CD22 antibody

Principal name

CD22 antibody

Alternative names for CD22 antibody

SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM, Sialic acid-binding Ig-like lectin 2, B-lymphocyte cell adhesion molecule

SwissProt ID

P20273 (Human), P35329 (Mouse)

Gene ID

933 (CD22), 12483 (Cd22)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate), Bov (Bovine), Sh (Sheep), Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Rat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Frozen Sections (C), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD22 antibody

CD22 is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface simultaneously with surface IgD, and is found on most mature B cells. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via surface Ig increases CD22 expression [1]. It is more strongly expressed on prolymphocytic leukemia and HCL than in chronic lymphocytic leukemia.
B cell lineage ALL express membrane and cytoplasmic CD22.
CD22 forms a loose complex with the BcR cell antigen receptor (BcR) [2]. The cytoplasmic domain is tyrosine phosphorylated upon ligation of the BCR and associates via SH2 domains with the tyrosine phosphatase SHP-1, the tyrosine kinase Syk and phospholipase C-g1 [3,4]. CD22 down-modulates the B cell activation threshold, presumably through its association with SHP-1 and other signaling molecules [2,3]. Mice deficient in CD22 show exaggerated antibody responses to antigen and have raised levels of autoantibodies [1,5]. CD22 can also mediate cell adhesion through its interaction with cell surface molecules bearing the appropriate sialoglycoconjugates, but only when these conjugates are not on the CD22 bearing cell itself [2,6].

General readings

Cesano A. and Gayko U. Semin. Oncol. Apr. 2003 (2): 253-257.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD22 antibody

  Rabbit Monoclonal anti-CD22 Antibody  
Human Tonsil stained with anti-CD22 antibody. Rabbit IgG SP104 Purified Hu F, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  CD22 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12)  
Immunofluorescent staining of HeLa cells using anti-CD22 mouse monoclonal antibody (TA506407). Mouse IgG1 OTI1F12 Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

  Rabbit polyclonal BL-CAM (Ab-807) antibody  
Western blot analysis of extracts from 293 cells, treated with Ca2+ (40nM, 40mins), using BL-CAM (Ab-807) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  Anti-CD22 Rabbit Polyclonal Antibody  
Predicted band size: 140 kDa . Positive control: Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  Biotinylated Anti-Human sCD22 Rabbit Polyclonal Antibody  
To detect hCD22 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  Anti-Human sCD22 Rabbit Polyclonal Antibody  
To detect hCD22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CD22 (TA328277) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCD22. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  Rabbit anti-CD22 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using CD22 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  Mouse Monoclonal anti-CD22 Antibody  
Human peripheral blood lymphocytes stained with purified HIB22, followed by anti-mouse IgGs FITC Mouse HIB22 Purified Hu F, P
0.1 mg / €248.00
  OriGene Technologies, Inc.

CD22 antibody

  Mouse monoclonal Anti-CD22 Clone RFB-4  
CD22 Mouse IgG1 RFB-4 Purified Hu F, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  Mouse monoclonal Anti-SIGLEC2 Clone 4KB128  
CD22 Mouse IgG1 4KB128 Purified Hu F
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  Mouse monoclonal Anti-SIGLEC2 Clone TPD44/D11  
CD22 Mouse IgG2 TPD44/D11 Purified Hu F, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  Mouse monoclonal Anti-CD22 Clone 4KB71  
CD22 Mouse IgG 4KB71 Purified Hu P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  Mouse monoclonal Anti-CD22 Clone To15  
CD22 Mouse To15 Purified Hu F
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  Rabbit Polyclonal anti-CD22 Antibody  
Human Tonsil stained with anti-CD22 antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  CD22 mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506404) Mouse IgG1 OTI1F2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

  CD22 mouse monoclonal antibody, clone OTI1B1 (formerly 1B1)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506405) Mouse IgG1 OTI1B1 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

  CD22 mouse monoclonal antibody, clone OTI4C3 (formerly 4C3)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506412) Mouse IgG1 OTI4C3 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

  Rabbit Polyclonal CD22 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22. Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

  CD22 mouse monoclonal antibody, clone OTI2B12 (formerly 2B12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.(1:2000) Mouse IgG1 OTI2B12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

  CD22 mouse monoclonal antibody, clone OTI2E6 (formerly 2E6)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807826)(1:150) Mouse IgG2b OTI2E6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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