CD194 / CCR4 antibody

Principal name

CD194 / CCR4 antibody

Alternative names for CD194 / CCR4 antibody

C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, CMKBR4, K5-5

SwissProt ID

P51679 (Human), P51680 (Mouse), Q8MJW8 (Canfa)

Gene ID

1233 (CCR4), 4887 (NPY2R), 12773

Available reactivities

Hu (Human), Bov (Bovine), Mky (Monkey), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Gorilla, Bt (Bat), Hst (Hamster), Op (Opossum), Prim (Primate), Rb (Rabbit), Bear

Available hosts

Rabbit, Goat, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of CD194 / CCR4 antibody

Chemokine (Chemoattractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells. Chemokine receptors (CCR) belong to the superfamily of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and directing the proliferation and migration of immature neurons, glia and their precursors (1). Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases. Induction of chemokines and infiltration of chemokine receptor-bearing cells has also been shown in a variety of animal models of renal diseases, as well as in human diseases and allograft rejection (2).
Activation of naïve T cells by specific antigen and cytokines, up-regulate cell adhesion molecules (CAM) as well as chemokine receptors on their surface, which directs them to migrate towards the inflamed tissues. Several subtypes of CCR have been characterized (Table1). The CCR4 recrptors are activated by its ligand CCL17/TARC. CCR4 has been implicated Not complex function parallel to or down stream of the bur1/2 that facilitate trimethylation of Histone H3 and regulate the transcription activity (3). CCR4 is also involved in the chodrogenesis of mesenchymal stem cells, this process is influenced by many molecules including CXCL1, FIT3L, CCR3 and CCR4. The induction of CCR4 positive cell in duodenuim affects the food allergy, in skin-tropic condition, in allergic bronchopulmonary aspergillosis (ABPA) is a rare variant of severe asthma resulting from hypersensitivity to Aspergillus fumigatus. The expression of CCR4 is decreases in several autoimmune diseases such as diabetes type1 and rheumatoid arthritis.
The CCR4 receptor protein is approximately 45 kDa (360 amino acids) protein which has 7 transmembrane domains, characteristic of G-protein coupled receptors. The CCR4 receptors are activated by Thymus and activation-regulated chemokine (TARC) that is expressed constitutively in thymus and transiently in stimulated peripheral blood mononuclear cells. The protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins. The short carboxy terminal is intracellular and has putative post-translational sites.

General readings

This product has been used in:Hoogewerf A et al. Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4. Biochem Biophys Res Commun 218:337-43 (1996). PubMed PMID: 8573157
2. Jakubzick, C., et al. Roel of CCR4 ligands, CCL17 and CCL22, during Schitosome manosi egg-induced pulmonaey granuloma formation in mice. Am. J. Pathol. 165(4): 1211-1221, 2004.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD194 / CCR4 antibody

Immunostaining for CCR-4 in anaplastic large-cell lymphoma, showing membrane and granular cytoplasmic reactivity in all tumor cells while admixed small lymphocytes are negative. Mouse IgG1 Purified Hu ICC/IF
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD194 / CCR4 antibody

Anti-CCR4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Gorilla, Hu, Mky, Por
50 µg / €345.00
  OriGene Technologies, Inc.

CD194 / CCR4 (C-term) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Bov, Bt, Can, Elephant, Hst, Hu, Mky, Op, Prim, Rb P
50 µg / €345.00
  OriGene Technologies, Inc.

CD194 / CCR4 antibody

Anti-CCR4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu E, P
50 µg / €345.00
  OriGene Technologies, Inc.

CD194 / CCR4 antibody

Anti-CCR4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €345.00
  OriGene Technologies, Inc.

CD194 / CCR4 antibody

WB Suggested Anti-CCR4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysateCCR4 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD194 / CCR4 Control Peptide

CD194 / CCR4 Hu
0.25 mg / €250.00
  Acris Antibodies GmbH

CD194 / CCR4 antibody

CD194 / CCR4 Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt C, E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CD194 / CCR4 (Extracell. Dom.) antibody

Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Prim, Rb E, F, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD194 / CCR4 antibody

AP26357PU-N: Western blot analysis of CCR4 using Jurkat cell lysates. Rabbit Aff - Purified Can, Hu, Ms WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CD194 / CCR4 antibody

Figure 2: Spectral Confocal Microscopy of CHO cells using KH-4F5 antibody (Cat.#DM1232). CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue). Mouse IgG2a KH-4F5 Purified Hu E, F, ICC/IF
0.1 mg / €330.00
  Acris Antibodies GmbH

CD194 / CCR4 antibody

Western blot analysis of CCR4 expression in Jurkat cell lysates. Rabbit Hu, Ms WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

CD194 / CCR4 antibody

Immunofluorescence analysis of HuvEc cells, using CCR4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

CD194 / CCR4 antibody

Immunofluorescence analysis of HuvEc cells, using CCR4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

CD194 / CCR4 antibody

Human 721_B; WB Suggested Anti-CCR4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. CCR4 is supported by BioGPS gene expression data to be expressed in 721_B.; CCR4 antibody - middle region (AVARP07017_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

CD194 / CCR4 antibody

CD194 / CCR4 Rabbit IgG Aff - Purified Hu P, WB
0.1 mg / €290.00
  Boster Immunoleader

CD194 / CCR4 antibody

CD194 / CCR4 Rabbit IgG Aff - Purified Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

CD194 / CCR4 antibody

Western blot analysis of CCR4 expression in HL60 (A); MCF7 (B) whole cell lysates. Rabbit Aff - Purified Bov, Hu WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

CD194 / CCR4 antibody

Western blot analysis of CCR4 expression in HL60 (A); MCF7 (B) whole cell lysates. Rabbit Aff - Purified Bov, Hu WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

CD194 / CCR4 antibody

Western blot analysis of CCR4 expression in HL60 (A); MCF7 (B) whole cell lysates. Rabbit Aff - Purified Bov, Hu WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

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