CD162 / PSGL1 antibody

Principal name

CD162 / PSGL1 antibody

Alternative names for CD162 / PSGL1 antibody

PSGL-1, Selectin P ligand, SELPLG, P-selectin glycoprotein ligand 1

SwissProt ID

Q14242 (Human), Q62170 (Mouse)

Gene ID

6404 (SELPLG), 20345

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD162 / PSGL1 antibody

Cutaneous Lymphocyte-assiciated Antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb HECA-452. It is expressed on T lymphocytes in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells, as well as on monocytes, granulocytes and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid- and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on majority of peripheral blood leukocytes. CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering and rolling. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA, and is suitable for staining formalin-fixed paraffin embedded tissue sections. CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

General readings

Clone PL2 is used in these references:
1. Moore, K.L., et al. J. Cell Biol. 128, 661-671 (1995)
2. Li, F., et al. J. Biol. Chem. 271, 3255-3264 (1996)
3. Laszik, Z., et al. Blood 88, 3010-3021 (1996)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD162 / PSGL1 antibody

Western blot analysis of extracts of Jurkat cell lines, using SELPLG antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD162 / PSGL1 antibody

WB Suggested Anti-SELPLG Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Heart Rabbit IgG Purified Bov, Can, GP, Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD162 / PSGL1 antibody

WB Suggested Anti-SELPLG Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Heart Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD162 / PSGL1 antibody

Surface staining of human peripheral blood cells with anti-human CD162 (clone TC2). Mouse IgG1 TC2 APC Hu
100 Tests / €290.00
  Acris Antibodies GmbH

CD162 / PSGL1 antibody

Surface staining of human peripheral blood cells with anti-Human CD162 antibody (clone TC2). Mouse IgG1 TC2 FITC Hu F
100 Tests / €260.00
  Acris Antibodies GmbH

CD162 / PSGL1 antibody

Surface staining of human peripheral blood cells with anti-human CD162 (clone TC2). Mouse IgG1 TC2 Aff - Purified Hu F, P
0.1 mg / €220.00
  Acris Antibodies GmbH

CD162 / PSGL1 antibody

Surface staining of human peripheral blood cells with anti-human CD162 (clone TC2). Mouse IgG1 TC2 PE Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD162 / PSGL1 antibody

IHC Image of AM20569PU-N:
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rat IgM HECA-452 Aff - Purified Hu, Ms F, P
50 µg / €470.00
  Acris Antibodies GmbH

CD162 / PSGL1 (C-term) antibody

Western blot analysis of SELPLG Antibody (C-term) Cat.-No AP53840PU-N in Jurkat cell line lysates (35ug/lane). This demonstrates the SELPLG antibody detected the SELPLG protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD162 / PSGL1 antibody

CD162 / PSGL1 Mouse IgG1 PL1 FITC Hu F
1ml (50 Tests) / €300.00
  Acris Antibodies GmbH

CD162 / PSGL1 antibody

CD162 / PSGL1 Mouse IgG1 3E2.25.5 (PL1) Purified Hu C, P, WB
0.2 mg / €500.00
  Acris Antibodies GmbH

CD162 / PSGL1 antibody

CD162 / PSGL1 Mouse IgG1 3E2.25.5 (PL1) Purified Hu F, FN
25 µg / €200.00
  Acris Antibodies GmbH

CD162 / PSGL1 antibody

CD162 / PSGL1 Mouse IgG1 PL2 FITC Hu F
1ml (50 Tests) / €250.00
  Acris Antibodies GmbH

CD162 / PSGL1 antibody

CD162 / PSGL1 Mouse IgG1 5D8.8.12 (PL2) Hu C, F, P, WB
0.2 mg / €640.00
  Abnova Taiwan Corp.

CD162 / PSGL1 antibody

CD162 / PSGL1 Mouse IgG1 3E2.25.5 Hu C, P, WB
0.2 mg / €990.00
  Abnova Taiwan Corp.

CD162 / PSGL1 antibody

Western blot analysis of Selplg expression in Jurkat cell lysate. Rabbit Hu, Ms, Rt WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

CD162 / PSGL1 antibody

Flow Cytometry: CD162 Antibody (KPL-1) [NB100-78111] - Human peripheral blood lymphocytes stained with purified KPL-1, followed by anti-mouse IgG FITC Mouse IgG1 KPL-1 Aff - Purified Hu, Mky F
0.1 mg / €320.00
  Novus Biologicals Inc.

CD162 / PSGL1 antibody

CD162 / PSGL1 Mouse IgG1 TB5 Aff - Purified Hu F, P
0.1 mg / €340.00
  Novus Biologicals Inc.

CD162 / PSGL1 antibody

Western Blot: CD162 Antibody (EPR1967Y) [NBP1-40451] - Western blot analysis on (A) Jurkat cell lysate and (B) 5ng of recombinant protein using anti-PSGL1, dilution 1:500. Rabbit IgG EPR1967Y Hu ICC/IF, IP, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

CD162 / PSGL1 antibody

CD162 / PSGL1 Mouse IgG1 TC2 FITC Hu F
100 Tests / €360.00
  Novus Biologicals Inc.

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