CD223 / LAG3 antibody

Principal name

CD223 / LAG3 antibody

Alternative names for CD223 / LAG3 antibody

LAG-3, FDC, Lymphocyte activation gene 3 protein

SwissProt ID

P18627 (Human), Q5BK54 (Rat), Q61790 (Mouse)

Gene ID

16768 (Lag3), 3902 (LAG3)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat)

Available hosts

Rat, Rabbit, Mouse

Available applications

Functional assay (FN), Flow Cytometry (F), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C)

Background of CD223 / LAG3 antibody

The monoclol antibody C.42 recognizes a unique carbohydrate epitope found on follicular dendritic cells (FDCs) from a variety of species. FDCs are large cells in germil centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis. Staining with this C.42 monoclol antibody is mainly restricted to the plasma membrane but in limited cases may also be cytoplasmic (as seen in mast cells).This monoclol has been shown to be a useful reagent for both flow cytometry and IHC alysis.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD223 / LAG3 antibody

Immunohistochemistry on formalin-fixed paraffin embedded human tonsil using 1 ug/mL Mouse IgM Isotype Control (left) or 1 ug/mL Anti-Human FDC Purified (right) followed by anti-Mouse IgG Biotin and DAB visualization. Nuclei are counterstained with hematoxylin. Mouse C.42 Purified Hu F, P, WB
25 µg / €120.00
  OriGene Technologies, Inc.

CD223 / LAG3 antibody

Gel: 8%SDS-PAGE,Lysate: 40 µg,,Primary antibody: LAG3 Antibody at dilution 1/148 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 3 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD223 / LAG3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3 (RC220269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAG3. Mouse IgG2b OTI5C3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD223 / LAG3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LAG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807082)(1:150) Mouse IgG2b OTI8F6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD223 / LAG3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3 (RC220269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAG3. Mouse IgG2a OTI10E7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD223 / LAG3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3 (RC220269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAG3. Mouse IgG2b OTI8G6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD223 / LAG3 (Center) antibody

Western blot analysis of LAG3 Antibody (Center) (AP17534PU-N) in K562 cell line lysates (35ug/lane). LAG3 (arrow) was detected using the purified Pab. Rabbit Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CD223 / LAG3 antibody

CD223 / LAG3 Rat IgG1 C9B7W Low Endotoxin Ms F, FN
0.5 mg / €560.00
  Acris Antibodies GmbH

CD223 / LAG3 antibody

Staining of CD223 transfected cells with RAT ANTI MOUSE CD223 (SM2270P) Rat IgG1 C9B7W Purified Ms F
0.25 mg / €390.00
  Acris Antibodies GmbH

CD223 / LAG3 antibody

Staining of CD223 transfected cells with RAT ANTI MOUSE CD223 (SM2270PS) Rat IgG1 C9B7W Purified Ms F
0.1 mg / €240.00
  Acris Antibodies GmbH

CD223 / LAG3 antibody

Staining of CD223 transfected cells with RAT ANTI MOUSE CD223 (SM2270PS) Rat IgG1 C9B7W Purified Ms F
25 µg / €170.00
  Acris Antibodies GmbH

CD223 / LAG3 antibody

Staining of CD223 transfected cells with Rat Anti Mouse CD223:RPE (SM2270R/RT) Rat IgG1 C9B7W PE Ms F
100 Tests / €290.00
  Acris Antibodies GmbH

CD223 / LAG3 antibody

Staining of CD223 transfected cells with Rat Anti Mouse CD223:RPE (SM2270R/RT) Rat IgG1 C9B7W PE Ms F
25 Tests / €210.00
  Acris Antibodies GmbH

CD223 / LAG3 antibody

CD223 / LAG3 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

CD223 / LAG3 antibody

Western Blot analysis of LAG3 expression in transfected 293T cell line (H00003902-T02) by LAG3 MaxPab polyclonal antibody.

Lane 1: LAG3 transfected lysate(39.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

CD223 / LAG3 (C-term) antibody

CD223 / LAG3 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CD223 / LAG3 antibody

Flow Cytometry: CD223 Antibody (C9B7W) [NB100-63601] - Staining of CD223 transfected cells with RAT ANTI MOUSE CD223 Rat IgG1 C9B7W Purified Ms F
0.125 mg / €340.00
  Novus Biologicals Inc.

CD223 / LAG3 antibody

Immunohistochemistry-Paraffin: Lymphocyte Activation Gene 3 Antibody [NBP1-85781] Immunohistochemical staining of human tonsil shows disinct cytoplasmic positivity in a small subset of cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

CD223 / LAG3 (C-term DDK/His tag)

CD223 / LAG3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

CD223 / LAG3 (C-term DDK/His tag)

CD223 / LAG3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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