CD230 / PrP antibody

Principal name

CD230 / PrP antibody

Alternative names for CD230 / PrP antibody

Major prion protein, PrP27-30, PrP33-35C, ASCR, PRNP, PRIP

SwissProt ID

O18754 (Felca), O46501 (Canfa), P04156 (Human), P04925 (Mouse), P10279 (Bovin), P13852 (Rat), P23907 (Sheep)

Gene ID

5621 (PRNP), 493887, 281427, 19122 (Prnp)

Available reactivities

Bov (Bovine), Deer, Elk, Hu (Human), Sh (Sheep), Ms (Mouse), Rt (Rat), Fe (Feline), Hst (Hamster), Por (Porcine), Can (Canine), Gt (Goat)

Available hosts

Mouse, Rabbit, Goat, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of CD230 / PrP antibody

Prion protein PrP is a membrane glycosylphosphatidylinositol anchored glycoprotein that tends to aggregate into rod like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt Jakob disease, fatal familial insomnia, Gerstmann Straussler disease, Huntington disease like 1, and kuru. Two transcript variants encoding the same protein have been found for this gene.

All prion diseases share the same molecular pathogenic mechanism that involves conversion of normal cellular prion protein (PrPc) into a form that is insoluble in non ionic detergent and partially resistant to proteases (PrPsc). Both PrPsc and PrPc are encoded within a single exon of a chromosomal gene as a protein of ~ 250 amino acids. Many mammalian PrPs have a 22 amino acid N terminal signal sequence and 23 amino acid C terminal signal sequence encoding for attachment of a glycosylphosphatidylinositol anchor. The mature protein of 209 amino acids contains one disulfide bond and has two sites of asparagine linked glycosylation.

General readings

1. Bounhar, Y., Zhang, Y., Goodyer, C. G., LeBlanc, A.Prion protein protects human neurons against
Bax-mediated apoptosis.J. Biol. Chem. 276: 39145-39149, 2001.
2. Brown, P., Galvez, S., Goldfarb, L. G., Nieto, A., Cartier, L., Gibbs, C. J., Jr., Gajdusek, D. C.Familial
Creutzfeldt-Jakob disease in Chile is associated with the codon 200 mutation of the PRNP amyloid
precursor gene on chromosome 20.J. Neurol. Sci. 112: 65-67, 1992.
3. Puckett, C., Concannon, P., Casey, C., Hood, L.Genomic structure of the human prion protein
gene.Am. J. Hum. Genet. 49: 320-329, 1991.

  1. Quick-Filters:
  2. Cited (2)

Items 1 to 20 of 44 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD230 / PrP antibody

Rat brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Prion Protein, clone 2G11 (1 µg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Prion Protein (~27 kDa) Mouse IgG2a 2G11 Purified Hu, Ms, Rt, Sh E, P, WB
0.25 mg / €430.00
  Acris Antibodies GmbH

CD230 / PrP antibody

Western blotting analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using anti-PrP antibody (clone EM-20). CJD positive patient has proteinase K resistent prion protein.
Lane 1, 4: Samples with proteinase K treatment
Lane 2, 3: Samples without proteinase K treatment Mouse IgG2a EM-20 Aff - Purified Hu P, WB
0.1 mg / €310.00
  Acris Antibodies GmbH

CD230 / PrP (N-term) antibody

Western blot analysis of Prion protein using Prion antibody (DB 033) was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 μg of total protein per lane) of PrP-KO CF-10 cells (A), Mouse hippocampus (B) and HEK293 cells, transfected with Mouse prion protein bearing 3F4 epitope (C), Lane D corresponds to 30 ng recombinant MoPrP. Rabbit T16-R Aff - Purified Bov, Hu, Ms, Rt C, E, IP, P, WB
0.1 ml / €510.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD230 / PrP (N-term) antibody

Western blot analysis of Prion protein using Prion antibody (DB 033) was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 μg of total protein per lane) of PrP-KO CF-10 cells (A), Mouse hippocampus (B) and HEK293 cells, transfected with Mouse prion protein bearing 3F4 epitope (C), Lane D corresponds to 30 ng recombinant MoPrP. Rabbit T16-R Aff - Purified Bov, Hu, Ms, Rt C, E, IP, P, WB
50 µl / €350.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD230 / PrP antibody

Western blot analysis of Prion protein using Prion antibody (DB 033) was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 μg of total protein per lane) of PrP-KO CF-10 cells (A), Mouse hippocampus (B) and HEK293 cells, transfected with Mouse prion protein bearing 3F4 epitope (C), Lane D corresponds to 30 ng recombinant MoPrP. Rabbit V21-V Aff - Purified Bov, Hu, Ms, Rt C, E, IP, P, WB
0.1 ml / €530.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD230 / PrP antibody

Western blot analysis of Prion protein using Prion antibody (DB 033) was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 μg of total protein per lane) of PrP-KO CF-10 cells (A), Mouse hippocampus (B) and HEK293 cells, transfected with Mouse prion protein bearing 3F4 epitope (C), Lane D corresponds to 30 ng recombinant MoPrP. Rabbit V21-V Aff - Purified Bov, Hu, Ms, Rt C, E, IP, P, WB
50 µl / €350.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD230 / PrP (C-term) antibody

Western blot analysis of Prion protein using Prion antibody (DB 081) was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 μg of total protein per lane) of PrP-KO CF-10 cells (A), Mouse hippocampus (B) and HEK293 cells, transfected with Mouse prion protein bearing 3F4 epitope (C), Lane D corresponds to 30 ng recombinant MoPrP. Rabbit C16-S Aff - Purified Bov, Hu, Ms C, E, IP, P, WB
0.1 ml / €530.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD230 / PrP (C-term) antibody

Western blot analysis of Prion protein using Prion antibody (DB 081) was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 μg of total protein per lane) of PrP-KO CF-10 cells (A), Mouse hippocampus (B) and HEK293 cells, transfected with Mouse prion protein bearing 3F4 epitope (C), Lane D corresponds to 30 ng recombinant MoPrP. Rabbit C16-S Aff - Purified Bov, Hu, Ms C, E, IP, P, WB
50 µl / €350.00
  DB Biotech, spol. s r.o.

Sample sizes available

CD230 / PrP (143-153) antibody

TA302683 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Por E, WB
20 µg / €200.00
  Acris Antibodies GmbH

CD230 / PrP (143-153) antibody

TA302683 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Por E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD230 / PrP antibody

Western blot analysis of extracts of various cell lines, using PRNP antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD230 / PrP antibody

Western Blot: Prion protein Antibody [TA336429] - analysis of BSE in (A) recombinant fusion protein containing amino acids 142-148 and (B) fusion partner without these amino acids, using this antibody. 5 ug/ml. Rabbit Purified Bov, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD230 / PrP antibody

Western Blot: Prion protein Antibody [TA336430] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 162-170 and (B) fusion partner without these amino acids, using this antibody at 5 ug/ml. Rabbit Purified Bov, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD230 / PrP antibody

Western Blot: Prion protein Antibody [TA336431] - analysis of BSE in (A) recombinant fusion protein containing amino acids 217-229 and (B) fusion partner without these amino acids, using this antibody. 5 ug/ml. Rabbit Purified Bov, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD230 / PrP antibody

Host: Rabbit; Target Name: Prnp; Sample Tissue: Mouse Kidney lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Gt, Hu, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

CD230 / PrP antibody

ELISA: Prion protein Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD230 / PrP (93-109) antibody

CD230 / PrP Mouse IgG2a 6D11 Purified Bov, Hst, Hu, Ms, Sh C, E, WB
0.1 ml / €1,030.00
  Acris Antibodies GmbH

CD230 / PrP antibody

CD230 / PrP Mouse IgG1 5121 Purified Bov, Sh E, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

CD230 / PrP antibody

CD230 / PrP Goat IgG Serum Bov, Hst, Hu, Sh E, P, WB
0.1 ml / €330.00
  Acris Antibodies GmbH

CD230 / PrP antibody

CD230 / PrP Goat IgG Serum Bov, Hst, Hu, Sh P
10 µl / €200.00
  Acris Antibodies GmbH

Items 1 to 20 of 44 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn