CD18 / ITGB2 antibody

Principal name

CD18 / ITGB2 antibody

Alternative names for CD18 / ITGB2 antibody

Integrin beta-2, MFI7, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Complement receptor C3 subunit beta

SwissProt ID

P05107 (Human), P11835 (Mouse), P32592 (Bovin), P53714 (Pig), Q09062 (Xenla), Q6ECI6 (Sheep), Q9JI30 (Rat)

Gene ID

3689 (ITGB2), 16414 (Itgb2), 25021 (Itgam), 281877, 396943

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Functional assay (FN), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair)

Background of CD18 / ITGB2 antibody

In the immune system, integrins have essential roles in leukocyte trafficking and function. These include immune cell attachment to endothelial and antigen-presenting cells, cytotoxicity, and extravasation into tissues. Integrin adhesion receptors transduce sigls that control complex cell functions which require the regulation of gene expression, such as proliferation, differentiation and survival. Their intracellular domain has no catalytic function, indicating that interaction with other transducing molecules is crucial for integrin-mediated sigling. JAB1 (Jun activation domain-binding protein 1), a coactivator of the c-Jun transcription factor, has been identified as a protein that interacts with the cytoplasmic domain of the beta2 subunit of the alphaL/beta2 integrin LFA-1. Patients with leukocyte adhesion molecule (CD11/CD18, beta 2 integrins) deficiency have structural defects in the common beta subunit (CD18), which prevent heterodimer formation and normal cell surface expression of these receptors, leading to life-threatening bacterial infections.

General readings

1. Gao JX, Issekutz AC: Mac-1 (CD11b/CD18) is the predominant beta 2 (CD18) integrin mediating human neutrophil migration through synovial and dermal fibroblast barriers. Immunology. 1996 Jul;88(3):463-70.
2. Shang XZ, Issekutz AC: Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol. 1998 Jun;28(6):1970-9.
3. Kess D, Peters T, Zamek J, Wickenhauser C, Tawadros S, Loser K, Varga G, Grabbe S, Nischt R, Sunderkötter C, Müller W, Krieg T, Scharffetter-Kochanek K: CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasis. J Immunol. 2003 Dec 1;171(11):5697-706.
4. Solovjov DA, Pluskota E, Plow EF: Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2. J Biol Chem. 2005 Jan 14;280(2):1336-45.
5. Peters T, Sindrilaru A, Wang H, Oreshkova T, Renkl AC, Kess D, Scharffetter-Kochanek K: CD18 in monogenic and polygenic inflammatory processes of the skin. J Investig Dermatol Symp Proc. 2006 Sep;11(1):7-15.
6. Dassanayake RP, Maheswaran SK, Srikumaran S: Monomeric expression of bovine beta2-integrin subunits reveals their role in Mannheimia haemolytica leukotoxin-induced biological effects. Infect Immun. 2007 Oct;75(10):5004-10.
7. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
8. Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V: Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun. 2000 Aug 28;275(2):295-9.
9. Drbal K, Angelisova P, Cerny J, Hilgert I, Horejsi V: A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins. Immunobiology. 2001 May;203(4):687-98.
10. Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6.
11. Aubert M, Yoon M, Sloan DD, Spear PG, Jerome KR: The virological synapse facilitates herpes simplex virus entry into T cells. J Virol. 2009 Jun;83(12):6171-83.
12. Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61.
13. Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD18 / ITGB2 antibody

Staining of mouse splenocytes with Anti-Mouse CD18 PE.  Autofluorescence is shown via open histogram.  Total viable cells were used for analysis. Rat M18/2 Purified Ms F, IP, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD18 / ITGB2 antibody

Western blot analysis of extracts of THP-1 cell lines, using ITGB2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD18 / ITGB2 (86-99) antibody

TA334177 (0.3µg/ml) staining of K562 (A) and U937 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD18 / ITGB2 antibody

  Rabbit Polyclonal Anti-ITGB2 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T cells, K562 cells, Primary antibody: (ITGB2 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD18 / ITGB2 antibody

Human peripheral blood lymphocytes stained with purified TS1/18, followed by anti-mouse IgGs FITC Mouse TS1/18 Purified Hu F
0.1 mg / €248.00
  OriGene Technologies, Inc.

CD18 / ITGB2 antibody

C57BL/6 mouse splenocytes stained with purified M18/2, followed by anti-rat IgG FITC Rat M18/2 Purified Ms F, IP, P, WB
50 µg / €248.00
  OriGene Technologies, Inc.

CD18 / ITGB2 antibody

CD18 / ITGB2 Mouse IgG1 24 Purified Hu F, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD18 / ITGB2 antibody

CD18 / ITGB2 Mouse IgG1 Bu86 FITC Hu F
0.1 mg / €390.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

CD18 / ITGB2 Mouse IgG1 Bu86 FITC Hu F
25 µg / €190.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

CD18 / ITGB2 Mouse IgG1 Bu86 Purified Hu F
0.2 mg / €430.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

CD18 / ITGB2 Mouse IgG1 PNK-I FITC Por F
0.1 mg / €530.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

Staining of porcine eripheral blood granulocytes with MOUSE ANTI PORCINE CD18a Mouse IgG1 PNK-I Purified Por F, IP
0.2 mg / €530.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

CD18 / ITGB2 Mouse IgG1 PNK-I PE Por F
100 Tests / €330.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

CD18 / ITGB2 Mouse IgG1 3EB Biotin Hu F
100 Tests / €270.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

Figure 1. Peripheral Blood Lymphocytes Stained with AM05179FC-N antibody. Mouse IgG1 3EB FITC Hu F
100 Tests / €270.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

CD18 / ITGB2 Mouse IgG1 3EB Purified Hu F
0.1 mg / €250.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

CD18 / ITGB2 Mouse IgG1 3EB PE Hu F
100 Tests / €300.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

CD18 / ITGB2 Rat IgG2a M18/2 FITC Ms F
0.1 mg / €280.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

CD18 / ITGB2 Rat IgG2a M18/2 Purified Ms F, IP
0.25 mg / €390.00
  Acris Antibodies GmbH

CD18 / ITGB2 antibody

CD18 / ITGB2 Rat IgG2a M18/2 Purified Ms F, IP
0.1 mg / €230.00
  Acris Antibodies GmbH

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