CD221 / IGF1R antibody

Principal name

CD221 / IGF1R antibody

Alternative names for CD221 / IGF1R antibody

IGF-I receptor, IGF1 Receptor, Insulin-like growth factor 1 receptor

SwissProt ID

P08069 (Human), P24062 (Rat), Q05688 (Bovin), Q29000 (Pig), Q60751 (Mouse)

Gene ID

3480 (IGF1R)

Available reactivities

Hu (Human), Rb (Rabbit), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Prim (Primate)

Available hosts

Mouse, Goat, Chicken, Rabbit

Available applications

Functional assay (FN), Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of CD221 / IGF1R antibody

Biological actions of insulin and IGF1 are mediated by their respective cell surface receptors, both of which are receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The Insulin Receptor and IGF1R are heterotetrameric proteins consisting of two ligand-binding alpha subunits and two beta subunits that each contain a tyrosine kinase domain. Insulin/IGF1 binding to the extracellular domain leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains. These differences allow insulin and IGF-1 to regulate different physiological functions through receptors that share a very similar structure. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains of the Insulin Receptor/IGF1R contain a three tyrosine motif. It is generally believed that autophosphorylation within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 for the Insulin Receptor or 1135 for the IGF1R), followed by phosphorylation at the first tyrosine (1158 or 1131), then the last (1163 or 1136), upon which the Insulin Receptor or IGF1R becomes fully active.

General readings

General / background references:Takahashi MH et al. Evidence for mutual interdependence of epithelium and stromal lymphoid cells in a subset of papillary carcinomas. Br J Cancer 72:813-7 (1995). PubMed PMID: 7547225
Schumacher R et al. Signaling-competent receptor chimeras allow mapping of major insulin receptor binding domain determinants. J Biol Chem 268:1087-94 (1993). PubMed PMID: 7678247
Soos MA et al. Immunological studies of type I IGF receptors and insulin receptors: characterisation of hybrid and atypical receptor subtypes. Adv Exp Med Biol 343:145-57 (1993). PubMed PMID: 7514333

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD221 / IGF1R (alpha chain) antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD221-Low Endotoxin Cat,.-No BM2226LE (Clone 1H7). Mouse IgG1 1H7 Low Endotoxin Hu F, FN, P, WB
0.5 mg / €540.00
  Acris Antibodies GmbH

CD221 / IGF1R (alpha chain) antibody

Staining of human peripheral blood granulocytes with mouse anti human CD221 (BM2226P) Mouse IgG1 1H7 Purified Hu F, P, WB
0.2 mg / €430.00
  Acris Antibodies GmbH

CD221 / IGF1R (alpha chain) antibody

Staining of human peripheral blood granulocytes with mouse anti human CD221 (BM2226P) Mouse IgG1 1H7 Purified Hu F, P, WB
25 µg / €180.00
  Acris Antibodies GmbH

CD221 / IGF1R (alpha chain) antibody

CD221 / IGF1R Mouse IgG1 1H7 PE Hu F
100 Tests / €480.00
  Acris Antibodies GmbH

CD221 / IGF1R (alpha chain) antibody

Staining of human peripheral blood ganulocytes with mouse anti human CD221:RPE Mouse IgG1 1H7 PE Hu F
25 Tests / €210.00
  Acris Antibodies GmbH

CD221 / IGF1R antibody

Immunohistochemistry-Paraffin: IGF1 Receptor Antibody (3G5C1) [NB110-87052]-human gastric adenocarcinoma(A) golon adenocarcinoma (B), endometrial carcinoma uterus(C), ovary adenocarcinoma (D) lung carcinoma (E), stomach epithelium mucosae. Mouse IgG2b 3C8B1,3G5C1 Purified Hu E, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

CD221 / IGF1R antibody

Western Blot: IGF1 Receptor Antibody - WB analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7 Rabbit Purified Hu, Ms, Prim, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD221 / IGF1R antibody

Western blot analysis using IGF-1R antibody (Lanes 1, 2 and 3) and IGF-1R (phospho-Tyr1161) antibody (Lanes 4 and 5). Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD221 / IGF1R antibody

Predicted band size: 155 kDa . Positive control: 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: treated with the peptide Lane2: 293 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD221 / IGF1R antibody

Western blot analysis of IGF1R expression in Insulin treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD221 / IGF1R antibody

Western blot analysis of IGF1R expression in A549 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD221 / IGF1R antibody

Western blot analysis of IGF1R expression in Insulin treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD221 / IGF1R antibody

ELISA: IGF1 Receptor Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD221 / IGF1R antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF1R (RC214928, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF1R.(1:2000) Mouse IgG1 OTI4C4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD221 / IGF1R antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF1R (RC214928, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF1R.(1:500) Mouse IgG1 OTI11E4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD221 / IGF1R pTyr1316, (incl. pos. control) antibody

Phosphospecificity Whole cell extracts of control (co) or Insulin, IGF1 stimulated HeLa tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab IGF1R-2B9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane1: Co; lane 2:Insulin; lane 3: IGF1 Mouse IgG1 2B9 Purified Hu E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD221 / IGF1R (C-term) (incl. pos. control) antibody

CD221 / IGF1R Mouse IgG1 7G11 Biotin Hu WB
0.1 mg / €490.00
  Acris Antibodies GmbH

CD221 / IGF1R (C-term) (incl. pos. control) antibody

Detection of endogenous IGF1R Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab IGF1R-7G11 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 Mouse IgG1 7G11 Purified Hu WB
0.1 mg / €430.00
  Acris Antibodies GmbH

CD221 / IGF1R antibody

Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma (A), colon adenocarcinoma (B), endometrial carcinoma(uterus) (C), ovary adenocarcinoma(D), lung squamous cell carcinoma (E), stomach epithelium mucosae(F), showing membrane localization using IGF1R antibody Cat.-No AM06175SU-N with DAB staining. Mouse IgG2b 3C8B1 Ascites Hu P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CD221 / IGF1R antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using IGF1R antibody Cat.-No AM06184SU-N with DAB staining. Mouse IgG2a 3G5C1 Ascites Hu P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

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