CD192 / CCR2 antibody

Principal name

CD192 / CCR2 antibody

Alternative names for CD192 / CCR2 antibody

C-C CKR-2, CC-CKR-2, CCR-2, CMKBR2, C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP1-Receptor

SwissProt ID

O55193 (Rat), P41597 (Human), P51683 (Mouse)

Gene ID

729230 (CCR2), 12772 (Ccr2), 1231, 60463

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Paraffin Sections (P), Flow Cytometry (F), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of CD192 / CCR2 antibody

Chemokine receptors represent a subfamily of ~20 GPCRs that were originally identified by their roles in immune cell trafficking. Macrophage inflammatory protein-1 alpha (MIP-1 alpha) and RANTES, members of the beta chemokine family of leukocyte chemo-attractants, bind to a common seven-transmembrane-domain human receptor. Chemokine (Chemo-attractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells. Chemokine receptors (CCR) belong to the superfamily of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and directing the proliferation and migration of immature neurons, glia and their precursors (1). Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases. Induction of chemokines and infiltration of chemokine receptor-bearing cells has also been shown in a variety of animal models of renal diseases, as well as in human diseases and allograft rejection (2) Monocyte activation protein 1 (MACP1) or CCL2 is the ligand for CCR2, upon activation CCR2 mediate robust release of Ca+2 and monocytic infilteration of tissues in atherosclerosis and other inflammatory diseases. The CCR2 receptors are most closely related to MCP1/CCR1/RANTES receptors.CCR2 receptor are also implicated in several neuropathological syndromes including neuropathic pain (3). CCR2 and CXCR3 receptors also mediate leukocyte adhesion and migration in to acute kidney injury caused by ischemia-reperfusion. CCR2 is a multi-pass membrane protein, containing 7TMDs with apparent MW of 46kDa (373 aa) expressed on many different blood cells. The CCR2 protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins. The short carboxy terminal is intracellular and has putative post-translational sites. The Anti-CCR2-selective antibodies were generated against conserved but unique sequences from N-terminal region of human CCR2 receptor proteins. The peptide sequence resides between aa 30-60 of CCR2 and are unique to CCR2 protein. Antibodies generated against this peptide are specific for CCR2, these antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. The affinity purified antibodies are also conjugated to FITC and biotin for direct applications in IHC and cell sorting experiments.

General readings

General / background references:Wells TN et al. Definition, function and pathophysiological significance of chemokine receptors. Trends Pharmacol Sci 19:376-80 (1998). PubMed PMID: 9786026
Charo IF et al. Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails. Proc Natl Acad Sci U S A 91:2752-6 (1994). PubMed PMID: 8146186

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD192 / CCR2 antibody

Western Blot: CCR2 Antibody [TA336782] - WB analysis of CCR2 in rat lysate. Image courtesy of product review submitted by Rasha Elbaz. Rabbit Purified Hu, Rt C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD192 / CCR2 antibody

Western Blot: CCR2 Antibody (3G7) [TA337219] - Western blot analysis of CCR2 [3G7] in Molt4 lysate. Mouse IgG2b 3G7 Purified Hu C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD192 / CCR2 antibody

Western blot analysis of CCR2 expression in lysate from rat kidney (Lane 1), 3T3 cells (Lane 2) and Jurkat cells (Lane 3). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD192 / CCR2 antibody

Immunocytochemistry/Immunofluorescence: CCR2 Antibody [TA336783] - Analysis of CCR2 in NIH/3T3 cells Rabbit IgG Purified Ms F, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD192 / CCR2 antibody

Western Blot: CCR2 Antibody (2D8) [TA337218] - Western blot analysis of CCR2 [2D8] in Molt4 lysate Mouse IgG2b 2D8 Purified Hu C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD192 / CCR2 (N-term, extracel. dom.) antibody

Anti-CCR2 antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Mky, Prim P
50 µg / €379.00
  OriGene Technologies, Inc.

CD192 / CCR2 (2nd Extracell. Dom) antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Prim P
50 µg / €379.00
  OriGene Technologies, Inc.

CD192 / CCR2 antibody

WB Suggested Anti-CCR2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 721_B cell lysate Rabbit IgG Purified Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD192 / CCR2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCR2 (RC221283, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCR2. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD192 / CCR2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCR2 (RC221283, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCR2. Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD192 / CCR2 antibody

Immunohistochemistry on paraffin sections: Staining with AM26722PU-N on Human tonsil. Mouse IgG2a 7A7 Purified Hu P
0.2 mg / €420.00
  Acris Antibodies GmbH

CD192 / CCR2 antibody

CD192 / CCR2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

CD192 / CCR2 antibody

CCR2 monoclonal antibody (M01A), clone 4D12 Western Blot analysis of CCR2 expression in HepG2 ( Cat # L019V1 ). Mouse IgM 4D12 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

CD192 / CCR2 antibody

CD192 / CCR2 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

CD192 / CCR2 antibody

Western blot analysis of Ccr2 expression in lysate from rat kidney ( Lane 1 ) , NIH/3T3 cells ( Lane 2 ) and Jurkat cells ( Lane 3 ) . Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CD192 / CCR2 antibody

Human 721_B; WB Suggested Anti-CCR2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate; CCR2 antibody - middle region (ARP58409_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

CD192 / CCR2 antibody

CD192 / CCR2 Rabbit IgG Purified Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

CD192 / CCR2 antibody

ELISA: CCR2 Antibody [19450002] Rabbit Aff - Purified Hu E, WB
50 µg / €440.00
  Novus Biologicals Inc.

CD192 / CCR2 antibody

Immunohistochemistry: CCR 2 Antibody [NB100-701] - Immunohistochemistry of NB100-701 antibody to mouse CCR2 on paraffin section of mouse spleen Goat Aff - Purified Ms F, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

CD192 / CCR2 antibody

Immunohistochemistry: CCR 2 Antibody [NB100-718] - Immunohistochemistry on paraffin section of human spleen Goat Aff - Purified Hu E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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