CD186 / CXCR6 antibody

Principal name

CD186 / CXCR6 antibody

Alternative names for CD186 / CXCR6 antibody

CXC-R6, CXCR-6, BONZO, STRL33, TYMSTR, CDw186, G-protein coupled receptor bonzo, G-protein coupled receptor STRL33

SwissProt ID

O00574 (Human), Q9EQ16, Q9TV16

Gene ID

10663 (CXCR6), 80901 (Cxcr6)

Available reactivities

Hu (Human), Chimp (Chimpanzee), Can (Canine), Ms (Mouse), Rt (Rat), Bt (Bat), Bov (Bovine), Eq (Equine), Mky (Monkey), Por (Porcine), Bear, Gorilla

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Enzyme Immunoassay (E)

Background of CD186 / CXCR6 antibody

Chemokine (Chemoattractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells. Chemokine receptors (CXCR) belong to the super-family of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and proliferation and migration of immature neurons, glia and their precursors (1). Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases. Several subtypes of CXCR have been characterized (Table1).
Activation of naïve T cells by specific antigen and cytokines, up-regulate cell adhesion molecules (CAM) as well as chemokine receptors on their surface, which directs them to migrate towards the inflamed tissues. CXCR6, originally identified as a fusion cofactor for human immunodeficiency virus (HIV)-1 and simian immunodeficiency virus (SIV) and designated Bonzo, STRL33 and TYMSTR, is a member of the seven-transmembrane domain GPCR family. In humans, CXCR6 is preferentially expressed by subsets of memory/effector T cells, natural killer (NK) cells and NK T cells, and is also found on plasma cells. CXCR6 was present on 4–6% of CD4 and CD8 T cells in blood, normal lymph nodes (LNs) and the spleen, primarily on memory T cells (2). The only known ligand for CXCR6 is CXCL16 which is expressed as membrane bound form on antigen presenting cells such as dentritic cell, B cells and mono nuclear phagocytes, endothelial and smooth muscle cells. CXCR6 is also described as scavenger receptor for Phosphotidyl serine and oxidized lipoprotein on macrophages (3) however, this bifunctional role is not fully understood.
The CXCR6 receptor protein is approximately 42-45kDa (342 amino acids) protein which has 7 trans-membrane domains, characteristic of G-protein coupled receptors. The all CXCR sequence analyses suggest the presence of 7 trans-membrane domains, a characteristic feature of GPCR. The protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins. The short carboxy terminal is intracellular and has putative post-translational sites.

General readings

1. Lin S, Sun L, Hu J, Wan S, Zhao R, Yuan S, Zhang L,
Chemokine C-X-C motif receptor 6 contributes to cell migration during hypoxia.
Cancer letters 2009 Jun 279 (1): 108-17.
PMID: 19231068

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD186 / CXCR6 (2nd cytpopl. dom.) antibody

  CXCR6 Rabbit Polyclonal (Cytoplasmic Domain) Antibody  
Anti-CXCR6 antibody IHC of human tonsil, interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Bt, Can, Eq, Hu, Mky, Por P
50 µg / €400.00
  OriGene Technologies, Inc.

CD186 / CXCR6 antibody

  CXCR6 Rabbit Polyclonal (C-Terminus) Antibody  
Anti-CXCR6 antibody IHC of human tonsil, immunoblasts. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Gorilla, Hu, Mky
50 µg / €400.00
  OriGene Technologies, Inc.

CD186 / CXCR6 (C-term) antibody

  Rabbit Polyclonal Bonzo Antibody  
Western blot analysis of Bonzo in SW1353 total cells lysate with Bonzo antibody at 1:1000 dilution. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD186 / CXCR6 antibody

  Rabbit Polyclonal Bonzo Antibody  
Western blot analysis of Bonzo in human spleen tissue lysate in the absence (lane A) or presence (lane B) of peptide with Bonzo antibody (NT2) at 1:500 dilution.  Below: Immunohistochemistry of Bonzo in human spleen cells with Bonzo antibody at 20 µg/ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD186 / CXCR6 antibody

  Goat Anti-CXCR6 Antibody  
(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD186 / CXCR6 antibody

  CXCR6 Rabbit Polyclonal (C-Terminus) Antibody  
Anti-CXCR6 antibody IHC of human tonsil, interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Eq, Gorilla, Hu, Mky, Por
50 µg / €400.00
  OriGene Technologies, Inc.

CD186 / CXCR6 antibody

  Rabbit Polyclonal Anti-CXCR6 Antibody  
WB Suggested Anti-CXCR6 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD186 / CXCR6 antibody

  Rabbit Polyclonal Anti-CXCR6 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (CXCR6 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD186 / CXCR6 antibody

WB analysis CDw186 in SW1353 total cells lysate with this antibody Rabbit IgG Purified Hu WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD186 / CXCR6 antibody

Thymus: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD186 / CXCR6 (11-25) antibody

Western blot analysis of CDw186 (also known as CXCR6/Bonzo) in human spleen tissue lysate in the absence (lane A) or presence (lane B) of peptide with SP1286P Rabbit Aff - Purified Hu WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD186 / CXCR6 (319-338) antibody

Figure 1. Western blot analysis using SP6322PX antibody at 0.5 µg/ml on SW1353 cell lysates. Rabbit IgG Purified Hu WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CD186 / CXCR6 (11-25) antibody

Figure 1. Western blot analysis using SP6323PX antibody at 1 µg/ml on human spleen tissue lysates. Rabbit IgG Purified Hu WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

CXCR6 - human gene knockout kit via CRISPR

CXCR6 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

CXCR6 - human gene knockout kit via CRISPR

CXCR6 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

CXCR6 - human gene knockout kit via CRISPR

CXCR6 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

CXCR6 - human gene knockout kit via CRISPR

CXCR6 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cxcr6 - mouse gene knockout kit via CRISPR

Cxcr6 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Cxcr6 - mouse gene knockout kit via CRISPR

Cxcr6 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Cxcr6 - mouse gene knockout kit via CRISPR

Cxcr6 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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