CD235a / GYPA antibody

Principal name

CD235a / GYPA antibody

Alternative names for CD235a / GYPA antibody

Glycophorin-A, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein

SwissProt ID

P02724 (Human)

Gene ID

2993 (GYPA)

Available reactivities

Hu (Human), Bov (Bovine), Rt (Rat)

Available hosts

Mouse, Rat, Goat, Rabbit

Available applications

Frozen Sections (C), Flow Cytometry (F), Paraffin Sections (P), Agglutination (AGG), Western blot / Immunoblot (WB), Cytotoxicity Assay (CT), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of CD235a / GYPA antibody

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB.

General readings

Tolia NH, Enemark EJ, Sim BK, Joshua-Tor L. Structural Basis for the EBA-175 Erythrocyte Invasion Pathway of the Malaria Parasite Plasmodium falciparum. Cell. 2005 Jul 29;122(2):183-93. PMID: 16051144

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD235a / GYPA antibody

CD235a / GYPA Rabbit IgG Purified Hu E, IP, WB
1 ml / €370.00
  Acris Antibodies GmbH

CD235a / GYPA (N-term) antibody

CD235a / GYPA Mouse IgG2b HIR2 Purified Hu AGG, C, F, P
0.1 mg / €220.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM SPM183 Supernatant Bov, Hu AGG, C, ICC/IF, P
1.0 ml / €360.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM SPM183 Supernatant Bov, Hu AGG, C, ICC/IF, P
0.5 ml / €290.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM SPM183 Supernatant Bov, Hu AGG, C, ICC/IF, P
0.1 ml / €150.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM A63-C/A9 Supernatant Bov, Hu C, ICC/IF, P
1 ml / €360.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM A63-C/A9 Supernatant Bov, Hu C, ICC/IF, P
0.5 ml / €290.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM A63-C/A9 Supernatant Bov, Hu C, ICC/IF, P
0.1 ml / €150.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

TA303062 (1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu P, WB
20 µg / €200.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

TA303062 (1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD235a / GYPA antibody

CD235a / GYPA Mouse HIR2 (also GA-R2) Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD235a / GYPA antibody

Predicted band size: 16 kDa . Positive control: Human liver tissue lysate. Recommended dilution: 1/ 200-1000. (Gel: 12%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD235a / GYPA antibody

CD235a / GYPA Mouse IgG2a A84-B/H2 Purified Hu C, E
0.1 mg / €300.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM A63-B/C2 Purified Hu AGG, C
0.1 mg / €300.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Representative Data
NAM10-6G4 (anti-CD235a) was analyzed by flow cytometry using a diluted blood sample (100x) from a healthy human volunteer. Direct staining was performed using 10 µl of the FITC-conjugated monoclonal antibody preparation and 100 µl of the diluted blood sample.
N.B. No lysing solution was used. Mouse IgG2a NaM10-6G4 FITC Hu F
100 Tests / €350.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Representative Data
NAM10-6G4 (anti-CD235a) was analyzed by flow cytometry using a diluted blood sample (100x) from a healthy human volunteer. Direct staining was performed using 10 µl of the FITC-conjugated monoclonal antibody preparation and 100 µl of the diluted blood sample.
N.B. No lysing solution was used. Mouse IgG2a NaM10-6G4 Aff - Purified Hu C, F
1 ml / €240.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM GPHN02 Supernatant Bov, Hu C, ICC/IF, P
1 ml / €360.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM GPHN02 Supernatant Bov, Hu C, ICC/IF, P
0.5 ml / €290.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM GPHN02 Supernatant Bov, Hu C, ICC/IF, P
0.1 ml / €150.00
  Acris Antibodies GmbH

CD235a / GYPA (139-150) antibody

Human Red Blood Cells (formalin-fixed, paraffin-embedded) stained with GYPA antibody AP22500PU-N at 2 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Hu, Prim E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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