CD163 antibody

Principal name

CD163 antibody

Alternative names for CD163 antibody

M130, Hemoglobin scavenger receptor, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

SwissProt ID

Q2VL90 (Pig), Q2VLG6 (Canfa), Q2VLH6 (Mouse), Q86VB7 (Human)

Gene ID

9332 (CD163), 397031

Available reactivities

Hu (Human), Mky (Monkey), Rt (Rat), Sh (Sheep), Por (Porcine), Rhesus Mky (Rhesus Monkey), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Functional assay (FN)

Background of CD163 antibody

CD163 is a 130 kDa membrane glycoprotein. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 is very useful for macrophage phenotyping. Positive staining can be seen in the skin (histiocytes), gut, Kupffer cells, few aveolar macrophages, main population of macrophages in the placenta, varying degrees of macrophages in inflammed tissue including tumorous tissue depending on the inflammatory stage. Red pulp, not white pulp, macrophages in the spleen and cortical macrophages of the thymus are detected.

General readings

1. Dijkstra CD, Döpp EA, Joling P, Kraal G. The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 1985 Mar;54(3):589-99. PubMed PMID: 3882559.
2. Beelen, R.H.J. et al. (1987) Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: Expression of recognized antigens in different stages of differentiation. Transplant. Proc. 3: 3166-3170.
3. Barbé E, Damoiseaux JG, Döpp EA, Dijkstra CD. Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2. Immunobiology. 1990 Dec;182(1):88-99. PubMed PMID: 2098324.
4. Dijkstra CD, Damoiseaux JG. Macrophage heterogeneity established by immunocytochemistry. Prog Histochem Cytochem. 1993;27(2):1-65. PubMed PMID: 8248543.
5. Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 1995 Mar;43(3):313-20. PubMed PMID: 7868861.
6. Polfliet, M.M.J. et al. (2002) Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators. PhD Thesis. Vrije University, Amsterdam.
7. Deng X, Wang L, Elm MS, Gabazadeh D, Diorio GJ, Eagon PK, et al. Chronic alcohol consumption accelerates fibrosis in response to cerulein-induced pancreatitis in rats. Am J Pathol. 2005 Jan;166(1):93-106. PubMed PMID: 15632003.
8. Dorval-Coiffec I, Delcros JG, Hakovirta H, Toppari J, Jégou B, Piquet-Pellorce C. Identification of the leukemia inhibitory factor cell targets within the rat testis. Biol Reprod. 2005 Mar;72(3):602-11. Epub 2004 Nov 10. PubMed PMID: 15537862.
9. Fujita, E. et al. (2010) Statin Attenuates Experimental Anti-Glomerular Basement Membrane Glomerulonephritis Together with the Augmentation of Alternatively Activated Macrophages. Am J Pathol. Aug 9. [Epub ahead of print].
10. Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, et al. NK cell depletion delays corneal allograft rejection in baby rats. Mol Vis. 2010 Oct 2;16:1928-35. PubMed PMID: 21031017.
11. Baker, S.C. et al. (2011) Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(e-caprolactone) Scaffold. Macromol Biosci. doi: 10.1002/mabi.201000415.
12. Bedi A, Kovacevic D, Fox AJ, Imhauser CW, Stasiak M, Packer J, et al. Effect of early and delayed mechanical loading on tendon-to-bone healing after anterior cruciate ligament reconstruction. J Bone Joint Surg Am. 2010 Oct 20;92(14):2387-401. doi: 10.2106/JBJS.I.01270. PubMed PMID: 20962189.
13. Banerjee S, Figueiredo FC, Easty DL, Dick AD, Nicholls SM. Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats. Br J Ophthalmol. 2003 Dec;87(12):1515-22. PubMed PMID: 14660464.
14. Moghaddami M, Mayrhofer G, Cleland LG. MHC class II compartment, endocytosis and phagocytic activity of macrophages and putative dendritic cells isolated from normal tissues rich in synovium. Int Immunol. 2005 Aug;17(8):1117-30. Epub 2005 Jul 18. PubMed PMID: 16027140.
15. Wójcik M, Ramadori P, Blaschke M, Sultan S, Khan S, Malik IA, et al. Immunodetection of cyclooxygenase-2 (COX-2) is restricted to tissue macrophages in normal rat liver and to recruited mononuclear phagocytes in liver injury and cholangiocarcinoma. Histochem Cell Biol. 2012 Feb;137(2):217-33. doi: 10.1007/s00418-011-0889-9. Epub 2011 Dec 1. PubMed PMID: 22131058.
16. Kawakami AP, Sato C, Cardoso TN, Bonamin LV. Inflammatory Process Modulation by Homeopathic Arnica montana 6CH: The Role of Individual Variation. Evid Based Complement Alternat Med. 2011;2011:917541. doi: 10.1155/2011/917541. Epub 2011 Jan 19. PubMed PMID: 21318109.
17. Kajita M, Murata T, Horiguchi K, Iizuka M, Hori M, Ozaki H. iNOS expression in vascular resident macrophages contributes to circulatory dysfunction of splanchnic vascular smooth muscle contractions in portal hypertensive rats. Am J Physiol Heart Circ Physiol. 2011 Mar;300(3):H1021-31. doi: 10.1152/ajpheart.00563.2009. Epub 2010 Dec 30. PubMed PMID: 21193589.
18. Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. The identification of CD163 expressing phagocytic chondrocytes in joint cartilage and its novel scavenger role in cartilage degradation. PLoS One. 2013;8(1):e53312. doi: 10.1371/journal.pone.0053312. Epub 2013 Jan 11. PubMed PMID: 23326413.
19. Fujii, Y. et al. (2013) Effect of enzymatically modified isoquercitrin on preneoplastic liver cell lesions induced by thioacetamide promotion in a two-stage hepatocarcinogenesis model using rats. Toxicology. pii: S0300-483X(13)00006-1.
20. Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, Fossum S, et al. Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells. PLoS One. 2013;8(2):e57406. doi: 10.1371/journal.pone.0057406. Epub 2013 Feb 28. PubMed PMID: 23468983.
21. Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor. Blood, 15:5223-5229.
22. Park, E.S. et al. (2014) Establishment of a Rat Model for Canine Necrotizing Meningoencephalitis (NME). Vet Pathol. Jan 21 [Epub ahead of print]
23. Keitel V, Donner M, Winandy S, Kubitz R, Häussinger D. Expression and function of the bile acid receptor TGR5 in Kupffer cells. Biochem Biophys Res Commun. 2008 Jul 18;372(1):78-84. doi: 10.1016/j.bbrc.2008.04.171. Epub 2008 May 9. PubMed PMID: 18468513. 
24. Hozumi, Y. et al. (2015) Expression and localization of the diacylglycerol kinase family and of phosphoinositide signaling molecules in adrenal gland. Cell Tissue Res. May 24. [Epub ahead of print] 
25. Fernandez-Bustamante, A. et al. (2015) Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats. PLoS One. 10 (7): e0130764. 
26. Adamo, H.H. et al. (2015) Adaptive (TINT) Changes in the Tumor Bearing Organ Are Related to Prostate Tumor Size and Aggressiveness. PLoS One. 10 (11): e0141601

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD163 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506380) Mouse IgG1 OTI2G12 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD163 antibody

Staining of ED2 antibody on Rat spleen (frozen section) Cat.-No. BM4001 Mouse IgG1 ED2 Purified Rt C, F, ICC/IF, IP, P, WB
0.25 mg / €620.00
  Acris Antibodies GmbH

CD163 antibody

Staining of ED2 antibody on Rat spleen (frozen section) Cat.-No. BM4001 Mouse IgG1 ED2 Purified Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

CD163 antibody

BM4041 CD163 antibody staining of Paraffin Human Tonsil Section (Clone 5C6-FAT) Mouse IgG1 5C6-FAT Purified Hu C, E, F, P, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

CD163 antibody

BM4041B CD163 antibody staining of Frozen Human Placenta Section (Clone 5C6-FAT) Mouse IgG1 5C6-FAT Biotin Hu C, F, P
0.1 mg / €520.00
  Acris Antibodies GmbH

CD163 antibody

CD163 Mouse IgG1 5C6-FAT FITC Hu F
0.1 mg / €530.00
  Acris Antibodies GmbH

CD163 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506382) Mouse IgG2a OTI2B12 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD163 antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506383) Mouse IgG1 OTI1D5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD163 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506386) Mouse IgG2a OTI3B4 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD163 antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506388) Mouse IgG2a OTI2F8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD163 antibody

Immunofluorescent staining of HeLa cells using anti-CD163 mouse monoclonal antibody (TA506390). Mouse IgG1 OTI2C1 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD163 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506391) Mouse IgG1 OTI1B4 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD163 antibody

Immunohistochemistry on frozen sections: Human placenta, stained with AP26434PU-N. Rabbit Purified Hu, Por C, WB
1 mg / €480.00
  Acris Antibodies GmbH

CD163 antibody

Paraffin Sections of Human Tonsils using 5C6-FAT Mouse IgG1 5C6-FAT Purified Hu C, E, F, P, WB
1 mg / €1,020.00
  Acris Antibodies GmbH

CD163 (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human placenta (4 µm) stained with anti-CD163 antibody (DB 045) shows strong positive cytoplasmic immunostaining of monocytes and macrophages. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG K20-T Aff - Purified Hu P
0.1 ml / €280.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD163 (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human placenta (4 µm) stained with anti-CD163 antibody (DB 045) shows strong positive cytoplasmic immunostaining of monocytes and macrophages. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG K20-T Aff - Purified Hu P
0.2 ml / €370.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD163 (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human placenta (4 µm) stained with anti-CD163 antibody (DB 045) shows strong positive cytoplasmic immunostaining of monocytes and macrophages. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG K20-T Aff - Purified Hu P
0.5 ml / €470.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD163 (N-term) antibody

Formalin-Fixed, Paraffin-Embedded Human placenta (4 µm) stained with anti-CD163 antibody (DB 045) shows strong positive cytoplasmic immunostaining of monocytes and macrophages. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG K20-T Aff - Purified Hu P
1 ml / €720.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD163 antibody

AM21062PU CD163 antibody staining of Human Lung. Rabbit IgG SP96 Purified Hu P
0.5 ml / €510.00
  Acris Antibodies GmbH

CD163 antibody

AM21062PU CD163 antibody staining of Human Lung. Rabbit IgG SP96 Purified Hu P
1 ml / €730.00
  Acris Antibodies GmbH

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