CD185 / CXCR5 antibody

Principal name

CD185 / CXCR5 antibody

Alternative names for CD185 / CXCR5 antibody

CXC-R5, CXCR-5, BLR1, MDR15, C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, Monocyte-derived receptor 15

SwissProt ID

P32302 (Human), P34997 (Rat), Q04683 (Mouse)

Gene ID

643 (CXCR5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Hst (Hamster), Por (Porcine), Rb (Rabbit), Bear, Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C), Neutralisation (NEUT)

Background of CD185 / CXCR5 antibody

Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. Naive B cells express CXCR5, which has been shown by gene targeting experiments to be essential for the formation of certain follicles and germinal centers. The human CXCR5 ligand, B-cell attracting chemokine (BCA1, BLC), and its mouse homologue, B lymphocyte chemoattractant (BLC), 4 are produced by cells in follicles and may play a role in organizing their structure by attracting naive B cells as well as activated Th (T helper) cells.

General readings

Wells TN et al. Definition, function and pathophysiological significance of chemokine receptors. Trends Pharmacol Sci 19:376-80 (1998). PubMed PMID: 9786026
Gunn MD et al. A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1. Nature 391:799-803 (1998). PubMed PMID: 9486651
Forster R et al. A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen. Cell 87:1037-47 (1996). PubMed PMID: 8978608

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD185 / CXCR5 (3rd Cytopl. Dom.) antibody

Anti-CXCR5 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Aff - Purified Bear, Bov, Can, Hst, Hu, Ms, Por, Rb, Rt P
50 µg / €379.00
  OriGene Technologies, Inc.

CD185 / CXCR5 (C-term) antibody

Anti-CXCR5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Can, Hst, Hu, Mky, Ms, Por, Rt P
50 µg / €379.00
  OriGene Technologies, Inc.

CD185 / CXCR5 antibody

Predicted cell location: Cell membrane, Cytoplasm. Positive control: Human rectal cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human rectal cancer tissue using CXCR5 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD185 / CXCR5 Control Peptide

CD185 / CXCR5 Purified
50 µg / €190.00
  Acris Antibodies GmbH

CD185 / CXCR5 antibody

Western blot analysis of CXCR5 expression with Jurkat cell lysate Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CD185 / CXCR5 antibody

Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) using CD185/CXCR5 antibody Rabbit Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD185 / CXCR5 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CXCR5 and GNAI2. HeLa cells were stained with anti-CXCR5 rabbit purified polyclonal 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CD185 / CXCR5 antibody

Detection limit for recombinant GST tagged BLR1 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2a 2C1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

CD185 / CXCR5 antibody

Western blot analysis of 20 µg of total cell lysate from Daudi cells with BLR1 polyclonal antibody ( Cat # PAB0175 ) at 2 µg/ml dilution. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CD185 / CXCR5 antibody

Western blot analysis of CXCR5 expression in Jurkat cell lysate. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CD185 / CXCR5 antibody

CD185 / CXCR5 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

CD185 / CXCR5 antibody

CD185 / CXCR5 Mouse IgG2b 51505,111 Purified Hu F
0.1 mg / €340.00
  Novus Biologicals Inc.

CD185 / CXCR5 antibody

Spleen Rabbit Aff - Purified Hu E, P
50 µl / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD185 / CXCR5

CD185 / CXCR5 Human
10 µg / €540.00
  Abnova Taiwan Corp.

CD185 / CXCR5

CD185 / CXCR5 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

15 Item(s)

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