M. page, T.J. Waldschmidt, A. Schoneveld, J.D. Laman, S.R. Masters, L. Tygrett, J.A. Ledbetter, A. Aruffo, and E. Claassen.
1995. J. Exp. Med. 182:1377.
6. Roy, M., T.J. Waldschmidt, A. Aruffo, J.A. Ledbetter, and R.J. Noelle. 1993. J. Immunol. 151:2497.
7. Armitage, R.J., W.C. Fanslow, L. Stockbine, T.A. Sato, K.N. Clifford, B.M. Macduff, D.M. Anderson, S.D. Gimpel, T. Davis-Smith, and C.R.
Maliszewski. 1992. Nature 357:80.

  1. Quick-Filters:
  2. Cited (9)
  3. Overnight Delivery (1)

Items 1 to 20 of 133 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD154 / CD40L antibody

CD154 / CD40L Mouse IgG1 MK13A4 Azide Free Hu F, FN
0.1 mg / €570.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Mouse IgG1 MK13A4 Biotin Hu F
100 Tests / €480.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Mouse IgG1 MK13A4 FITC Hu F
100 Tests / €470.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Mouse 24-31 Purified Hu F
25 µg / €120.00
  OriGene Technologies, Inc.

CD154 / CD40L antibody

WB Suggested Anti-CD40LG Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD154 / CD40L antibody

CD40L ELISA with MK13A4 Capture (TA600027) and MK13A4 Detection (TA700027) Antibodies. Substrate used: Recombinant Human CD40L (E. Coli expressed Peptide) Mouse IgG1 OTIMK13A4 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD154 / CD40L antibody

CD40L ELISA with MK13A4 Capture (TA600027) and MK13A4 Detection (TA700027) Antibodies. Substrate used: Recombinant Human CD40L (E. Coli expressed Peptide) Mouse IgG1 OTIMK13A4 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

CD154 / CD40L antibody

CD154 / CD40L Hamster IgG MR1 Biotin Ms F
0.5 mg / €370.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

Immunofluoscent Staining: Partially purified spleen T cells from BALB/c mice were incubated with either Hamster IgG or plate-bound Hamster anti-Mouse CD3e (clone 145-2C11) for 7 hours at 37°C. The cells were then harvested, double-stained with anti-CD154/CD40L-FITC and anti-CD4+8-PE, and analyzed by flow cytometry. Amount Used: 2 µg/10e6 cells. Hamster IgG MR1 FITC Ms F
0.5 mg / €370.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Hamster IgG MR1 Low Endotoxin Ms F, FN, IP
0.5 mg / €360.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Hamster IgG MR1 Purified Ms F, FN, IP
0.5 mg / €290.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Hamster IgG MR1 PE Ms F
0.1 mg / €300.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Mouse IgG1 24-31 Purified Hu, Mky FN, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

Flow cytometric analysis of CD154 expression on transfectant. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26429AF-N to the cells. Mouse IgG1 5F3 Azide Free Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Mouse IgG1 5F3 FITC Hu F
50 Tests / €380.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Mouse IgG1 24-31 Low Endotoxin Hu, Prim F, FN, ICC/IF
0.1 mg / €210.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Mouse IgG1 24-31 Purified Hu, Prim F, FN, ICC/IF
0.1 mg / €210.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

CD154 / CD40L Mouse IgG1 24-31 PE Hu, Prim F
100 Tests / €290.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

Immunohistochemistry analysis on Frozen Section (Acetone fixed) of human colic mucosa using CD154 / CD40L  (Clone B-B29 ) antibody Cat.-No. AM31238AF-N (diluted at 1/100. Bbackground coloration by hematoxylin. Mouse IgG2a B-B29 Azide Free Hu C, F
0.2 mg / €270.00
  Acris Antibodies GmbH

CD154 / CD40L antibody

A typical staining pattern with the B-B29 monoclonal antibody of CD40L transfected 3T6 cell line Mouse IgG2a B-B29 FITC Hu F
1ml (100T) / €270.00
  Acris Antibodies GmbH

Items 1 to 20 of 133 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  • LinkedIn