CD181 / IL8RA antibody

Principal name

CD181 / IL8RA antibody

Alternative names for CD181 / IL8RA antibody

IL-8R A, IL-8 receptor type 1, CXCR-1, CXCR1, CMKAR1, CDw128a, IL8 receptor alpha, High affinity interleukin-8 receptor A

SwissProt ID

P25024 (Human), P70612 (Rat), Q810W6 (Mouse)

Gene ID

3577 (CXCR1), 227288 (Cxcr1)

Available reactivities

Hu (Human), Chimp (Chimpanzee), Ms (Mouse), Rb (Rabbit), Gorilla, Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Frozen Sections (C), Neutralisation (NEUT), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD181 / IL8RA antibody

Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CXCR1 (IL8RA, or type I IL8 receptor) and CXCR2 (IL8RB, or type II IL8 receptor) have been shown to share approximately 77% amino acid sequence identity. IL8 binds to both receptors with high affinity and induces rapid elevation of cytosolic Ca 2+ levels. Whereas CXCR1 is highly specific for IL8, CXCR2 has broad specificity and has been shown to bind with high-affinity to other ELR motif containing a chemokines including GROs alpha, beta and gamma and NAP-2, and ENA-78. In contrast, PF4 and IP10 (two chemokines that lack the ELR motif) have been shown to lack binding affinity for CXCR2. CXCR1 and CXCR2 are expressed by neutrophils but not B lymphocytes or T lymphocytes.

General readings

General / background references:Wells TN et al. Definition, function and pathophysiological significance of chemokine receptors. Trends Pharmacol Sci 19:376-80 (1998). PubMed PMID: 9786026
Moser B et al. Expression of transcripts for two interleukin 8 receptors in human phagocytes, lymphocytes and melanoma cells. Biochem J 294-pt1:285-92 (1993). PubMed PMID: 8363581
Murphy PM & Tiffany HL Cloning of complementary DNA encoding a functional human interleukin-8 receptor. Science 253:1280-3 (1991). PubMed PMID: 1891716

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD181 / IL8RA (N-term) antibody

Anti-CXCR1 antibody IHC of human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €345.00
  OriGene Technologies, Inc.

CD181 / IL8RA antibody

Anti-CXCR1 antibody IHC of human colon, plasma cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Hu, Mky
50 µg / €345.00
  OriGene Technologies, Inc.

CD181 / IL8RA antibody

Anti-CXCR1 antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 16 ug/ml. Rabbit IgG Purified Gorilla, Hu, Mky
50 µg / €345.00
  OriGene Technologies, Inc.

CD181 / IL8RA antibody

Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG2b kappa Isotype Control Purified (left) or 0.25 ug of Anti-Human CD181 (CXCR1) Purified (right) followed by F(ab')2 Anti-Mouse IgG PE .  Cells in the large scatter population were used for analysis. Mouse eBio8F1-1-4 (8F1-1-4) Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD181 / IL8RA antibody

Western blot analysis of CXCR1 in Jurkat cell lysate: 1. Anti-CXCR1 (extracellular) antibody, (1:200). 2. Anti- CXCR1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

CD181 / IL8RA antibody

TA334182 (0.3µg/ml) staining of Human Breast cancer lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD181 / IL8RA antibody

Isolated human neutrophils were incubated with 0.25 μg anti-human CXCR1 followed by staining with goat F (ab’)2 anti-mouse IgG FITC [shaded peak]. Specificity of signal was confirmed by staining the neutrophils with 0.25 μg mouse IgG1 isotype control followed by the secondary antibody [unshaded peak]. The data indicate that 100% of the isolated neutrophil population stains positive for this IL-8 receptor (M2 region). Mouse IgG1 501 Purified Hu C, E, F, ICC/IF, WB
0.5 mg / €1,010.00
  Acris Antibodies GmbH

CD181 / IL8RA antibody

Isolated human neutrophils were incubated with 0.25 μg anti-human CXCR1 followed by staining with goat F (ab’)2 anti-mouse IgG FITC [shaded peak]. Specificity of signal was confirmed by staining the neutrophils with 0.25 μg mouse IgG1 isotype control followed by the secondary antibody [unshaded peak]. The data indicate that 100% of the isolated neutrophil population stains positive for this IL-8 receptor (M2 region). Mouse 501 Purified Hu C, E, F, ICC/IF, WB
0.1 mg / €550.00
  Acris Antibodies GmbH

CD181 / IL8RA Control Peptide

CD181 / IL8RA
0.25 mg / €250.00
  Acris Antibodies GmbH

CD181 / IL8RA (N-term) antibody

CD181 / IL8RA Rabbit Aff - Purified Hu C, E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CD181 / IL8RA Control Peptide

CD181 / IL8RA
0.25 mg / €250.00
  Acris Antibodies GmbH

CD181 / IL8RA (C-term) antibody

CD181 / IL8RA Rabbit Aff - Purified Hu C, E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CD181 / IL8RA Control Peptide

CD181 / IL8RA
0.25 mg / €250.00
  Acris Antibodies GmbH

CD181 / IL8RA (N-term) antibody

CD181 / IL8RA Goat Aff - Purified Ms C, E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CD181 / IL8RA antibody

CD181 / IL8RA Mouse IgG1 501 Hu C, E, F, ICC/IF, WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

CD181 / IL8RA antibody

Western blot analysis of 15 µg of whole cell lysate from NIH/3T3 cells with IL8RA polyclonal antibody ( Cat # PAB0178 ) at 2 µg/ml dilution. Rabbit Hu, Ms WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CD181 / IL8RA antibody

Western blot of IL8RA polyclonal antibody ( Cat # PAB8941 ) with protein-coupled IL8RA. Antibody dilution at 1:500. Rabbit Hu C, IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

CD181 / IL8RA antibody

CD181 / IL8RA Rabbit IgG Aff - Purified Hu P, WB
0.1 mg / €290.00
  Boster Immunoleader

CD181 / IL8RA antibody

Western blot analysis of CD181 expression in HEK293T (A); NIH3T3 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CD181 / IL8RA antibody

Western blot analysis of CD181 expression in HEK293T (A); NIH3T3 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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