CD235a / GYPA antibody

Principal name

CD235a / GYPA antibody

Alternative names for CD235a / GYPA antibody

Glycophorin-A, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein

SwissProt ID

P02724 (Human)

Gene ID

2993 (GYPA)

Available reactivities

Hu (Human), Prim (Primate), Bov (Bovine), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Flow Cytometry (F), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Agglutination (AGG), Immunocytochemistry/Immunofluorescence (ICC/IF), Cytotoxicity Assay (CT)

Background of CD235a / GYPA antibody

Kit Component:
- KN202751G1, GYPA gRNA vector 1 in pCas-Guide vector
- KN202751G2, GYPA gRNA vector 2 in pCas-Guide vector
- KN202751D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Maijenburg MW, Kleijer M, Vermeul K, Mul EP, van Alphen FP, van der Schoot CE, et al. The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging. Haematologica. 2012 Feb;97(2):179-83. doi: 10.3324/haematol.2011.047753. Epub 2011 Oct 11. PubMed PMID: 21993672.
2. Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, et al. Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients. PLoS One. 2013 Nov 25;8(11):e81002. doi: 10.1371/journal.pone.0081002. eCollection 2013. PubMed PMID: 24282562.
3. Yamauchi T, Takenaka K, Urata S, Shima T, Kikushige Y, Tokuyama T, et al. Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment. Blood. 2013 Feb 21;121(8):1316-25. doi: 10.1182/blood-2012-06-440354. Epub 2013 Jan 4. PubMed PMID: 23293079.
4. Alijotas-Reig J, Palacio-Garcia C, Llurba E, Vilardell-Tarres M. Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review. Fertil Steril. 2013 Feb;99(2):441-9. doi: 10.1016/j.fertnstert.2012.10.009. Epub 2012 Nov 2. PubMed PMID: 23122952.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CD235a / GYPA antibody

CD235a / GYPA Rabbit IgG Purified Hu E, IP, WB
1 ml / €370.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

  Goat Polyclonal Antibody against GYPA  
TA303062 (1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD235a / GYPA antibody

  Mouse Anti-Human CD235a (Glycophorin A) Purified (100 ug)  
CD235a / GYPA Mouse HIR2 (also GA-R2) Purified Hu F
0.1 mg / €120.00
  OriGene Technologies, Inc.

CD235a / GYPA antibody

  Anti-GYPA Rabbit Polyclonal Antibody  
Predicted band size: 16 kDa . Positive control: Human liver tissue lysate. Recommended dilution: 1/ 200-1000. (Gel: 12%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CD235a / GYPA antibody

  Mouse monoclonal Anti-GlycophorinA Clone JC159  
CD235a / GYPA Mouse IgG1 JC159 Purified Hu F, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

CD235a / GYPA antibody

CD235a / GYPA Mouse IgG2a A84-B/H2 Purified Hu C, E
0.1 mg / €310.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgM A63-B/C2 Purified Hu AGG, C
0.1 mg / €310.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Representative Data
NAM10-6G4 (anti-CD235a) was analyzed by flow cytometry using a diluted blood sample (100x) from a healthy human volunteer. Direct staining was performed using 10 µl of the FITC-conjugated monoclonal antibody preparation and 100 µl of the diluted blood sample.
N.B. No lysing solution was used. Mouse IgG2a NaM10-6G4 FITC Hu F
100 Tests / €350.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Representative Data
NAM10-6G4 (anti-CD235a) was analyzed by flow cytometry using a diluted blood sample (100x) from a healthy human volunteer. Direct staining was performed using 10 µl of the FITC-conjugated monoclonal antibody preparation and 100 µl of the diluted blood sample.
N.B. No lysing solution was used. Mouse IgG2a NaM10-6G4 Aff - Purified Hu C, F
1 ml / €240.00
  Acris Antibodies GmbH

CD235a / GYPA (139-150) antibody

Human Red Blood Cells (formalin-fixed, paraffin-embedded) stained with GYPA antibody AP22500PU-N at 2 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD235a / GYPA (N-term) antibody

Human Placenta, Vessel with Erythrocytes (formalin-fixed, paraffin-embedded) stained with GYPA antibody AP22594PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu E, F, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Rabbit IgG FITC Hu F
0.1 mg / €370.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Western blot analysis using AP31671PU-N (Purified Anti-Glycophorin A pAb) at a dilution of 1/100 on human RBCs at various concentrations (GPA = Glycophorin A, GPB = Glycophorin B). Rabbit IgG Purified Hu E, F, WB
0.25 mg / €370.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

CD235a / GYPA Mouse IgG1 BRIC-256 Purified Hu C, F, WB
0.1 mg / €410.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Formalin fixed paraffin embedded human tonsils stained with glycophorin A monoclonal antibody (Cat.#DM066). Mouse IgG1 JC159 Supernatant Hu P
1 ml / €560.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Formalin fixed paraffin embedded human tonsils stained with glycophorin A monoclonal antibody (Cat.#DM066). Mouse IgG1 JC159 Supernatant Hu P
0.5 ml / €370.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Surface staining of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) FITC. Mouse IgG1 JC159 FITC Hu, Rt F
100 Tests / €260.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Surface staining of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) PE. Mouse IgG1 JC159 Purified Hu, Rt C, F, P
0.1 mg / €210.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Surface staining of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) PE. Mouse IgG1 JC159 PE Hu, Rt F
100 Tests / €290.00
  Acris Antibodies GmbH

CD235a / GYPA antibody

Staining of human peripheral blood erythrocytes with Rat Anti Human CD235a-FITC (SM1473F/FT). Rat IgG2b YTH89.1 FITC Hu F
0.1 mg / €380.00
  Acris Antibodies GmbH

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