CD178 / Fas Ligand antibody

Principal name

CD178 / Fas Ligand antibody

Alternative names for CD178 / Fas Ligand antibody

FASLG, APT1LG1, FASL, TNFSF6, CD95L protein, APTL, Tumor necrosis factor ligand superfamily member 6, Fas antigen ligand, Apoptosis antigen ligand

SwissProt ID

P36940 (Rat), P41047 (Mouse), P48023 (Human), P63308 (Macfa), Q861W5 (Felca), Q9BEA8 (Pig)

Gene ID

356 (FASLG), 14103 (Fasl), 25385 (Faslg)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Rb (Rabbit), Bov (Bovine)

Available hosts

Rabbit, Mouse, Hamster, Goat, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Functional assay (FN), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Neutralisation (NEUT), EPair for Elisa (EPair), ELISA (detection) (E(detection))

Background of CD178 / Fas Ligand antibody

Fas ligand (FasL/CD178/CD95L) is a type II-membrane protein, whose N terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas, also termed Apo-1 or CD95, is a cell-surface type I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. It is known that engagement of Fas by FasL results in apoptotic cell death. Binding of a trimeric FasL to Fas induces trimerization of Fas, and FADD/MORT1 binds to the trimerized Fas cytoplasmic region through the interaction of the respective death domains. Caspase-8 is then recruited to FADD/MORT1 through binding of each death effector domains (DED), which in turn may induce self-activation of the protease domain and eventually leads cell to apoptosis. FasL is predominantly expressed in activated T lymphocytes and Natural Killer (NK) cells, although it is expressed in the tissues of the immune-privilege sites such as the testis and eye. Furthermore, a variety of cancers such as melanomas were found to express FasL in addition to Fas. On the other hand, Fas is expressed in various tissues with abundant expression in the thymus, liver, heart, and kidney. It is reported that Fas/FasL system is involved in the deletion of activated or autoreactive T and B cells. Mature T cells of normal mice are known to die, after they accomplish their tasks. On the contrary, mature T cells from lpr and gld mice, which are deficient of Fas and FasL respectively, do not die after activation, and activated cells accumulate in the lymph nodes and spleens of these mice. Moreover T cell hybridomas activated in the presence of Fas-neutralizing molecule, do not die. In addition to T cells, the Fas-deficient mice accumulate B cells and have elevated levels of immunoglobulins (Ig) of various classes that include anti-ssDNA and anti-dsDNA antibodies. FasL can be used as an immune-suppressive agent. Rejection of transplants is mediated by activated T cells that may express functional Fas. If a transplanted tissue is engineered to express FasL or is co-transplanted with FasL expressing cells, the transplant may be tolerated. Also Fas/ FasL system has been shown to play a role in human diseases such as fulminant hepatitis, AIDS, cancers and other diseases involving CTL-induced tissue destruction.

General readings

General / background references:Takahashi T et al. Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand. Cell 76:969-76 (1994). PubMed PMID: 7511063
Cifone MG et al. Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase. J Exp Med 180:1547-52 (1994). PubMed PMID: 7523573
Oehm A et al. Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen. J Biol Chem 267:10709-15 (1992). PubMed PMID: 1375228

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD178 / Fas Ligand antibody

Human prostate stained with anti-Fas ligand antibody Rabbit Aff - Purified Hu, Ms, Por, Rb, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

Human prostate stained with anti-Fas ligand antibody Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €520.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

Human prostate stained with anti-Fas ligand antibody Rabbit Aff - Purified Hu, Ms, Rt P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

Western blot analysis of extracts from 293 cellls, using FAS ligand antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD178 / Fas Ligand antibody

CD178 / Fas Ligand Mouse NOK-1 Purified Hu F, IP
25 µg / €120.00
  OriGene Technologies, Inc.

CD178 / Fas Ligand antibody

This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma.  The recommended concentration is 0.75 ug/mL - 2.5 ug/mL with an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with a AB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Goat IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD178 / Fas Ligand antibody

Host: Rabbit; Target Name: TNFSF6; Sample Tissue: Hela Cell; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD178 / Fas Ligand antibody

Western blot analysis on 293 cell lysate using FASL Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD178 / Fas Ligand antibody

Western blot analysis of FAS ligand expression in 293 cell extract Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD178 / Fas Ligand antibody

ELISA: Fas Ligand Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD178 / Fas Ligand antibody

Immunohistochemical staining of human tonsil shows distinct cytoplasmic and membranous positivity in a subset of lymphoid cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD178 / Fas Ligand antibody

Western blot Analysis of FasL expression in 293 Cell Lysate. Mouse IgG1 I-6060 Purified Hu C, IP, P, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

CD178 / Fas Ligand Hamster IgG 4A5 Azide Free Hu E, F, FN
0.1 mg / €670.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

Staining of Mouse CD178 transfected cells with FITC conjugated Hamster anti-Mouse CD178 antibody AM20860FC-N Hamster IgG MFL4 FITC Ms, Rt F
0.1 mg / €270.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

Staining of Mouse CD178 transfected cells with FITC conjugated Hamster anti-Mouse CD178 antibody AM20860FC-N Hamster IgG MFL4 FITC Ms, Rt F
25 µg / €190.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

CD178 / Fas Ligand Hamster IgG MFL4 Low Endotoxin Ms, Rt F, FN
0.5 mg / €540.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

CD178 / Fas Ligand Hamster IgG MFL4 Purified Ms, Rt F
0.25 mg / €400.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

CD178 / Fas Ligand Hamster IgG MFL4 Purified Ms, Rt F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

CD178 / Fas Ligand Hamster IgG MFL4 Purified Ms, Rt F
25 µg / €220.00
  Acris Antibodies GmbH

CD178 / Fas Ligand antibody

Staining of Mouse CD178 transfected cells with PE conjugated Hamster anti-Mouse CD178 antibody AM20860RP-N Hamster IgG MFL4 PE Ms, Rt F
100 Tests / €430.00
  Acris Antibodies GmbH

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