CD195 / CCR5 antibody

Principal name

CD195 / CCR5 antibody

Alternative names for CD195 / CCR5 antibody

C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CMKBR5, CHEMR13, HIV-1 fusion coreceptor

SwissProt ID

O08556 (Rat), P51681 (Human), P51682 (Mouse)

Gene ID

12774 (Ccr5), 1234 (CCR5)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Macaque, Prim (Primate)

Available hosts

Mouse, Rabbit, Goat, Rat, Hamster

Available applications

Frozen Sections (C), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of CD195 / CCR5 antibody

Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The alpha and beta families, with four cysteine residues, are the largest and best characterized. In the alpha family, one amino acid separates the first two cysteine residues (CXC); in the beta family the two cysteine residues (CC) are adjacent to each other. The alpha chemokines that contain the N-terminal Glu-Leu-Arg amino acid sequence (ELR-motif) are chemotactic for neutrophils (such as IL8), while those that do not, act on lymphocytes (such as IP10 and MIG). Examples of chemokines under the beta family category are MCP1-5 and RANTES. The chemokine lymphotactin belongs to the gamma family, with only two cysteines (C), and the recently described fractalkine or neurotactin is a member of the delta family and has the first two cysteine residues separated by three amino-acids (CXXXC). Chemokines bind to specific G protein-coupled cell surface receptors on target cells. Five CXC receptors (CXCR1-5), nine CC receptors (CCR1-9) and one CXXXC receptor (CX3CR1) have been cloned to date. Expression of chemokine receptors can be restricted to some cell types (CXCR1 is expressed in neutrophils) while others (such as CCR2) are expressed in a wide variety of cells. Receptor expression has also been found to be constitutive (including down regulation), inducible or restricted to a cell state of activation. In addition, some chemokine receptors are also expressed in non-hematopoietic cells, such as nerve, endothelial and epithelial cells. This suggests that chemokines have other roles besides leucocyte chemotaxis. CX3CR1, for example, is highly expressed in adult brain. Chemokine receptors are linked to phospholipases through the Gi class of G proteins (inhibition by pertussis toxin). Receptor activation leads to a cascade of cellular events including generation of inositol triphosphate, calcium release and activation of protein kinase C. Chemokine receptors also activate small GTP-binding proteins of the Ras and Rho families, the latter being involved in cell motility events. In addition, chemokines bind to non-signaling molecules such as the Duffy antigen receptor for chemokines (DARC) which may act to remove chemokines from the circulation, and heparan sulfates proteoglycans which may serve to establish an ECM concentration gradient. CCR5 has 48 - 75% amino acid sequence identity to earlier identified CC receptors. It is expressed in primary adherent monocytes, but not in neutrophils or eosinophils. CCR5 mediates the activities of MIP1a, MIP1b and RANTES. Recently, it has also been shown to be a co-receptor on CD4+ target cells for infection with primary, monocyte-tropic HIV1 viruses.

General readings

General / background references:Wells TN et al. Definition, function and pathophysiological significance of chemokine receptors. Trends Pharmacol Sci 19:376-80 (1998). PubMed PMID: 9786026
Combadiere C et al. Cloning and functional expression of CC CKR5, a human monocyte CC chemokine receptor selective for MIP-1(alpha), MIP-1(beta), and RANTES. J Leukoc Biol 60:147-52 (1996). PubMed PMID: 8699119
Combadiere C et al. Cloning and functional expression of a human eosinophil CC chemokine receptor. J Biol Chem 270:16491-4 (1995). PubMed PMID: 7622448

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD195 / CCR5 antibody

Western blot analysis of CCR5 in THP-1 whole cell lysate with CCR5 antibody at 1:1000 dilution. Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD195 / CCR5 antibody

Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD195 (CCR5) Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE .  Cells in the lymphocyte gate were used for analysis. Mouse eBioT21/8 (T21/8) Purified Hu F
25 µg / €120.00
  OriGene Technologies, Inc.

CD195 / CCR5 antibody

CD195 / CCR5 Hamster HM-CCR5 (7A4) Purified Ms F
0.1 mg / €150.00
  OriGene Technologies, Inc.

CD195 / CCR5 antibody

CD195 / CCR5 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD195 / CCR5 antibody

Western Blot: CCR5 Antibody [TA336316] - WB Image of CCR5 in human liver lysate. Rabbit Purified Hu, Prim E, F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD195 / CCR5 antibody

Western Blot: CCR5 Antibody [TA337215] - WB detection of CCR5 partial recombinant protein by using CCR5 antibody at a concentration of 0.5 ug/ml Rabbit Purified Hu C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD195 / CCR5 non pSer337/349 antibody

CD195 / CCR5 Mouse IgG1 R-C10 Purified Hu E, ICC/IF, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

CD195 / CCR5 pSer337 antibody

CD195 / CCR5 Mouse IgG2b V14/2 Purified Hu E, ICC/IF, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

CD195 / CCR5 pSer349 antibody

CD195 / CCR5 Mouse IgG1 E11/19 Purified Hu E, ICC/IF, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

CD195 / CCR5 antibody

Formalin-Fixed, Paraffin-Embedded Human Tonsil stained with CCR5 Antibody Cat.-No AM20421PU-N at 20 µg/ml after heat-induced antigen retrieval. Mouse IgG1 T21/8 Aff - Purified Hu E, F, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD195 / CCR5 antibody

Flow cytometric analysis of CD195/CCR5 expression on peripheral blood lymphocytes. Open histogram indicates the reaction of Isotypic control to the cells. Shaded histogram indicates the reaction of AM26474AF-N to the cells. Rat IgG1 T227 Azide Free Hu F
0.1 mg / €360.00
  Acris Antibodies GmbH

CD195 / CCR5 antibody

Flow cytometric analysis of CD195 expression on human peripheral blood lymphocytes. Shaded histogram indicates the reaction of isotypic control to the cells. Open histogram indicates the reaction of AM26474FC-N to the cells. Rat IgG1 T227 FITC Hu F
1 ml / €350.00
  Acris Antibodies GmbH

CD195 / CCR5 antibody

Western blot analysis using anti-CCR5 (NT) antibody at 0.5 µg/ml on THP-1 whole cell lysates. Rabbit IgG Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

CD195 / CCR5 (6-20) antibody

Staining of human lymph node tissue with Rabbit anti Human CCR5 (NT) at 20 µg/ml. Formalin fixed paraffin processed tissue. (AP05821PU-N) Rabbit IgG Purified Hu IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD195 / CCR5 antibody

Western blot (WB) analysis of CCR5 antibody (Cat.-No.: AP06378PU-N) in extracts from COS7 cells. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD195 / CCR5 (3-30) antibody

Figure 1. Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded Human Spleen tissue using AP08778PU-N at 5µg/ml. Goat IgG Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD195 / CCR5 (N-term) antibody

Immunohistochemical analysis of paraffin embedded rectal carcinoma sections, staining CCR5 in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CD195 / CCR5 pSer349 antibody

Western blot analysis of extracts from A549 cells (Lane 2), using CCR5 (Phospho-Ser349) Antibody AP55693PU-N. The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu WB
0.1 ml / €340.00
  Acris Antibodies GmbH

CD195 / CCR5 pSer349 antibody

Western blot analysis of extracts from A549 cells (Lane 2), using CCR5 (Phospho-Ser349) Antibody AP55693PU-N. The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu WB
50 µl / €270.00
  Acris Antibodies GmbH

CD195 / CCR5 antibody

Figure 2: SDS-PAGE analysis of purified NP-6G4 monoclonal antibody. 
Lane 1: Molecular weight marker. 
Lane 2: 2 μg of purified NP-6G4 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent. Mouse IgG1 NP-6G4 Purified Hu E, F
0.1 mg / €330.00
  Acris Antibodies GmbH

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