CD170 / SIGLEC5 antibody

Principal name

CD170 / SIGLEC5 antibody

Alternative names for CD170 / SIGLEC5 antibody

CD33L2, OBBP2, OB-binding protein 2, Siglec-5, Sialic acid-binding Ig-like lectin 5, Obesity-binding protein 2, CD33 antigen-like 2

SwissProt ID

O15389 (Human), Q920G3 (Mouse)

Gene ID

8778 (SIGLEC5), 233186 (Siglec5)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit, Rat, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD170 / SIGLEC5 antibody

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have one or more cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIM). SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and four C2-set Ig-like domains, five potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8. Expression was also detected on monocytes and a CD16-positive/CD56-negative natural killer-like lymphocyte population. After sialidase treatment, which is necessary for unmasking the sialic acid-binding site on SIGLECs interacting with cell surface sialic acids, cells expressing SIGLEC10 bound to red blood cells. Immunoprecipitation analysis indicated expression of a 100- to 120-kD monomeric protein, higher than the predicted molecular mass, suggesting that SIGLEC10 is glycosylated.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD170 / SIGLEC5 antibody

  Mouse monoclonal Anti-SIGLEC5 Clone 1A5  
CD170 / SIGLEC5 Mouse IgG1 1A5 Purified Hu E, F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD170 / SIGLEC5 antibody

Immunoprecipitation of mouse SiglecE from 293T cells (1 and 2) and mouse SiglecE transfected cells (2 and 4) with rat IgG2b (1 and 2) or AM26632AF-N (3 and 4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with HRP-Streptavidin. Rat IgG2b 8D2 Azide Free Ms F, IP
0.1 mg / €350.00
  Acris Antibodies GmbH

CD170 / SIGLEC5 antibody

Flow cytometric analysis mouse SiglecE expression on mouse splenocytes. The staining intensity of rat IgG2b at 50 μg/ml (left) or AM26632FC-N at 50 μg/ml (right) are shown in the horizontal axis with CD11c staining on the vertical axis. Rat IgG2b 8D2 FITC Ms F
50 µg / €370.00
  Acris Antibodies GmbH

CD170 / SIGLEC5 (N-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SIGLEC5 Antibody (N-term) (AP11626PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD170 / SIGLEC5 (C-term) antibody

The anti-Siglec5 C-term (AP11627PU-N) is used in Western blot to detect Siglec5 in mouse liver tissue lysate. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD170 / SIGLEC5 (N-term) antibody

Western blot analysis of CD170 / SIGLEC5 Antibody Cat.-No AP53917PU-N in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SIGLEC5 antibody detected the SIGLEC5 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Siglec5 - mouse gene knockout kit via CRISPR

Siglec5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Siglec5 - mouse gene knockout kit via CRISPR

Siglec5 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Siglec5 - mouse gene knockout kit via CRISPR

Siglec5 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Siglec5 - mouse gene knockout kit via CRISPR

Siglec5 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD170 / SIGLEC5

  Recombinant protein of human sialic acid binding Ig-like lectin 5 (SIGLEC5)  
CD170 / SIGLEC5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

SIGLEC5 overexpression lysate

SIGLEC5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SIGLEC5 (mGFP-tagged) - Human sialic acid binding Ig-like lectin 5 (SIGLEC5), 200 uL, >10^7 TU/mL

Lenti ORF particles, SIGLEC5 (mGFP-tagged) - Human sialic acid binding Ig-like lectin 5 (SIGLEC5), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, SIGLEC5 (Myc-DDK tagged) - Human sialic acid binding Ig-like lectin 5 (SIGLEC5) , 200 uL, >10^7 TU/mL

Lenti ORF particles, SIGLEC5 (Myc-DDK tagged) - Human sialic acid binding Ig-like lectin 5 (SIGLEC5) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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