CD156b / ADAM17 antibody

Principal name

CD156b / ADAM17 antibody

Alternative names for CD156b / ADAM17 antibody

CSVP, TACE, Disintegrin and metalloproteinase domain-containing protein 17, TNF-alpha-converting enzyme, TNF-alpha convertase, Snake venom-like protease

SwissProt ID

O77636 (Pig), P78536 (Human), Q9Z0F8 (Mouse), Q9Z1K9 (Rat)

Gene ID

6868 (ADAM17)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Radioimmunoassay (R), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD156b / ADAM17 antibody

ADAM17, also known as TNF alpha Convertase, or TACE, was first cloned from human epithelial cells. The ADAMs proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane and Cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". ADAM17 contains a cleavage site for prohormone convertases (furin), and the majority of ADAM17 is secreted as activated enzyme, cleaved at the furin site. ADAM17 is known to associate with MAD2 via the Cytoplasmic domain, implying cellular regulation pathways. ADAM17 is expressed in a wide range of cells in culture. Three human ADAM17 sequences encode proteins with different cytoplasmic domains, as is the case for other ADAMs proteases. The 824 amino acid sequence encodes a 92,960 Dalton protein, the 807 AA sequence 91,003 Dalton, and the 694 AA sequence 78,543 Dalton predicted MW. Glycosylation and other post translational modifications increase the apparent molecular weight on PAGE gels. Mouse and rat ADAM17 sequences are both 827 AA, predicting 93,073 and 93,017 Dalton proteins, respectively. Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.

General readings

Black RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, Castner BJ, Stocking KL, Reddy P, Srinivasan S, Nelson N, Boiani N, Schooley KA, Gerhart M, Davis R, Fitzner JN, Johnson RS, Paxton RJ, March CJ, Cerretti DP A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature. 1997 Feb 20;385(6618):729-33 PMID: 9034190

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD156b / ADAM17 antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 1F6 Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CD156b / ADAM17 (C-term) antibody

TA302775 staining (0.2µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
20 µg / €200.00
  Acris Antibodies GmbH

CD156b / ADAM17 (C-term) antibody

TA302775 staining (0.2µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD156b / ADAM17 (C-term) antibody

Western blot analysis of TACE in HeLa and Jurkat whole cell lysate in the absence or presence of blocking peptide (Cat. No. 1131P) with TACE antibody at 1:500 dilution. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD156b / ADAM17 antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid and lung cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ADAM17 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD156b / ADAM17 antibody

Western blot analysis of extracts from rat liver cells, using ADAM 17 (Cleaved-Arg215) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD156b / ADAM17 (C-term) antibody

Figure 1. Western blot analysis using anti-TACE (CT) antibody at 1 µg/ml on HeLa (1,3) an Jurkat (2,4) whole cell lysate in the absense (1,2) and presense (3,4) of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CD156b / ADAM17 antibody

Figure 1. Staining TACE in Skeletal Muscle by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD156b / ADAM17 (C-term) antibody

Skeletal muscle: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD156b / ADAM17 (N-term) antibody

Western blot analysis of ADAM17 Antibody (N-term)in CEM cell line lysates (35 µg/lane). ADAM17 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Aff - Purified Hu WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

CD156b / ADAM17 (C-term) antibody

Western blot analysis of ADAM17 Antibody (N-term) in Jurkat cell line lysates (35 µg/lane). ADAM17 (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD156b / ADAM17 Control Peptide

CD156b / ADAM17
50 µg / €160.00
  Acris Antibodies GmbH

CD156b / ADAM17 antibody

Western blot analysis of CD156b (TACE) in HeLa (1,3) and Jurkat (2,4) whole cell lysate in the absence (1,2) or presence (3,4) of blocking peptide with SP1302. Rabbit Aff - Purified Hu WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD156b / ADAM17 antibody

Immunoperoxidase of monoclonal antibody to ADAM17 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse IgG2b 1F6 Purified Hu E, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD156b / ADAM17 antibody

CD156b / ADAM17 Mouse IgG2b 1F6 Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

CD156b / ADAM17 antibody

Western blot analysis of ADAM17 polyclonal antibody ( Cat # PAB1888 ) in CEM cell line lysates ( 35 µg/lane ). ADAM17 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ). Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CD156b / ADAM17 antibody

Western blot analysis of ADAM17 polyclonal antibody ( Cat # PAB1889 ) in Jurkat cell line lysates ( 35 µg/lane ) . ADAM17 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

CD156b / ADAM17 antibody

CD156b / ADAM17 Rabbit Hu E, R
0.4 mg / €390.00
  Abnova Taiwan Corp.

CD156b / ADAM17 antibody

CD156b / ADAM17 Rabbit Hu E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

CD156b / ADAM17 antibody

CD156b / ADAM17 Rabbit Hu E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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