CD220 / INSR antibody

Principal name

CD220 / INSR antibody

Alternative names for CD220 / INSR antibody

Insulin Receptor

SwissProt ID

P06213 (Human), P15127 (Rat), P15208 (Mouse), Q9PVZ4 (Xenla)

Gene ID

3643 (INSR), 11255 (HRH3), 16337 (Insr), 3736432 (IPR000438)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Sh (Sheep), Rb (Rabbit), Ze (Zebrafish), African clawed frog, Chk (Chicken), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Neutralisation (NEUT), Functional assay (FN), Paraffin Sections (P), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of CD220 / INSR antibody

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD; regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, binds IGF1. Ref.40 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, Ref.46 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD220 / INSR antibody

Western blot analysis of extracts from HepG2 cells, using INSR (Ab-1375) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD220 / INSR antibody

Western blot analysis of IR expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD220 / INSR antibody

CD220 / INSR Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD220 / INSR (beta chain) antibody

Anti-Insulin Receptor antibody IHC of human pancreas, islet of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

CD220 / INSR antibody

ELISA: Insulin Receptor Antibody Rabbit IgG Purified Can, Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD220 / INSR pTyr1150/1151 (incl. pos. control) antibody

Phosphospecificity Whole cell extracts of Control (Lane 1) or Insulin stimulated (Lane 2) MDA-MB-213 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. 
The Immunoblot was probed with Monoclonal InsR antibody 10C3 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 10C3 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD220 / INSR pTyr1322 (incl. pos. control) antibody

Phosphospecificity: Whole cell extracts of control (co) or Insulin, IGF1, Pervanadate stimulated MDA-MB-231 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with Monoclonal InsR antibody 21G12 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: untreated 
Lane 2: Insulin-treated 
Lane 3: IGF1-treated
Lane 4: Pervanadate-treated Mouse IgG1 21G12 Purified Can, Hu, Ms E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD220 / INSR (beta chain) (incl. pos. control) antibody

Detection of endogenous InsR: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-9H4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). 
Lane 1: A431 
Lane 2: SW480 
Lane 3: SW620 
Lane 4: HT29 
Lane 5: MCF-7 
Lane 6: MDA-MB231 
Lane 7: T47D Mouse IgG1 9H4 Purified Can, Hu, Ms, Rt C, E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD220 / INSR (beta chain) (incl. pos. control) antibody

Detection of endogenous InsR: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-11B6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A Mouse IgG1 11B6 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD220 / INSR antibody

Western blot analysis of IR antibody Cat.-No AP01347PU-N in extracts from LOVO cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD220 / INSR pTyr1185 antibody

Dot blot analysis of anti-Phospho-INSR pTyr1185 Antibody (Cat.#AP12966PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

CD220 / INSR (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD220 / INSR antibody

Western blot (WB) analysis of Insulin R antibody (Cat.-No.: AP21114PU-N) in extracts from HepG2cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CD220 / INSR antibody

CD220 / INSR Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

CD220 / INSR pThr1375 antibody

Western blot analysis of extracts from HepG2 cells using INSR (Phospho-Thr1375) Antibody AP55758PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

CD220 / INSR pThr1375 antibody

Western blot analysis of extracts from HepG2 cells using INSR (Phospho-Thr1375) Antibody AP55758PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

CD220 / INSR pTyr1355 antibody

Western blot analysis of extracts from 293 cells treated with Heatshock using Phospho-IR (Tyr1355) antibody AP55891PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu WB
0.1 ml / €340.00
  Acris Antibodies GmbH

CD220 / INSR pTyr1355 antibody

Western blot analysis of extracts from 293 cells treated with Heatshock using Phospho-IR (Tyr1355) antibody AP55891PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu WB
50 µl / €270.00
  Acris Antibodies GmbH

CD220 / INSR antibody

CD220 / INSR Mouse IgG2a 83-14 Hu IP
0.1 mg / €730.00
  Abnova Taiwan Corp.

CD220 / INSR antibody

CD220 / INSR Mouse IgG1 Jul 83 Hu IP
0.1 mg / €730.00
  Abnova Taiwan Corp.

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