CD212 / IL12RB1 antibody

Principal name

CD212 / IL12RB1 antibody

Alternative names for CD212 / IL12RB1 antibody

Interleukin-12 receptor beta-1 chain, IL-12R-beta-1, IL-12 receptor beta, IL12 receptor beta component, IL-12RB1

SwissProt ID

P42701 (Human), Q60837 (Mouse)

Gene ID

3594 (IL12RB1), 16161 (Il12rb1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of CD212 / IL12RB1 antibody

A functional interleukin 12 receptor complex is composed of two ß-type cytokine receptor subunits, IL-12R ß1 (100 kDa) and IL-12R ß2 (130 kDa), each independently exhibiting a low affinity for IL-12. Co-expression leads to the formation of high-affinity IL-12-binding sites and reconstitution of IL-12-dependent signaling. IL-12R ß1 is a member of the hemopoietin receptor superfamily, bearing strong homology to gp130. IL-12 receptors are expressed primarily on activated T cells and natural killer (NK) cells, and activation of human PBMCs results in the up-regulation of IL-12R ß1 expression and IL-12 binding. Alternately, individuals that are genetically deficient in IL-12R ß1 produce greatly reduced IFN-? levels compared to healthy individuals. IL-12 induces tyrosine phosphorylation of a recently identified 85 kDa component of the IL-12 receptor complex that associates with the IL-12R ß1 subunit.

General readings

Li X., et al. (2013) . Allergy Clin. Immunol. 132 (2), 313-320Giatrakos S, et al. (2013) Cytokine 62 (2), 297-301

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD212 / IL12RB1 antibody

Sample(30 ug whole cell lysate). A:A431. 7.5% SDS PAGE. TA308386 diluted at 1:1000 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD212 / IL12RB1 antibody

(0.3ug/ml) staining of Peripharal Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD212 / IL12RB1 antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid and colon cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using IL12RB1 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD212 / IL12RB1 antibody

Predicted cell location: Cytoplasm. Positive control: Human esophagus and lung cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using IL12RB1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD212 / IL12RB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL12RB1 (RC216444, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL12RB1.(1:500) Mouse IgG1 OTI1A6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD212 / IL12RB1 antibody

Flow cytometric analysis of CD212 expression on parental cells (right) and transfectant cells (left). Open histgram indicates the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26476AF-N to the cells. Mouse IgG1 TOS Azide Free Hu F
0.1 mg / €370.00
  Acris Antibodies GmbH

CD212 / IL12RB1 antibody

Flow cytometric analysis of CD212 / IL-12R β1 expression on 293T cells (left) and Transfectant cells (right). Open histogram indicates the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26476RP-N to the cells. Mouse IgG1 TOS PE Hu F
50 Tests / €350.00
  Acris Antibodies GmbH

CD212 / IL12RB1 antibody

Western Blot analysis of IL12RB1 expression in transfected 293T cell line (H00003594-T01) by IL12RB1 MaxPab polyclonal antibody.

Lane 1: IL12RB1 transfected lysate(42.02 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

CD212 / IL12RB1 antibody

CD212 / IL12RB1 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD212 / IL12RB1 antibody

Western Blot analysis of IL12RB1 expression in transfected 293T cell line (H00003594-T02) by IL12RB1 MaxPab polyclonal antibody.

Lane 1: IL12RB1 transfected lysate(41.91 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

CD212 / IL12RB1 antibody

Immunoprecipitation of IL12RB1 transfected lysate using anti-IL12RB1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IL12RB1 MaxPab mouse polyclonal antibody (B02) (H00003594-B02). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

CD212 / IL12RB1 antibody

Western Blot analysis of IL12RB1 expression in transfected 293T cell line (H00003594-T03) by IL12RB1 MaxPab polyclonal antibody.

Lane 1: IL12RB1 transfected lysate(42.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CRISPR

Catalog No. Species Donor Vector  

Il12rb1 - mouse gene knockout kit via CRISPR

  Il12rb1 - mouse gene knockout kit via CRISPR  
Il12rb1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Il12rb1 - mouse gene knockout kit via CRISPR

  Il12rb1 - mouse gene knockout kit via CRISPR  
Il12rb1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Il12rb1 - mouse gene knockout kit via CRISPR

  Il12rb1 - mouse gene knockout kit via CRISPR  
Il12rb1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Il12rb1 - mouse gene knockout kit via CRISPR

  Il12rb1 - mouse gene knockout kit via CRISPR  
Il12rb1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD212 / IL12RB1 (transcript variant 1)

CD212 / IL12RB1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

CD212 / IL12RB1 (1-545, His-tag)

CD212 / IL12RB1 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

CD212 / IL12RB1 (1-545, His-tag)

CD212 / IL12RB1 Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

CD212 / IL12RB1 (24-545, His-tag)

CD212 / IL12RB1 Human Purified > 90 % by SDS - PAGE.
0.25 mg / €940.00
  Acris Antibodies GmbH

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