CD22 antibody

Principal name

CD22 antibody

Alternative names for CD22 antibody

SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM, Sialic acid-binding Ig-like lectin 2, B-lymphocyte cell adhesion molecule

SwissProt ID

P20273 (Human), P35329 (Mouse)

Gene ID

933 (CD22), 12483 (Cd22), 9332 (CD163)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate), Mky (Monkey), Bov (Bovine), Sh (Sheep), Can (Canine)

Available hosts

Mouse, Rabbit, Rat

Available applications

Flow Cytometry (F), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of CD22 antibody

CD22 is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface simultaneously with surface IgD, and is found on most mature B cells. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via surface Ig increases CD22 expression [1]. It is more strongly expressed on prolymphocytic leukemia and HCL than in chronic lymphocytic leukemia.
B cell lineage ALL express membrane and cytoplasmic CD22.
CD22 forms a loose complex with the BcR cell antigen receptor (BcR) [2]. The cytoplasmic domain is tyrosine phosphorylated upon ligation of the BCR and associates via SH2 domains with the tyrosine phosphatase SHP-1, the tyrosine kinase Syk and phospholipase C-g1 [3,4]. CD22 down-modulates the B cell activation threshold, presumably through its association with SHP-1 and other signaling molecules [2,3]. Mice deficient in CD22 show exaggerated antibody responses to antigen and have raised levels of autoantibodies [1,5]. CD22 can also mediate cell adhesion through its interaction with cell surface molecules bearing the appropriate sialoglycoconjugates, but only when these conjugates are not on the CD22 bearing cell itself [2,6].

General readings

1. Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv Immunol. 2005;88:1-50.
2. Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and
innate immunity. Mol Cell Biol. 2007 Aug;27(16):5699-710.
3. Walker JA, Smith KG: CD22: an inhibitory enigma. Immunology. 2007 Dec 7
4. Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
5. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD22 antibody

Immunofluorescent staining of HeLa cells using anti-CD22 mouse monoclonal antibody (TA506407). Mouse IgG1 OTI1F12 Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 (C-term) antibody

Human Tonsil stained with anti-CD22 Antibody Cat.-No AM10061PU (Clone SP104). Rabbit IgG SP104 Purified Can, Hu F, P
0.5 ml / €510.00
  Acris Antibodies GmbH

CD22 (C-term) antibody

Human Tonsil stained with anti-CD22 Antibody Cat.-No AM10061PU (Clone SP104). Rabbit IgG SP104 Purified Can, Hu F, P
1 ml / €730.00
  Acris Antibodies GmbH

CD22 (C-term) antibody

Human Tonsil stained with anti-CD22 Antibody Cat.-No AM10061PU (Clone SP104). Rabbit IgG SP104 Purified Can, Hu F, P
0.1 ml / €290.00
  Acris Antibodies GmbH

CD22 (C-term) antibody

Human tonsil stained with anti-CD22 antibody AP21305PU Rabbit IgG Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD22 (C-term) antibody

Human tonsil stained with anti-CD22 antibody AP21305PU Rabbit IgG Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

CD22 (C-term) antibody

Human tonsil stained with anti-CD22 antibody AP21305PU Rabbit IgG Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

CD22 antibody

Western blot analysis of extracts from 293 cells, treated with Ca2+ (40nM, 40mins), using BL-CAM (Ab-807) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD22 antibody

Predicted band size: 140 kDa . Positive control: Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD22 antibody

To detect hCD22 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

To detect hCD22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CD22 (TA328277) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCD22. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

Western blot analysis of extracts of various cell lines, using CD22 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506404) Mouse IgG1 OTI1F2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506405) Mouse IgG1 OTI1B1 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506412) Mouse IgG1 OTI4C3 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22. Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.(1:2000) Mouse IgG1 OTI2B12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807826)(1:150) Mouse IgG2b OTI2E6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.(1:2000) Mouse IgG1 OTI2A4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.(1:2000) Mouse IgG1 OTI8E6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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