NKAT1, Killer cell immunoglobulin-like receptor 2DL, 1MHC class I NK cell receptor, Natural killer-associated transcript 1, p58 natural killer cell receptor clones CL-42/47.11, p58.1 MHC class-I-specific NK receptor, CD158 antigen-like family member A
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of CD158a / KIR2DL1 antibody
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the "framework" loci that is present on all haplotypes. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Western blot analysis: Recombinant Human protein KIR2DL1, KIR2DL3, KIR2DS4 and KIR2DL4 (each 50 ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SM6033 anti-Human KIR2DL1 antibody (1/500). Proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot analysis of KIR2DL1 expression in transfected 293T cell line (H00003802-T01) by KIR2DL1 MaxPab polyclonal antibody.Lane 1: KIR2DL1 transfected lysate(41.14 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of recombinant human protein KIR2DL1 , KIR2DL3 , KIR2DS4 and KIR2DL4 ( each 50 ng per well ) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with KIR2DL1 monoclonal antibody , clone 2F9 ( 1 : 500 ) ( Cat # MAB1078 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot analysis of KIR2DL1 expression in transfected 293T cell line (H00003802-T02) by KIR2DL1 MaxPab polyclonal antibody.Lane 1: KIR2DL1 transfected lysate(41.40 KDa).Lane 2: Non-transfected lysate.
Recombinant human protein kIR2DL1, kIR2DL3, kIR2DS4 and kIR2DL4 (each 50ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human kIR2DL1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody co
Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Anti-CD158 Polyclonal Antibody, Unconjugated (bs-2643R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.