CD20 antibody

Principal name

CD20 antibody

Alternative names for CD20 antibody

MS4A1, Leu-16, Bp35, B-cell marker, B-lymphocyte antigen CD20, Membrane-spanning 4-domains subfamily A member 1, B-lymphocyte surface antigen B1

SwissProt ID

P11836 (Human), P19437 (Mouse), Q3C2E2 (Canfa)

Gene ID

931 (MS4A1), 12482 (Ms4a1)

Available reactivities

Bov (Bovine), Hu (Human), Por (Porcine), Mky (Monkey), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Functional assay (FN), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Luminex (LMNX)

Background of CD20 antibody

The antigen recognized by CD20 MAbs is a 35-37 kDa non-glycosylated phosphoprotein, that may function as a Ca2+ ion channel. The protein structure deduced from cloned DNA sequence suggests that the CD20 antigen is a transmembrane protein that passes the membrane four times, with both the C- and the N-terminus on the cytoplasmic side and only a small part exposed on the cell surface.
Expression of CD20 antigen is specific for B lymphocytes and is present during most differentiation stages, beginning at the early pre-B cell stage and disappearing upon differentiation into plasma cells. The CD20 molecule appears to be involved in the activation and cell cycle progression of B lymphocytes. Stimulating as well as inhibitory effects in different phases of the cell cycle have been reported for different CD20 MAbs. Pokeweed mitogen induced differentiation of B lymphocytes into Ig-producing cells is inhibited by CD20 MAbs.
The CD20 antigen is also expressed on most malignant B cells. In agreement with the expression during normal B cell differentiation, all malignancies of mature B cells are CD20 positive, but early pre-B ALL cells often lack CD20 antigen. The restriction of its expression to B lineage cells, together with the lack of expression on progenitor cells, makes CD20 antigen an interesting target for (antibody-based) immunotherapy for B cell lymphoid malignancies. Moreover, CD20 antigen is expressed at relatively high density on most normal and malignant B cells and it is not susceptible to antibody induced modulation.

General readings

1. Hultin LE, Hausner MA, Hultin PM, Giorgi JV: CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes. Cytometry. 1993;14 (2):196-204.
2. Petrie RJ, Deans JP: Colocalization of the B cell receptor and CD20 followed by activation-dependent dissociation in distinct lipid rafts. J Immunol. 2002 Sep 15;169(6):2886-91.
3. Li H, Ayer LM, Polyak MJ, Mutch CM, Petrie RJ, Gauthier L, Shariat N, Hendzel MJ, Shaw AR, Patel KD, Deans JP. The CD20 calcium channel is localized to microvilli and constitutively associated with membrane rafts: antibody binding increases the affinity of the association through an epitope-dependent cross-linking-independent mechanism. J Biol Chem. 2004 May 7;279(19):19893-901.
4. Cragg MS, Walshe CA, Ivanov AO, Glennie MJ: The biology of CD20 and its potential as a target for mAb therapy. Curr Dir Autoimmun. 2005;8:140-74.
5. Glennie MJ, French RR, Cragg MS, Taylor RP: Mechanisms of killing by anti-CD20 monoclonal antibodies. Mol Immunol. 2007 Sep;44(16):3823-37.
6. Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).
7. Polyak MJ, Deans JP: Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal
antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood. 2002 May 1;99(9):3256-62.
8. Chan HT, Hughes D, French RR, Tutt AL, Walshe CA, Teeling JL, Glennie MJ, Cragg MS: CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts. Cancer Res. 2003 Sep 1;63(17):5480-9.
9. Teeling JL, Mackus WJ, Wiegman LJ, van den Brakel JH, Beers SA, French RR, van Meerten T, Ebeling S, Vink T, Slootstra JW, Parren PW, Glennie MJ, van de Winkel JG: The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20. J Immunol. 2006 Jul 1;177(1):362-71.
10. Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33.
11. Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr;15(4):e61-71.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD20 antibody

Immunohistochemical staining of paraffin-embedded lymph node tissue using anti-CD20 mouse monoclonal antibody. (Clone UMAB37, Dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB37 Purified Hu P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD20 antibody

CD20 Mouse IgG1 UMAB38 Purified Hu P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD20 antibody

CD20 Mouse IgG1 UMAB39 Purified Hu P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD20 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB58 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD20 antibody

Immunohistochemical staining of paraffin-embedded lymph node tissue using anti-CD20 mouse monoclonal antibody. (Clone UMAB37, Dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB37 Purified Hu P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD20 antibody

CD20 Mouse IgG1 UMAB38 Purified Hu P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD20 antibody

CD20 Mouse IgG1 UMAB39 Purified Hu P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD20 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB58 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD20 antibody

Immunoperoxidase of purified MaxPab antibody to MS4A1 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), F, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD20 (C-term) antibody

Formalin-Fixed and Paraffin Embedded Human tonsil stained with anti-CD20 antibody (DB 041). Kindly performed and provided by Katarina Poliakova, MD and Lubomir Straka, MD, Ph. D. from Clinical Pathology Presov, Ltd., Presov, Slovakia. Rabbit IgG E17-P Aff - Purified Hu P
0.1 ml / €280.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD20 (C-term) antibody

Formalin-Fixed and Paraffin Embedded Human tonsil stained with anti-CD20 antibody (DB 041). Kindly performed and provided by Katarina Poliakova, MD and Lubomir Straka, MD, Ph. D. from Clinical Pathology Presov, Ltd., Presov, Slovakia. Rabbit IgG E17-P Aff - Purified Hu P
0.2 ml / €370.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD20 (C-term) antibody

Formalin-Fixed and Paraffin Embedded Human tonsil stained with anti-CD20 antibody (DB 041). Kindly performed and provided by Katarina Poliakova, MD and Lubomir Straka, MD, Ph. D. from Clinical Pathology Presov, Ltd., Presov, Slovakia. Rabbit IgG E17-P Aff - Purified Hu P
0.5 ml / €460.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD20 (C-term) antibody

Formalin-Fixed and Paraffin Embedded Human tonsil stained with anti-CD20 antibody (DB 041). Kindly performed and provided by Katarina Poliakova, MD and Lubomir Straka, MD, Ph. D. from Clinical Pathology Presov, Ltd., Presov, Slovakia. Rabbit IgG E17-P Aff - Purified Hu P
1 ml / €720.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD20 (C-term) antibody

Human B cell lymphoma stained with Rabbit anti-CD20 antibody Cat.-No AM11100PU (Clone SP32). Rabbit SP32 Purified Hu F, P, WB
1 ml / €780.00
  Acris Antibodies GmbH

CD20 (C-term) antibody

Human B cell lymphoma stained with Rabbit anti-CD20 antibody Cat.-No AM11100PU (Clone SP32). Rabbit SP32 Purified Hu F, P, WB

Regular Price: 0.2 ml / €250.00

Special Price: 0.2 ml / €175.00

  Acris Antibodies GmbH

CD20 (C-term) antibody

Western blot analysis of Raji cell lysate with CD20 antibody Rabbit Purified Hu IP, P, WB
0.5 ml / €300.00
  Acris Antibodies GmbH

CD20 (C-term) antibody

Western blot analysis of Raji cell lysate with CD20 antibody Rabbit Purified Hu IP, P, WB
1 ml / €410.00
  Acris Antibodies GmbH

CD20 (C-term) antibody

Western blot analysis of Raji cell lysate with CD20 antibody Rabbit Purified Hu IP, P, WB
0.1 ml / €170.00
  Acris Antibodies GmbH

CD20 (C-term) antibody

TA305713 (0.05µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD20 antibody

Staining of normal human peripheral blood cells with Anti-Human CD20 FITC (left) and PE (right).  Appropriate isotype controls were used (open histogram).  Cells in the lymphocyte population were used for analysis. Mouse 2H7 Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

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