CD182 / IL8RB antibody

Principal name

CD182 / IL8RB antibody

Alternative names for CD182 / IL8RB antibody

CXCR2, CXCR-2, GRO/MGSA receptor, IL-8 receptor type 2, CDw128b, IL8 receptor beta

SwissProt ID

O97571 (Canfa), P25025 (Human), P35343 (Mouse), P35407 (Rat), Q28003 (Bovin)

Gene ID

3579 (CXCR2), 12765 (Cxcr2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Paraffin Sections (P), Neutralisation (NEUT), Frozen Sections (C), Flow Cytometry (F), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of CD182 / IL8RB antibody

Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CXCR1 (IL8RA, or type I IL8 receptor) and CXCR2 (IL8RB, or type II IL8 receptor) have been shown to share approximately 77% amino acid sequence identity. IL8 binds to both receptors with high affinity and induces rapid elevation of cytosolic Ca 2+ levels. Whereas CXCR1 is highly specific for IL8, CXCR2 has broad specificity and has been shown to bind with high-affinity to other ELR motif containing a chemokines including GRO alpha, GRO beta, GRO gamma, NAP2 and ENA78. In contrast, PF4 and IP10 (two chemokines that lack the ELR motif) have been shown to lack binding affinity for CXCR2. CXCR1 and CXCR2 are expressed by neutrophils but not B lymphocytes or T lymphocytes.

General readings

Wells TN et al. Definition, function and pathophysiological significance of chemokine receptors. Trends Pharmacol Sci 19:376-80 (1998). PubMed PMID: 9786026
Lee J et al. Characterization of two high affinity human interleukin-8 receptors. J Biol Chem 267:16283-7 (1992). PubMed PMID: 1379593
Murphy PM & Tiffany HL Cloning of complementary DNA encoding a functional human interleukin-8 receptor. Science 253:1280-3 (1991). PubMed PMID: 1891716

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD182 / IL8RB antibody

Anti-CXCR2 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

Anti-CXCR2 antibody IHC of human Adrenal, Pheochromocytoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

(0.3ug/ml) staining of Mouse Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (left) or 0.25 ug of Anti-Human CD182 (CXCR2) Purified (right) followed by F(ab')2 Anti-Mouse IgG PE .  Cells in the large scatter population were used for analysis. Mouse eBio5E8-C7-F10 (5E8-C7-F10) Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

Western blot analysis of Human serum solution using TA321370(CXCR2 Antibody) at dilution 1/500 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD182 / IL8RB antibody

Western blot analysis of Jurkat (lanes 1 and 4), SH-SY5Y (lanes 2 and 5) and THP-1 (lanes 3 and 6) lysates: 1-3. Anti-CXCR2 (extracellular) antibody, (1:200). 4-6. Anti-CXCR2 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

CD182 / IL8RB (N-term) antibody

Anti-CXCR2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

CD182 / IL8RB (N-term) antibody

Isolated human neutrophils were incubated with 1.0 μg anti-human CXCR2 followed by staining with goat F (ab’)2 anti-mouse IgG FITC [shaded peak]. Specificity of signal was confirmed by staining the neutrophils with 1.0 μg mouse IgG1 isotype control followed by the secondary antibody [unshaded peak]. The data indicate that approximately 98% of the isolated neutrophil population stains positive for this IL-8 receptor (M2 region). Mouse IgG1 19 Purified Hu C, E, F, FN, WB
0.5 mg / €1,010.00
  Acris Antibodies GmbH

CD182 / IL8RB (N-term) antibody

Figure 1. Isolated human neutrophils were incubated with 1.0 μg anti-Human CXCR2 antibody followed by staining with goat F(ab`)2 anti-mouse IgG FITC [shaded peak]. Specificity of signal was confirmed by staining the neutrophils with 1.0 μg mouse IgG1 isotype control followed by the secondary antibody [unshaded peak]. The data indicate that approximately 98% of the isolated neutrophil population stains positive for this IL-8 receptor (M2 region). Mouse IgG1 19 Purified Hu C, E, F, FN, ICC/IF, P, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

CD182 / IL8RB antibody

Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 6D499 Purified Hu F, P
50 µg / €470.00
  Acris Antibodies GmbH

CD182 / IL8RB Control Peptide

CD182 / IL8RB
0.25 mg / €250.00
  Acris Antibodies GmbH

CD182 / IL8RB antibody

Western blot of Chemokine receptor with AP09955PU-N (1:500). MWM on Right. Rabbit Aff - Purified Hu, Mky C, E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CD182 / IL8RB Control Peptide

CD182 / IL8RB
0.25 mg / €420.00
  Acris Antibodies GmbH

CD182 / IL8RB antibody

CD182 / IL8RB Rabbit Aff - Purified Hu, Ms, Rt C, E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CD182 / IL8RB antibody

Immunohistochemical analysis of paraffin embedded Human lung cancer sections using CXCR2 antibody Rabbit IgG Aff - Purified Hu IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CD182 / IL8RB antibody

CD182 / IL8RB Mouse IgG1 HC2 PE Hu F
100 Tests / €470.00
  Acris Antibodies GmbH

CD182 / IL8RB antibody

CD182 / IL8RB Mouse IgG1 HC2 PE Hu F
25 Tests / €210.00
  Acris Antibodies GmbH

CD182 / IL8RB antibody

Western Blot analysis of IL8RB expression in transfected 293T cell line (H00003579-T01) by IL8RB MaxPab polyclonal antibody.

Lane 1: IL8RB transfected lysate(39.6 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD182 / IL8RB antibody

CD182 / IL8RB Mouse IgG2b 3M9 Hu E
50 µg / €370.00
  Abnova Taiwan Corp.

CD182 / IL8RB antibody

CD182 / IL8RB Mouse IgG1 19 Hu C, E, F, ICC/IF, WB
0.1 mg / €480.00
  Abnova Taiwan Corp.

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