CD209 / DC-SIGN antibody

Principal name

CD209 / DC-SIGN antibody

Alternative names for CD209 / DC-SIGN antibody

DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, C-type lectin domain family 4 member L

SwissProt ID

Q9NNX6 (Human)

Gene ID

30835 (CD209)

Available reactivities

Hu (Human), Ms (Mouse), Sh (Sheep), Can (Canine)

Available hosts

Rabbit, Mouse, Rat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Western blot / Immunoblot (WB), Functional assay (FN), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of CD209 / DC-SIGN antibody

DC are specialized antigen presenting cells and bridge the innate and the adaptive immune system. They provide high levels of costimulation necessary for activation of both naïve and antigen-experienced T-cells. Immature DC are capable to migrate to inflammatory sites, capture antigen and process it internally to form MHC-peptide complexes. Following antigen uptake, DC undergo maturation and migrate to lymphoid organs where they can present MHC-peptide complexes to resting T-cells and drive T-cell proliferation. During differentiation and maturation of DC, several phenotypic surface markers are expressed: CD1a, CD4, CD11, CD40, CD86, and HLA-DR. Immature DC predominantly express CCR5 which enables DC to migrate to inflammatory sites, whereas mature DC express high levels of CXCR4, a receptor that facilitates migration to lymphoid organs.
DC also express DC-specific, ICAM-3 grabbing, nonintegrin (DC-SIGN), a 44 kDa C-type lectin that binds to the HIV-1 envelope glycoprotein gp120, ICAM-3 on T-cells and ICAM-2 on endothelial cells. HIV virions are able to infect cells expressing CD4 and the chemokine co-receptors CCR5 or CXCR4 and can attach to DC-SIGN to extend virion lifespan. Therefore, DC are candidates for HIV-1 infection. DC-SIGN-ICAM-3 binding is integrin-independent but calcium-dependent and antibodies reactive against DC-SIGN can be used to study DC-SIGN-ICAM3 binding.

General readings

1. Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 2000; 100:587-97.
2. Pohlmann S, Baribaud F, Lee B, et al. DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus. J. Virol. 2001;75:4664-72.
3. Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell 2000; 100:575-85.
4. Soilleux EJ, Barten R, Trowsdale J. DC-SIGN; a related gene, DC-SIGNR; and CD23 form a cluster on 19p13. J. Immunol. 2000; 165:2937-42.
5. Pohlmann S, Soilleux EJ, Baribaud F, et al. DC-SIGNR, a DCSIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans. Proc Natl Acad Sci USA. 2001; 98:2670-2675.
6. Bashirova AA, Geijtenbeek TB, van Duijnhoven GC, at el. A dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein is highly expressed on human liver sinusoidal endothelial cells and promotes HIV-1 infection. J. Exp. Med. 2001; 193:671-8.
7. Mitchell DA, Fadden AJ, Drickamer K. A novel mechanism of
carbohydrate recognition by the C-type lectins DC-SIGN and DCSIGNR: Subunit organisation and binding to multivalent ligands. J Biol Chem. 2001; 276:28939-45.
8. Mummidi S, Catano G, Lam L, et al. Extensive repertoire of membrane-bound and soluble DC-SIGN1 and DC-SIGN2 isoforms: Inter-individual variation in expression of DC-SIGN transcripts. J Biol Chem. 2001; 276:33196-212.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD209 / DC-SIGN antibody

Western blot detection of DC-SIGN in human small intestine at (A) 1 and (B) 2 ug /ml. Rabbit IgG Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD209 / DC-SIGN antibody

Western blot analysis of DC-SIGN expression in human placenta tissue lysate in the absence (lane 1) and presence (lane 2) of blocking peptide with DC-SIGN antibody at 2 ug /ml. Rabbit IgG Purified Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD209 / DC-SIGN antibody

CD209 / DC-SIGN Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD209 / DC-SIGN antibody

Western blot detection of DC-SIGN fusion protein in human placenta at (A) 1 and (B) 2 ug/mL. Mouse IgG 5D7 Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD209 / DC-SIGN antibody

Western blot detection of DC-SIGN fusion protein in human uterus tissue lysate at (A) 1 and (B) 2 ug/mL. Mouse IgG 8B6 Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD209 / DC-SIGN antibody

Staining of human monocyte-derived immature dendritic cells with 0.5 ug of Rat IgG2a K Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD209 (DC-SIGN) Purified (filled histogram) followed by Anti-Rat IgG FITC .  Cells in the large scatter population were used for analysis. Rat eB-h209 Purified Hu F
25 µg / €150.00
  OriGene Technologies, Inc.

CD209 / DC-SIGN antibody

Predicted band size: 46 kDa . Positive control: Jurkat, HUVEC, HepG2 and Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3,4: Jurkat, HUVEC, HepG2 and Hela cells Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD209 / DC-SIGN antibody

WB Suggested Anti-CD209 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD209 / DC-SIGN antibody

Host: Rabbit; Target Name: CD209; Sample Tissue: large intestine Tumor lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD209 / DC-SIGN antibody

CD209 / DC-SIGN Mouse IgG1 DCN47.5 FITC Hu F, FN
0.1 mg / €500.00
  Acris Antibodies GmbH

CD209 / DC-SIGN antibody

CD209 / DC-SIGN Mouse IgG1 DCN47.5 Purified Hu F, FN
0.1 mg / €460.00
  Acris Antibodies GmbH

CD209 / DC-SIGN antibody

CD209 / DC-SIGN Mouse IgG1 UW60.1 APC Hu F
100 Tests / €290.00
  Acris Antibodies GmbH

CD209 / DC-SIGN antibody

CD209 / DC-SIGN Mouse IgG1 UW60.1 Aff - Purified Hu F, ICC/IF
0.1 mg / €210.00
  Acris Antibodies GmbH

CD209 / DC-SIGN antibody

AP07694PU-N DC-SIGN antibody staining Formalin-Fixed Paraffin-Embedded Human Tonsil at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD209 / DC-SIGN antibody

Staining of CD209 transfected K562 cells with MOUSE ANTI HUMAN CD209: FITC (SM2219F) Mouse IgG1 MR-1 FITC Hu F
0.1 mg / €470.00
  Acris Antibodies GmbH

CD209 / DC-SIGN antibody

Flow cytometric analysis using anti CD209 antibody SM2219LE Mouse IgG1 MR-1 Low Endotoxin Hu F, FN, ICC/IF
0.5 mg / €560.00
  Acris Antibodies GmbH

CD209 / DC-SIGN antibody

Staining of CD209 transfected K562 cells with MOUSE ANTI HUMAN CD209 (SM2219P) Mouse IgG1 MR-1 Purified Hu F, ICC/IF
0.2 mg / €530.00
  Acris Antibodies GmbH

CD209 / DC-SIGN antibody

CD209 / DC-SIGN Mouse IgG1 MR-1 PE Hu F
100 Tests / €480.00
  Acris Antibodies GmbH

CD209 / DC-SIGN antibody

CD209 / DC-SIGN Mouse IgG1 MR-1 PE Hu F
25 Tests / €210.00
  Acris Antibodies GmbH

CD209 / DC-SIGN (277-293) antibody

Immunohistochemical staining of human small inestine with Rabbit anti Human CD209 antibody Cat.-No SP1337P. Rabbit Aff - Purified Hu C, ICC/IF, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

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