CD227 / Mucin-1 / MUC1 antibody

Principal name

CD227 / Mucin-1 / MUC1 antibody

Alternative names for CD227 / Mucin-1 / MUC1 antibody

MUC-1, PEMT, Episialin, EMA, H23AG, PUM, DF3, CA 15-3, Polymorphic epithelial mucin, Tumor-associated mucin, Carcinoma-associated mucin, Tumor-associated epithelial membrane antigen, Peanut-reactive urinary mucin, Breast carcinoma-associated antigen DF3

SwissProt ID

P15941 (Human), Q02496 (Mouse), Q8WML4 (Bovin)

Gene ID

4582 (MUC1), 4286 (MITF)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Rb (Rabbit), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Immunoelectron Microscopy (EM), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Luminex (LMNX)

Background of CD227 / Mucin-1 / MUC1 antibody

Mucin 1 (Muc1) is a large transmembrane glycoprotein that is overexpressed by a majority of carcinomas. High expression of MUC1 is associated with aggressive tumors, and MUC1 antigen is used as a marker to monitor disease progression in breast cancer patients. Mucin is a mucopolysaccharidic matrix, its expression is widely varied during cell differentitiaon and inflammation. The extent of Glycosylation determines essential biological functions of epithelial mucins in conditions of health and disease. The mucin MUC1 is a candidate for use in specific immunotherapy against breast cancer. Mucin also protects the covering of gut epithelium where it is protected from proteolytic digestion by extensive glycosylation on the mucin molecule (1). The quantity and quality of mucins are also affected in inflammatory bowel disease (IBD) both because of a reduction in the number of goblet cells and a decrease in the number of sugar residues per oligosaccharide side chain (2). There are several mucin phenotypes examined in the gut (mucin 1-mucin 6), the expression of these mucin in various type of minute well-differentiated type adenocarcinoma has been examined for diagnostic purposes (3). The mucin knockout mice showed significant decrease in cholesterol uptake with no effect on fatty acif absorption (4).
In breast cancer, overexpression of MUC1 suggest to contributes to cancer progression and metastasis. It has been shown that naturally occurring cancer preventative, indole-3-carbinol (I3C), inhibits the expression of MUC1 in breast cancer estrogen responsive and non-responsive cells (5). Unlike normal tissue where MUC exists as heavily glycosylated form, the disease- or tumor-associated MUC molecules are underglycosylated. Such underglycosylation of the core protein in cancer tissues exposes new epitopes on the cell surface that are unique to cancer tissues (6).
Mucins are large glycoproteins characterized by mucin domains that show little sequence conservation and that are rich in the amino acids Ser, Thr and Pro. The sequentially-repeating nature of the core protein 20 a.a. polypeptide chain on the surface of malignant cells makes it a potential target for immunotherapy.

General readings

1. Uen,Y.H., Lin,S.R., Wu,C.H., Hsieh,J.S., Lu,C.Y., Yu,F.J., Huang,T.J. and Wang,J.Y.
Clinical significance of MUC1 and c-Met RT-PCR detection of circulating tumor cells in patients with gastric carcinoma
2. Lu,W., Hisatsune,A., Koga,T., Kato,K., Kuwahara,I., Lillehoj,E.P., Chen,W., Cross,A.S., Gendler,S.J., Gewirtz,A.T. and Kim,K.C.
Cutting edge: enhanced pulmonary clearance of Pseudomonas aeruginosa by Muc1 knockout mice
3. Janssen,R., Kruit,A., Grutters,J.C., Ruven,H.J., Gerritsen,W.B. and van den Bosch,J.M.
The mucin-1 568 adenosine to guanine polymorphism influences serum Krebs von den Lungen-6 levels
4. Ruvoen-Clouet,N., Mas,E., Marionneau,S., Guillon,P., Lombardo,D. and Le Pendu,J.
Bile-salt-stimulated lipase and mucins from milk of 'secretor' mothers inhibit the binding of Norwalk virus capsids to their carbohydrate ligands
5. Wei,X., Xu,H. and Kufe,D., et al.
MUC1 oncoprotein stabilizes and activates estrogen receptor alpha
6. , Lan M.S., Batra S.K., Qi W.-N., Metzgar R.S., Hollingsworth M.A.
Cloning and sequencing of a human pancreatic tumor mucin cDNA.
7. , Ligtenberg M.J.L., Vos H.L., Gennissen A.M.C., Hilkens J.
Episialin, a carcinoma-associated mucin, is generated by a polymorphic gene encoding splice variants with alternative amino termini.
8. , Gendler S.J., Lancaster C.A., Taylor-Papadimitriou J., Duhig T., Peat N., Burchell J., Pemberton L., Lalani E.-N., Wilson D.
Molecular cloning and expression of human tumor-associated polymorphic epithelial mucin.
9. , Lancaster C.A., Peat N., Duhig T., Wilson D., Taylor-Papadimitriou J., Gendler S.J.
Structure and expression of the human polymorphic epithelial mucin gene: an expressed VNTR unit.
10. , Wreschner D.H., Hareuveni M., Tsarfaty I., Smorodinsky N., Horev J., Zaretsky J., Kotkes P., Weiss M., Lathe R., Dion A., et al.
Human epithelial tumor antigen cDNA sequences. Differential splicing may generate multiple protein forms.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD227 / Mucin-1 / MUC1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MUC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800790) Mouse IgG1 OTI2F6 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD227 / Mucin-1 / MUC1 (C-term) antibody

Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Ig G22-L Purified Hu P
50 µl / €470.00
  Acris Antibodies GmbH

CD227 / Mucin-1 / MUC1 antibody

Formalin-Fixed, Paraffin-Embedded Human breast cancer stained with CD227 / Mucin-1 / MUC1 Antibody Cat.-No AM32842PU (Clone GP1.4). Note cytoplasmic and membrane staining. Mouse IgG1 GP1.4 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

CD227 / Mucin-1 / MUC1 antibody

Formalin-Fixed, Paraffin-Embedded Human breast cancer stained with CD227 / Mucin-1 / MUC1 Antibody Cat.-No AM32842PU (Clone GP1.4). Note cytoplasmic and membrane staining. Mouse IgG1 GP1.4 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

CD227 / Mucin-1 / MUC1 antibody

Formalin-Fixed, Paraffin-Embedded Human breast cancer stained with CD227 / Mucin-1 / MUC1 Antibody Cat.-No AM32842PU (Clone GP1.4). Note cytoplasmic and membrane staining. Mouse IgG1 GP1.4 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

CD227 / Mucin-1 / MUC1 antibody

Immunohistochemical staining of paraffin-embedded Human normal bladder tissue and bladder cancer tissue using anti-MUC1 mouse monoclonal antibody. (UM800008; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG2b UMAB57 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

CD227 / Mucin-1 / MUC1 antibody

Immunohistochemical staining of paraffin-embedded Human normal bladder tissue and bladder cancer tissue using anti-MUC1 mouse monoclonal antibody. (UM800008; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG2b UMAB57 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

CD227 / Mucin-1 / MUC1 antibody

Western Blot: MUC1 Antibody [TA336810] - HepG2 Whole cell lysates. Rabbit Purified Bov, Eq, GP, Hu, Ms, Por, Rt C, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD227 / Mucin-1 / MUC1 antibody

MUC1 ELISA with 1F3 Capture (TA600445) and 4A5 Detection (TA700446) Antibodies. Substrate used: Recombinant Human MUC1 (TP321390) Mouse IgG1 OTI1F3 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD227 / Mucin-1 / MUC1 antibody

MUC1 ELISA with 2A5 Capture (TA600446) and 4A5 Detection (TA700446) Antibodies. Substrate used: Recombinant Human MUC1 (TP321390) Mouse IgG1 OTI2A5 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD227 / Mucin-1 / MUC1 antibody

MUC1 ELISA with 2A5 Capture (TA600446) and 2E3 Detection (TA700445) Antibodies. Substrate used: Recombinant Human MUC1 (TP321390) Mouse IgG1 OTI2E3 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

CD227 / Mucin-1 / MUC1 antibody

MUC1 ELISA with 2A5 Capture (TA600446) and 4A5 Detection (TA700446) Antibodies. Substrate used: Recombinant Human MUC1 (TP321390) Mouse IgG1 OTI4A5 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

CD227 / Mucin-1 / MUC1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MUC1 mouse monoclonal antibody. (TA800769) Dilution: 1:150 Mouse IgG1 OTIF3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD227 / Mucin-1 / MUC1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MUC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800794) Mouse IgG2a OTI2E3 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

CD227 / Mucin-1 / MUC1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MUC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800838) Mouse IgG2b OTI4A5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD227 / Mucin-1 / MUC1 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MUC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800938) Mouse IgG2a OTI3A7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CD227 / Mucin-1 / MUC1 antibody

Human stomach; . Sample Type :. Human stomach. Primary Antibody Dilution :. 1:1000. Secondary Antibody :. Anti-rabbit-HRP. Secondary Antibody Dilution :. 1:5000. Color/Signal Descriptions :. Brown: MUC1 Blue: Nucleus. Gene Name :. MUC1. Submitted by :. Dr. Sara Linden, University of Gothenburg; MUC1 antibody - C-terminal region (ARP41445_P050) in Human stomach cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

CD227 / Mucin-1 / MUC1 antibody

Human stomach; . Sample Type :. Human stomach. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Anti-rabbit-HRP. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. Brown: MUC1 Blue: Nucleus. Gene Name :. MUC1. Submitted by :. Dr. Sara Linden, University of Gothenburg; MUC1 antibody - C-terminal region (ARP41446_T100) in Human stomach cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

CD227 / Mucin-1 / MUC1 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human stomach tissue (4 µm) stained with anti-CD227 antibody (DB 048) shows strong positive immunostaining of epithelial cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG G22-L Aff - Purified Hu P
0.1 ml / €220.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD227 / Mucin-1 / MUC1 (C-term) antibody

Formalin-Fixed, Paraffin-Embedded Human stomach tissue (4 µm) stained with anti-CD227 antibody (DB 048) shows strong positive immunostaining of epithelial cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG G22-L Aff - Purified Hu P
0.2 ml / €290.00
  DB Biotech, spol. s r.o.

Sample sizes available

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