CD182 / IL8RB antibody

Principal name

CD182 / IL8RB antibody

Alternative names for CD182 / IL8RB antibody

CXCR2, CXCR-2, GRO/MGSA receptor, IL-8 receptor type 2, CDw128b, IL8 receptor beta

SwissProt ID

O97571 (Canfa), P25025 (Human), P35343 (Mouse), P35407 (Rat), Q28003 (Bovin)

Gene ID

3579 (CXCR2), 12765 (Cxcr2), 29385

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Mky (Monkey), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Neutralisation (NEUT), Frozen Sections (C), Flow Cytometry (F), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of CD182 / IL8RB antibody

Chemokines (CHEMOtactic cytoKINES) are an important subgroup of the inflammatory cytokine family. More than fifty chemokines are expressed in mammalian cells and are characterized by their relatively small size (~70-90 amino acids), by their conserved N-terminus and cysteine motifs. This group of proteins has been further categorized on the basis of the cysteine spacing in the motifs creating C, CC, CXC, and CX3C chemokine subfamilies. All fifty chemokines exert their effects through twenty different chemokine receptors, belonging to the superfamily of G-protein coupled receptors (GPCRs) suggesting a certain level of promiscuity among the different receptors. All chemokine receptors couple to the pertussis sensitive Gi protein leading to phospholipase C activation and adenylate cyclase inhibition. Chemokines were first identified by their ability to mediate leukocyte chemoattraction. Apart from regulating the migration of leukocytes, they seem to be major players during inflammation and immunity. Indeed, chemokines could also be further classified as being inflammatory as many chemokines are extensively upregulated in response to inflammation, or housekeeping important for the homeostasis of certain cell types. Inflammatory chemokines are responsible for recruiting immune cells to the inflamed region, and housekeeping chemokines, expressed in lymphoid or non-lymphoid tissues mediate the trafficking and targeting of cells. In general, chemokines and their receptors guide leukocytes to sites of infection/inflammation. However, cases of chronic inflammatory disease and tissue damage occur when there is excessive recruitment of leukocytes. They could also be involved in the pathogenesis of neurological diseases like multiple sclerosis and many inflammatory diseases like atherosclerosis and inflammatory bowel disease. Recently, chemokines and their receptors have been found to be involved in cancer metastasis, mely breast cancer. The chemokine sigling also seems to be important for the communication between neural cells and the immune system, especially in the context of infection. The CXCR2 receptor could mediate chemotaxis and degranulation of neutrophils along with CXCR1, via interleukin-8 (IL-8) a proinflammatory chemokine with the CXC motif. In addition, in a model of arterial injury, CXCR2 can improve endothelial recovery, and also seems to be important in recruiting endothelial progenitor cells to injured vessels. CXCR2 is activated by a plethora of ligands: GROa, b, g, neutrophil-activating peptide, granulocyte chemotactic protein-2 and keratinocyte derived chemokine (CXCL1). CXCR2 is expressed in different brain regions, in the lung and spleen and other regions.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD182 / IL8RB antibody

  Rabbit Polyclonal Anti-CXCR2 Antibody (N-Terminus)  
Anti-CXCR2 antibody IHC of human Adrenal, Pheochromocytoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €400.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

  Goat Anti-CD128 / Cxcr2 Antibody  
(0.3ug/ml) staining of Mouse Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

  Rabbit polyclonal IL-8R beta/CDw128 beta (Ser347) antibody(Phospho-specific)  
Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using IL-8R beta/CDw128 beta (Phospho-Ser347) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

  Mouse Anti-Human CD182 (CXCR2) Purified (100 ug)  
Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (left) or 0.25 ug of Anti-Human CD182 (CXCR2) Purified (right) followed by F(ab')2 Anti-Mouse IgG PE .  Cells in the large scatter population were used for analysis. Mouse eBio5E8-C7-F10 (5E8-C7-F10) Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

  Anti-CXCR2 Rabbit Polyclonal Antibody  
Western blot analysis of Human serum solution using TA321370(CXCR2 Antibody) at dilution 1/500 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

  Rabbit Polyclonal Anti-CXCR2 (extracellular)  
Western blot analysis of Jurkat (lanes 1 and 4), SH-SY5Y (lanes 2 and 5) and THP-1 (lanes 3 and 6) lysates: 1-3. Anti-CXCR2 (extracellular) antibody, (1:200). 4-6. Anti-CXCR2 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

CD182 / IL8RB (N-term) antibody

  Rabbit Polyclonal Anti-CXCR2 Antibody (N-Terminus)  
Anti-CXCR2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €400.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

  Rabbit Polyclonal Anti-IL8RB Antibody  
Human cystic fibrosis epitheial Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 6D499 Purified Hu F, P
50 µg / €530.00
  Acris Antibodies GmbH

CD182 / IL8RB antibody

Immunohistochemical analysis of paraffin embedded Human lung cancer sections using CXCR2 antibody Rabbit IgG Aff - Purified Hu IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CD182 / IL8RB antibody

CD182 / IL8RB Mouse IgG1 HC2 PE Hu F
100 Tests / €470.00
  Acris Antibodies GmbH

CD182 / IL8RB antibody

CD182 / IL8RB Mouse IgG1 HC2 PE Hu F
25 Tests / €210.00
  Acris Antibodies GmbH

CD182 / IL8RB pSer347 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

CD182 / IL8RB pSer347 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

CD182 / IL8RB antibody

Immunofluorescence analysis of HepG2 cells, using IL-8R beta/CDw128 beta (Ab-347) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF
50 µg / €230.00
  Assay Biotech

CD182 / IL8RB antibody

Immunofluorescence analysis of HepG2 cells, using IL-8R beta/CDw128 beta (Ab-347) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF
0.1 mg / €330.00
  Assay Biotech

CD182 / IL8RB antibody

CXCR2 staining of human lymph nodes. Cells were stained using ABC-HRP + AEC (red) and Haematoxylin (blue) counterstain. It is recommended to employ an avidin-biotin blocking step and quench endogenous peroxidase. Mouse IgG2a Purified Hu C, F, NEUT
0.1 mg / €410.00
  Neuromics Antibodies

CD182 / IL8RB antibody

Figure 1. Mouse MIP-2 chemoattracts BaF/3 cells that have been transfected with mCXCR2. The number of cells that have migrated through to the lower chamber are quantitated using Resazurin fluorescence staining. The ED50 for this effect is typically 0.1 - 0.5 ng/mL.
Figure 2. To measure the ability of the antibody to block rmMIP-2 induced chemotaxis of mCXCR2 transfected BaF/3 cells, rmMIP-2 at 2 ng/mL was added to the lower compartment of a 96-well chemotaxis chamber (NeuroProbe, Cabin John, MD). The chemotaxis chamber was then assembled using a PVP-free polycarbonate filter (5 micron pore size). Serial dilutions of the antibody (at the concentrations indicated) and 0.2 x 106 cells/well were added to the top wells of the chamber. After incubation for 3 hours at 37° C in a 5% CO2 humidified incubator, the chamber was disassembled and the cells that migrated through to the lower chamber were transferred to a working plate and quantitated using Resazurin Fluorescence. As shown in Figure 2, the ND50 for this lot of antibody is approximately 15 - 50 µg/mL. Mouse IgG2a Purified Ms F, NEUT
0.1 mg / €410.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

CXCR2 - human gene knockout kit via CRISPR

CXCR2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CXCR2 - human gene knockout kit via CRISPR

CXCR2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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