CD22 antibody

Principal name

CD22 antibody

Alternative names for CD22 antibody

SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM, Sialic acid-binding Ig-like lectin 2, B-lymphocyte cell adhesion molecule

SwissProt ID

P20273 (Human), P35329 (Mouse)

Gene ID

933 (CD22), 12483 (Cd22)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Prim (Primate), Bov (Bovine), Sh (Sheep), Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Rat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Frozen Sections (C), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD22 antibody

CD22 is detected in the cytoplasm early in B cell development (late pro-B cell stage), appears on the cell surface simultaneously with surface IgD, and is found on most mature B cells. Expression is lost with terminal differentiation of B cells and is absent on plasma cells. Activation of B cells via surface Ig increases CD22 expression [1]. It is more strongly expressed on prolymphocytic leukemia and HCL than in chronic lymphocytic leukemia.
B cell lineage ALL express membrane and cytoplasmic CD22.
CD22 forms a loose complex with the BcR cell antigen receptor (BcR) [2]. The cytoplasmic domain is tyrosine phosphorylated upon ligation of the BCR and associates via SH2 domains with the tyrosine phosphatase SHP-1, the tyrosine kinase Syk and phospholipase C-g1 [3,4]. CD22 down-modulates the B cell activation threshold, presumably through its association with SHP-1 and other signaling molecules [2,3]. Mice deficient in CD22 show exaggerated antibody responses to antigen and have raised levels of autoantibodies [1,5]. CD22 can also mediate cell adhesion through its interaction with cell surface molecules bearing the appropriate sialoglycoconjugates, but only when these conjugates are not on the CD22 bearing cell itself [2,6].

General readings

Cesano A. and Gayko U. Semin. Oncol. Apr. 2003 (2): 253-257.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD22 antibody

Human Tonsil stained with anti-CD22 antibody. Rabbit IgG SP104 Purified Hu F, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD22 antibody

Immunofluorescent staining of HeLa cells using anti-CD22 mouse monoclonal antibody (TA506407). Mouse IgG1 OTI1F12 Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD22 (C-term) antibody

Human Tonsil stained with anti-CD22 Antibody Cat.-No AM10061PU (Clone SP104). Rabbit IgG SP104 Purified Can, Hu F, P
0.5 ml / €520.00
  Acris Antibodies GmbH

CD22 (C-term) antibody

Human Tonsil stained with anti-CD22 Antibody Cat.-No AM10061PU (Clone SP104). Rabbit IgG SP104 Purified Can, Hu F, P
1 ml / €750.00
  Acris Antibodies GmbH

CD22 (C-term) antibody

Human Tonsil stained with anti-CD22 Antibody Cat.-No AM10061PU (Clone SP104). Rabbit IgG SP104 Purified Can, Hu F, P
0.1 ml / €300.00
  Acris Antibodies GmbH

CD22 (C-term) antibody

Human tonsil stained with anti-CD22 antibody AP21305PU Rabbit IgG Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

CD22 (C-term) antibody

Human tonsil stained with anti-CD22 antibody AP21305PU Rabbit IgG Purified Hu P
1 ml / €530.00
  Acris Antibodies GmbH

CD22 (C-term) antibody

Human tonsil stained with anti-CD22 antibody AP21305PU Rabbit IgG Purified Hu P
0.1 ml / €200.00
  Acris Antibodies GmbH

CD22 antibody

Western blot analysis of extracts from 293 cells, treated with Ca2+ (40nM, 40mins), using BL-CAM (Ab-807) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD22 antibody

Predicted band size: 140 kDa . Positive control: Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD22 antibody

To detect hCD22 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCD22. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

To detect hCD22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CD22 (TA328277) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCD22. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

Western blot analysis of extracts of various cell lines, using CD22 antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

Human peripheral blood lymphocytes stained with purified HIB22, followed by anti-mouse IgGs FITC Mouse HIB22 Purified Hu F, P
0.1 mg / €248.00
  OriGene Technologies, Inc.

CD22 antibody

CD22 Mouse IgG1 RFB-4 Purified Hu F, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

CD22 Mouse IgG1 4KB128 Purified Hu F
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

CD22 Mouse IgG2 TPD44/D11 Purified Hu F, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

CD22 Mouse IgG 4KB71 Purified Hu P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

CD22 Mouse To15 Purified Hu F
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD22 antibody

Human Tonsil stained with anti-CD22 antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

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