CD2 antibody

Principal name

CD2 antibody

Alternative names for CD2 antibody

SRBC, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5

SwissProt ID

B2BB45 (Sheep), P06729 (Human), P08920 (Mouse), P08921 (Rat), P37998 (Horse), Q5MB93

Gene ID

497761 (Cd2), 914 (CD2), 12481, 100034205

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse), Bov (Bovine), Sh (Sheep), Eq (Equine), Por (Porcine), Gt (Goat), Mky (Monkey), Chimp (Chimpanzee), Macaque, Rhesus Mky (Rhesus Monkey), Bab (Baboon), Can (Canine)

Available hosts

Mouse, Rat, Rabbit

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Cytotoxicity Assay (CT), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Luminex (LMNX)

Background of CD2 antibody

Human T lymphocytes were initially distinguished from B lymphocytes by their ability to produce spontaneous rosettes with sheep red blood cells, a phenomenon mediated by the CD2 molecule, a glycosylated transmembrane receptor molecule also referred to as T11 antigen or LFA-3 antigen (leukocyte function associated antigen -3). Three functionally important epitope groups have been defined on the humans CD2 molecule, designated T111, T112, and T113, (CD2R). T111, is the epitope responsible for E-rosetting and T cell stimulation through this epitope and is mediated by an IL-2 dependent pathway (Meur et al, 1984; Knowles, 1984). Stimulation of the T112 and T113 epitopes occurs via an alternative pathway (Meur et al, 1984; Knowles, 1984).
CD2 is one of the earliest T cell lineage restricted antigens to appear during T cell differentiation and only a rare CD2+ cell can be found in the bone marrow. It is found in all T-lymphocytes and natural killer cells but not in B cells or any other cell population. CD2 binds to its counter receptor CD-58 (LFA-3), a member of the Ig gene superfamily, which located on the surface of target cells. CD2 binding to LFA-3 activates T cells and may also have a role in prothymocyte homing as it is known to mediate thymocyte-thymic epithelium adhesion. Although it is known that CD2 appears after CD7 but before CD1, its temporal relationship with CD3 is less definite, with some recent evidence suggesting that CD3 appears in the cytoplasm before CD2 (Osborn et al, 1995).
CD2 can be considered a pan-T cell antigen and is therefore useful for the identification of virtually all normal T lymphocytes. It is also very useful in the assessment of lymphoid malignancies as it is expressed in the majority of precursor and post-thymic lymphomas and leukemias and is not expressed by B neoplasms (Foon & Todd, 1986). As with other pan-T cell antigens, CD2 may be aberrantly deleted in some neoplastic T cell population, especially peripheral T cell lymphomas. Rarely slg+ B cell neoplasms have been described to form spontaneous E-rosettes but these reactions are not mediated via the CD2 receptor (Knowles, 1989).

General readings

General / background references:Whiteland JL et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). PubMed PMID: 7868861
Beyers AD et al. Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen. Immunol Rev 111:59-77 (1989). PubMed PMID: 2576418
Williams AF et al. Similarities in sequences and cellular expression between rat CD2 and CD4 antigens. J Exp Med 165:368-80 (1987). PubMed PMID: 3102667
Jefferies WA et al. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med 162:117-27 (1985). PubMed PMID: 3159821
Barclay AN Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX 2 antigens. Immunology 44:727-36 (1981). PubMed PMID: 6119291

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD2 antibody

CD2 Mouse IgG1 UMAB6 Purified Hu WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD2 antibody

Flow cytometric Analysis of Jurkat cells, using anti-CD2 antibody(UM500054),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue). Mouse IgG1 UMAB63 Purified Hu F
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD2 antibody

CD2 Mouse IgG1 UMAB6 Purified Hu WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD2 antibody

Flow cytometric Analysis of Jurkat cells, using anti-CD2 antibody(UM500054),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue). Mouse IgG1 UMAB63 Purified Hu F
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD2 mouse monoclonal antibody. (UM800017; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB86 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD2 mouse monoclonal antibody. (UM800017; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB86 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD2 mouse monoclonal antibody. (TA500394) Mouse IgG1 OTI9D1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800655) Mouse IgG1 OTI1C5 Purified Can, Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800673) Mouse IgG1 OTI3E11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD2 (25-209) antibody

AP15424PU CD2 antibody staining of Human Tonsil. Rabbit Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

CD2 (25-209) antibody

AP15424PU CD2 antibody staining of Human Tonsil. Rabbit Purified Hu P, WB
1 ml / €440.00
  Acris Antibodies GmbH

CD2 (25-209) antibody

AP15424PU CD2 antibody staining of Human Tonsil. Rabbit Purified Hu P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

CD2 antibody

Staining of mouse splenocytes with Anti-Mouse CD2 FITC.  Autofluorescence is indicated by open histogram.  Total viable cells were used for analysis. Rat RM2-5 Purified Ms F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD2 antibody

Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD2 Purified (filled histogram) followed by Anti-Mouse IgG PE .  Cells in the lymphocyte gate were used for analysis. Mouse RPA-2.10 Purified Bab, Chimp, Hu, Por F, P, WB
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD2 antibody

Immunohistochemistry staining of Paraffin Tonsil tissue by CD2 antibody (dilution: 1:10 - 1:50; visualization of staining: Cytoplasmic, membranous) Mouse IgG1 MRQ-11 Purified Hu P
1 ml / €640.00
  OriGene Technologies, Inc.

CD2 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200) Mouse IgG1 OTI4E4 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD2 antibody

CD2 Elisa with 1C5 Capture (TA600309) and Anti-Tag 1B12 Detection (TA700050) Antibodies. Substrate used: Recombinant Human CD2 (LY400671) Mouse IgG1 OTI1C5 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD2 antibody

CD2 Elisa with 3E11 Capture (TA600310) and Anti-Tag 1B12 Detection (TA700050) Antibodies. Substrate used: Recombinant Human CD2 (LY400671) Mouse IgG1 OTI3E11 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD2 antibody

CD2 Elisa with Anti-Tag 1B12 Capture (TA600050) and 1C5 Detection (TA700309) Antibodies. Substrate used: Recombinant Human CD2 (LY400671) Mouse IgG1 OTI1C5 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

CD2 antibody

CD2 Elisa with Anti-Tag 1B12 Capture (TA600050) and 3E11 Detection (TA700310) Antibodies. Substrate used: Recombinant Human CD2 (LY400671) Mouse IgG1 OTI3E11 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

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